Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval <it>Drosophila</it>
<p>Abstract</p> <p>Background</p> <p>The development, morphology and genetics of sensory neurons have been extensively studied in <it>Drosophila</it>. Sensory neurons in the body wall of larval <it>Drosophila </it>in particular have been the subj...
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| Format: | Article |
| Language: | English |
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BMC
2010-06-01
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| Series: | BMC Research Notes |
| Online Access: | http://www.biomedcentral.com/1756-0500/3/154 |
| _version_ | 1828419654595379200 |
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| author | Bate Michael Nair Amit Pulver Stefan R |
| author_facet | Bate Michael Nair Amit Pulver Stefan R |
| author_sort | Bate Michael |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The development, morphology and genetics of sensory neurons have been extensively studied in <it>Drosophila</it>. Sensory neurons in the body wall of larval <it>Drosophila </it>in particular have been the subject of numerous anatomical studies, however, little is known about the intrinsic electrical properties of larval sensory cells.</p> <p>Findings</p> <p>We performed whole cell patch recordings from an identified peripheral sensory cell, the dorsal bipolar sensory neuron (dbd) and measured voltage-gated ionic currents in 1<sup>st </sup>instar larvae. Voltage clamp analysis revealed that dbds have a TEA sensitive, non-inactivating <it>I</it><sub><it>K </it></sub>type potassium current as well as a 4-AP sensitive, inactivating <it>I</it><sub><it>A </it></sub>type potassium current. dbds also show a voltage-gated calcium current (<it>I</it><sub><it>Ca</it></sub>) and a voltage-gated sodium current (<it>I</it><sub><it>Na</it></sub>).</p> <p>Conclusions</p> <p>This work provides a first characterization of voltage-activated ionic currents in an identified body-wall sensory neuron in larval <it>Drosophila</it>. Overall, we establish baseline physiology data for future studies aimed at understanding the ionic and genetic basis of sensory neuron function in fruit flies and other model organisms.</p> |
| first_indexed | 2024-12-10T14:57:42Z |
| format | Article |
| id | doaj.art-f0aa579b1ad14429abcce9b5bde2a547 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-10T14:57:42Z |
| publishDate | 2010-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-f0aa579b1ad14429abcce9b5bde2a5472022-12-22T01:44:15ZengBMCBMC Research Notes1756-05002010-06-013115410.1186/1756-0500-3-154Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval <it>Drosophila</it>Bate MichaelNair AmitPulver Stefan R<p>Abstract</p> <p>Background</p> <p>The development, morphology and genetics of sensory neurons have been extensively studied in <it>Drosophila</it>. Sensory neurons in the body wall of larval <it>Drosophila </it>in particular have been the subject of numerous anatomical studies, however, little is known about the intrinsic electrical properties of larval sensory cells.</p> <p>Findings</p> <p>We performed whole cell patch recordings from an identified peripheral sensory cell, the dorsal bipolar sensory neuron (dbd) and measured voltage-gated ionic currents in 1<sup>st </sup>instar larvae. Voltage clamp analysis revealed that dbds have a TEA sensitive, non-inactivating <it>I</it><sub><it>K </it></sub>type potassium current as well as a 4-AP sensitive, inactivating <it>I</it><sub><it>A </it></sub>type potassium current. dbds also show a voltage-gated calcium current (<it>I</it><sub><it>Ca</it></sub>) and a voltage-gated sodium current (<it>I</it><sub><it>Na</it></sub>).</p> <p>Conclusions</p> <p>This work provides a first characterization of voltage-activated ionic currents in an identified body-wall sensory neuron in larval <it>Drosophila</it>. Overall, we establish baseline physiology data for future studies aimed at understanding the ionic and genetic basis of sensory neuron function in fruit flies and other model organisms.</p>http://www.biomedcentral.com/1756-0500/3/154 |
| spellingShingle | Bate Michael Nair Amit Pulver Stefan R Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval <it>Drosophila</it> BMC Research Notes |
| title | Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval <it>Drosophila</it> |
| title_full | Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval <it>Drosophila</it> |
| title_fullStr | Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval <it>Drosophila</it> |
| title_full_unstemmed | Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval <it>Drosophila</it> |
| title_short | Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval <it>Drosophila</it> |
| title_sort | characterization of voltage gated ionic currents in a peripheral sensory neuron in larval it drosophila it |
| url | http://www.biomedcentral.com/1756-0500/3/154 |
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