Salivary proteomic profile of young healthy subjects
Background: The incidence of noncommunicable diseases (NCDs) has been rapidly ramped up worldwide. Hence, there is an urgent need to non-invasively detect NCDs possibly by exploiting saliva as a ‘liquid biopsy’ to identify biomarkers of the health status. Since, the absence of standardized procedure...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1327233/full |
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| author | P. Dongiovanni M. Meroni Gilda Aiello A. D’Amato N. Cenzato S. Casati G. Damiani C. Fenoglio D. Galimberti D. Galimberti E. Grossi D. Prati G. Lamorte C. Bianco L. Valenti A. Soggiu S. Zapperi C. A. M. La Porta M. Del Fabbro M. Del Fabbro G. M. Tartaglia G. M. Tartaglia |
| author_facet | P. Dongiovanni M. Meroni Gilda Aiello A. D’Amato N. Cenzato S. Casati G. Damiani C. Fenoglio D. Galimberti D. Galimberti E. Grossi D. Prati G. Lamorte C. Bianco L. Valenti A. Soggiu S. Zapperi C. A. M. La Porta M. Del Fabbro M. Del Fabbro G. M. Tartaglia G. M. Tartaglia |
| author_sort | P. Dongiovanni |
| collection | DOAJ |
| description | Background: The incidence of noncommunicable diseases (NCDs) has been rapidly ramped up worldwide. Hence, there is an urgent need to non-invasively detect NCDs possibly by exploiting saliva as a ‘liquid biopsy’ to identify biomarkers of the health status. Since, the absence of standardized procedures of collection/analysis and the lack of normal ranges makes the use of saliva still tricky, our purpose was to outline a salivary proteomic profile which features healthy individuals.Methods: We collected saliva samples from 19 young blood donors as reference population and the proteomic profile was investigated through mass-spectrometry.Results: We identified 1,004 proteins of whose 243 proteins were shared by all subjects. By applying a data clustering approach, we found a set of six most representative proteins across all subjects including Coronin-1A, F-actin-capping protein subunit alpha, Immunoglobulin J chain, Prosaposin, 78 kDa glucose-regulated protein and Heat shock 70 kDa protein 1A and 1B.Conclusion: All of these proteins are involved in immune system activation, cellular stress responses, proliferation, and invasion thus suggesting their use as biomarkers in patients with NCDs. |
| first_indexed | 2024-03-09T10:49:29Z |
| format | Article |
| id | doaj.art-f0a4bd289d2847cd970a9c52f7ffa6c2 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-03-09T10:49:29Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-f0a4bd289d2847cd970a9c52f7ffa6c22023-12-01T09:36:19ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2023-11-011010.3389/fmolb.2023.13272331327233Salivary proteomic profile of young healthy subjectsP. Dongiovanni0M. Meroni1Gilda Aiello2A. D’Amato3N. Cenzato4S. Casati5G. Damiani6C. Fenoglio7D. Galimberti8D. Galimberti9E. Grossi10D. Prati11G. Lamorte12C. Bianco13L. Valenti14A. Soggiu15S. Zapperi16C. A. M. La Porta17M. Del Fabbro18M. Del Fabbro19G. M. Tartaglia20G. M. Tartaglia21Medicine and Metabolic Diseases, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Milan, ItalyMedicine and Metabolic Diseases, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Milan, ItalyDepartment of Human Science and Quality of Life Promotion, Telematic University San Raffaele, Rome, ItalyDepartment of Pharmaceutical Sciences, University of Milan, Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, ItalyNeurodegenerative Diseases Unit, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Milan, ItalyVilla Santa Maria Foundation, Tavernerio, ItalyBiological Resource Center, Department of Transfusion Medicine, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Milan, ItalyBiological Resource Center, Department of Transfusion Medicine, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Milan, ItalyBiological Resource Center, Department of Transfusion Medicine, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Milan, ItalyBiological Resource Center, Department of Transfusion Medicine, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, ItalyDepartment of Physics, Center for Complexity and Biosystems, University of Milan, Milan, ItalyDepartment of Environmental Science and Policy, Center for Complexity and Biosystems, University of Milan, Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy0SC Maxillo-Facial Surgery and Dentistry, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy0SC Maxillo-Facial Surgery and Dentistry, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Milan, ItalyBackground: The incidence of noncommunicable diseases (NCDs) has been rapidly ramped up worldwide. Hence, there is an urgent need to non-invasively detect NCDs possibly by exploiting saliva as a ‘liquid biopsy’ to identify biomarkers of the health status. Since, the absence of standardized procedures of collection/analysis and the lack of normal ranges makes the use of saliva still tricky, our purpose was to outline a salivary proteomic profile which features healthy individuals.Methods: We collected saliva samples from 19 young blood donors as reference population and the proteomic profile was investigated through mass-spectrometry.Results: We identified 1,004 proteins of whose 243 proteins were shared by all subjects. By applying a data clustering approach, we found a set of six most representative proteins across all subjects including Coronin-1A, F-actin-capping protein subunit alpha, Immunoglobulin J chain, Prosaposin, 78 kDa glucose-regulated protein and Heat shock 70 kDa protein 1A and 1B.Conclusion: All of these proteins are involved in immune system activation, cellular stress responses, proliferation, and invasion thus suggesting their use as biomarkers in patients with NCDs.https://www.frontiersin.org/articles/10.3389/fmolb.2023.1327233/fullsalivaproteomic analysisbiomarkerhealthy subjectsliquid biopsy |
| spellingShingle | P. Dongiovanni M. Meroni Gilda Aiello A. D’Amato N. Cenzato S. Casati G. Damiani C. Fenoglio D. Galimberti D. Galimberti E. Grossi D. Prati G. Lamorte C. Bianco L. Valenti A. Soggiu S. Zapperi C. A. M. La Porta M. Del Fabbro M. Del Fabbro G. M. Tartaglia G. M. Tartaglia Salivary proteomic profile of young healthy subjects Frontiers in Molecular Biosciences saliva proteomic analysis biomarker healthy subjects liquid biopsy |
| title | Salivary proteomic profile of young healthy subjects |
| title_full | Salivary proteomic profile of young healthy subjects |
| title_fullStr | Salivary proteomic profile of young healthy subjects |
| title_full_unstemmed | Salivary proteomic profile of young healthy subjects |
| title_short | Salivary proteomic profile of young healthy subjects |
| title_sort | salivary proteomic profile of young healthy subjects |
| topic | saliva proteomic analysis biomarker healthy subjects liquid biopsy |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1327233/full |
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