Immunolocalization of Cd34 Positive Progenitor Cells in Diabetic and Non Diabetic Periodontitis Patients – A Comparative Study
Background: Little research has been documented to determine the CD34 positive cells in healthy periodontium, chronic periodontitis and in chronic periodontitis with diabetes mellitus individuals. Aim: The aim of the present study was to evaluate and compare the CD34 positive progenitor cells o...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2014-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/5191/9827_CE(Ra)_F(Sh)_PF1(PU_Sh)_PFA(Sh)_PF2(PAG)_PFA2(PAG).pdf |
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| author | Sathyanarayana Baddam Gautami S Penmetsa Narendra Mandalapu Sitaramaraju DV Ravikanth Mannem Rama Krishna Alla Praveen Gadde |
| author_facet | Sathyanarayana Baddam Gautami S Penmetsa Narendra Mandalapu Sitaramaraju DV Ravikanth Mannem Rama Krishna Alla Praveen Gadde |
| author_sort | Sathyanarayana Baddam |
| collection | DOAJ |
| description | Background: Little research has been documented to
determine the CD34 positive cells in healthy periodontium,
chronic periodontitis and in chronic periodontitis with diabetes
mellitus individuals.
Aim: The aim of the present study was to evaluate and compare
the CD34 positive progenitor cells of the gingiva in patients
with healthy periodontium, chronic periodontitis and chronic
periodontitis with Diabetes Mellitus.
Materials and Methods: A total number of 75 patients
were divided into 3 groups which included Group I (healthy
periodontium), Group II (chronic periodontitis) and Group
III (chronic periodontitis with diabetes mellitus). Periodontal
examination included Plaque index, Gingival index, Gingival
bleeding index, Probing pocket depth and Clinical attachment
levels. Gingival biopsies were collected from each participant
and they were fixed in formalin embedded in paraffin which
was later subjected to immunohistochemical procedure with
anti-CD34 (a stemness marker). T-Test and Regression analysis
(R-square) was used to analyse the data.
Results: The mean number of CD34 positive cells were higher
in group III (5.71±1.97) compared to Group chronic periodontitis
group I (4.98± 2.08) and II (4.48± 1.24).
Conclusion: Although CD34 is a non specific stemness marker,
results suggest that there is a significant difference in the
number of CD34 positive progenitor cells between Group II and
Group III but no significant difference was observed between
Group I, II and Group I, III. |
| first_indexed | 2024-12-12T20:13:30Z |
| format | Article |
| id | doaj.art-f0a808126f7f43128c8f30bbdfbdc535 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-12T20:13:30Z |
| publishDate | 2014-11-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-f0a808126f7f43128c8f30bbdfbdc5352022-12-22T00:13:27ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-11-01811ZC96ZC9910.7860/JCDR/2014/9827.5191Immunolocalization of Cd34 Positive Progenitor Cells in Diabetic and Non Diabetic Periodontitis Patients – A Comparative StudySathyanarayana Baddam0Gautami S Penmetsa1Narendra Mandalapu2Sitaramaraju DV3Ravikanth Mannem4Rama Krishna Alla5Praveen Gadde6Senior Lecturer, Department of Periodontics, CKS Teja Institute of Dental Sciences and Research, Thirupathi, India.Professor, Department of Periodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India. Reader, Department of Conservative Dentistry & Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Postgraduate Student, Department of Periodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Associate Professor, Department of Oral Pathology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Assistant Professor, Department of Dental Materials, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Senior Lecturer, Department of Public Health Dentistry, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.Background: Little research has been documented to determine the CD34 positive cells in healthy periodontium, chronic periodontitis and in chronic periodontitis with diabetes mellitus individuals. Aim: The aim of the present study was to evaluate and compare the CD34 positive progenitor cells of the gingiva in patients with healthy periodontium, chronic periodontitis and chronic periodontitis with Diabetes Mellitus. Materials and Methods: A total number of 75 patients were divided into 3 groups which included Group I (healthy periodontium), Group II (chronic periodontitis) and Group III (chronic periodontitis with diabetes mellitus). Periodontal examination included Plaque index, Gingival index, Gingival bleeding index, Probing pocket depth and Clinical attachment levels. Gingival biopsies were collected from each participant and they were fixed in formalin embedded in paraffin which was later subjected to immunohistochemical procedure with anti-CD34 (a stemness marker). T-Test and Regression analysis (R-square) was used to analyse the data. Results: The mean number of CD34 positive cells were higher in group III (5.71±1.97) compared to Group chronic periodontitis group I (4.98± 2.08) and II (4.48± 1.24). Conclusion: Although CD34 is a non specific stemness marker, results suggest that there is a significant difference in the number of CD34 positive progenitor cells between Group II and Group III but no significant difference was observed between Group I, II and Group I, III.https://jcdr.net/articles/PDF/5191/9827_CE(Ra)_F(Sh)_PF1(PU_Sh)_PFA(Sh)_PF2(PAG)_PFA2(PAG).pdfcd34chronic periodontitisdiabetes mellitusmesenchymal progenitor cellsparavascular and stromal progenitor cells |
| spellingShingle | Sathyanarayana Baddam Gautami S Penmetsa Narendra Mandalapu Sitaramaraju DV Ravikanth Mannem Rama Krishna Alla Praveen Gadde Immunolocalization of Cd34 Positive Progenitor Cells in Diabetic and Non Diabetic Periodontitis Patients – A Comparative Study Journal of Clinical and Diagnostic Research cd34 chronic periodontitis diabetes mellitus mesenchymal progenitor cells paravascular and stromal progenitor cells |
| title | Immunolocalization of Cd34 Positive Progenitor Cells in Diabetic and Non Diabetic Periodontitis Patients – A Comparative Study |
| title_full | Immunolocalization of Cd34 Positive Progenitor Cells in Diabetic and Non Diabetic Periodontitis Patients – A Comparative Study |
| title_fullStr | Immunolocalization of Cd34 Positive Progenitor Cells in Diabetic and Non Diabetic Periodontitis Patients – A Comparative Study |
| title_full_unstemmed | Immunolocalization of Cd34 Positive Progenitor Cells in Diabetic and Non Diabetic Periodontitis Patients – A Comparative Study |
| title_short | Immunolocalization of Cd34 Positive Progenitor Cells in Diabetic and Non Diabetic Periodontitis Patients – A Comparative Study |
| title_sort | immunolocalization of cd34 positive progenitor cells in diabetic and non diabetic periodontitis patients a comparative study |
| topic | cd34 chronic periodontitis diabetes mellitus mesenchymal progenitor cells paravascular and stromal progenitor cells |
| url | https://jcdr.net/articles/PDF/5191/9827_CE(Ra)_F(Sh)_PF1(PU_Sh)_PFA(Sh)_PF2(PAG)_PFA2(PAG).pdf |
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