Temperature-induced changes in the hydroxy and non-hydroxy fatty acid-containing sphingolipids abundant in the surface membrane of Tetrahymena pyriformis NT-1.
Sphingolipids make up 30 to 40 mole % of the phospholipids found in the surface membrane of Tetrahymena pyriformis NT-1. We have identified the two major classes as non-hydroxy fatty acid-containing ceramide-2-aminoethylphosphonate (NCAEP) and alpha-hydroxy fatty acid-containing ceramide-2-aminoethy...
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1984-01-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520378457 |
| _version_ | 1818941926642024448 |
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| author | K Kaya C S Ramesha G A Thompson, Jr |
| author_facet | K Kaya C S Ramesha G A Thompson, Jr |
| author_sort | K Kaya |
| collection | DOAJ |
| description | Sphingolipids make up 30 to 40 mole % of the phospholipids found in the surface membrane of Tetrahymena pyriformis NT-1. We have identified the two major classes as non-hydroxy fatty acid-containing ceramide-2-aminoethylphosphonate (NCAEP) and alpha-hydroxy fatty acid-containing ceramide-2-aminoethylphosphonate (HCAEP). Both classes were well represented in cells grown at 39 degrees C. At this temperature their principal long chain bases were n-hexadeca-4-sphingenine and n-nonadeca-4-sphingenine. The major fatty acid of NCAEP from 39 degrees C-grown cells was palmitic acid and that of HCAEP was alpha-hydroxypalmitic acid. Cells grown at 15 degrees C contained NCAEP, but only traces of HCAEP. By analyzing the incorporation of [1-14C]palmitic acid into cells growing isothermally or shifted from 15 degrees C to 39 degrees C, we obtained evidence favoring a direct conversion of NCAEP to HCAEP. This conversion was blocked in cells grown at 15 degrees C, causing an accumulation of NCAEP. Tetrahymena is a useful model system for studying the poorly understood alpha-hydroxylation process that is of critical importance in myelination of animal nervous tissues. |
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| format | Article |
| id | doaj.art-f0a7b143da964b209754da3ed088b362 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T07:03:18Z |
| publishDate | 1984-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-f0a7b143da964b209754da3ed088b3622022-12-21T19:49:09ZengElsevierJournal of Lipid Research0022-22751984-01-012516874Temperature-induced changes in the hydroxy and non-hydroxy fatty acid-containing sphingolipids abundant in the surface membrane of Tetrahymena pyriformis NT-1.K KayaC S RameshaG A Thompson, JrSphingolipids make up 30 to 40 mole % of the phospholipids found in the surface membrane of Tetrahymena pyriformis NT-1. We have identified the two major classes as non-hydroxy fatty acid-containing ceramide-2-aminoethylphosphonate (NCAEP) and alpha-hydroxy fatty acid-containing ceramide-2-aminoethylphosphonate (HCAEP). Both classes were well represented in cells grown at 39 degrees C. At this temperature their principal long chain bases were n-hexadeca-4-sphingenine and n-nonadeca-4-sphingenine. The major fatty acid of NCAEP from 39 degrees C-grown cells was palmitic acid and that of HCAEP was alpha-hydroxypalmitic acid. Cells grown at 15 degrees C contained NCAEP, but only traces of HCAEP. By analyzing the incorporation of [1-14C]palmitic acid into cells growing isothermally or shifted from 15 degrees C to 39 degrees C, we obtained evidence favoring a direct conversion of NCAEP to HCAEP. This conversion was blocked in cells grown at 15 degrees C, causing an accumulation of NCAEP. Tetrahymena is a useful model system for studying the poorly understood alpha-hydroxylation process that is of critical importance in myelination of animal nervous tissues.http://www.sciencedirect.com/science/article/pii/S0022227520378457 |
| spellingShingle | K Kaya C S Ramesha G A Thompson, Jr Temperature-induced changes in the hydroxy and non-hydroxy fatty acid-containing sphingolipids abundant in the surface membrane of Tetrahymena pyriformis NT-1. Journal of Lipid Research |
| title | Temperature-induced changes in the hydroxy and non-hydroxy fatty acid-containing sphingolipids abundant in the surface membrane of Tetrahymena pyriformis NT-1. |
| title_full | Temperature-induced changes in the hydroxy and non-hydroxy fatty acid-containing sphingolipids abundant in the surface membrane of Tetrahymena pyriformis NT-1. |
| title_fullStr | Temperature-induced changes in the hydroxy and non-hydroxy fatty acid-containing sphingolipids abundant in the surface membrane of Tetrahymena pyriformis NT-1. |
| title_full_unstemmed | Temperature-induced changes in the hydroxy and non-hydroxy fatty acid-containing sphingolipids abundant in the surface membrane of Tetrahymena pyriformis NT-1. |
| title_short | Temperature-induced changes in the hydroxy and non-hydroxy fatty acid-containing sphingolipids abundant in the surface membrane of Tetrahymena pyriformis NT-1. |
| title_sort | temperature induced changes in the hydroxy and non hydroxy fatty acid containing sphingolipids abundant in the surface membrane of tetrahymena pyriformis nt 1 |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520378457 |
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