Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell Patterning
This paper describes a light-addressable electrolytic system used to perform an electrodeposition of magnetically-guided cells encapsulated in alginate hydrogels using a digital micromirror device (DMD) for three-dimensional cell patterning. In this system, the magnetically-labeled cells were first...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-11-01
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| Series: | Micromachines |
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| Online Access: | http://www.mdpi.com/2072-666X/5/4/1173 |
| _version_ | 1818320223799869440 |
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| author | Shih-Hao Huang Hsiao-Tzu Chu Yan-Min Liou Kuo-Sheng Huang |
| author_facet | Shih-Hao Huang Hsiao-Tzu Chu Yan-Min Liou Kuo-Sheng Huang |
| author_sort | Shih-Hao Huang |
| collection | DOAJ |
| description | This paper describes a light-addressable electrolytic system used to perform an electrodeposition of magnetically-guided cells encapsulated in alginate hydrogels using a digital micromirror device (DMD) for three-dimensional cell patterning. In this system, the magnetically-labeled cells were first manipulated into a specific arrangement by changing the orientation of the magnetic field, and then a patterned light illumination was projected onto a photoconductive substrate serving as a photo-anode to cause gelation of calcium alginate through sol-gel transition. By controlling the illumination pattern on the DMD, we first successfully produced cell-encapsulated multilayer alginate hydrogels with different shapes and sizes in each layer via performing multiplexed micropatterning. By combining the magnetically-labeled cells, light-addressable electrodeposition, and orientation of the magnetic fields, we have successfully demonstrated to fabricate two layers of the cell-encapsulated alginate hydrogels, where cells in each layer can be manipulated into cross-directional arrangements that mimic natural tissue. Our proposed method provides a programmable method for the spatiotemporally controllable assembly of cell populations into three-dimensional cell patterning and could have a wide range of biological applications in tissue engineering, toxicology, and drug discovery. |
| first_indexed | 2024-12-13T10:21:36Z |
| format | Article |
| id | doaj.art-f0abe9b5ddaa4854acba645590bd199c |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-13T10:21:36Z |
| publishDate | 2014-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-f0abe9b5ddaa4854acba645590bd199c2022-12-21T23:51:11ZengMDPI AGMicromachines2072-666X2014-11-01541173118710.3390/mi5041173mi5041173Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell PatterningShih-Hao Huang0Hsiao-Tzu Chu1Yan-Min Liou2Kuo-Sheng Huang3Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung 202-24, TaiwanDepartment of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung 202-24, TaiwanDepartment of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung 202-24, TaiwanDepartment of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung 202-24, TaiwanThis paper describes a light-addressable electrolytic system used to perform an electrodeposition of magnetically-guided cells encapsulated in alginate hydrogels using a digital micromirror device (DMD) for three-dimensional cell patterning. In this system, the magnetically-labeled cells were first manipulated into a specific arrangement by changing the orientation of the magnetic field, and then a patterned light illumination was projected onto a photoconductive substrate serving as a photo-anode to cause gelation of calcium alginate through sol-gel transition. By controlling the illumination pattern on the DMD, we first successfully produced cell-encapsulated multilayer alginate hydrogels with different shapes and sizes in each layer via performing multiplexed micropatterning. By combining the magnetically-labeled cells, light-addressable electrodeposition, and orientation of the magnetic fields, we have successfully demonstrated to fabricate two layers of the cell-encapsulated alginate hydrogels, where cells in each layer can be manipulated into cross-directional arrangements that mimic natural tissue. Our proposed method provides a programmable method for the spatiotemporally controllable assembly of cell populations into three-dimensional cell patterning and could have a wide range of biological applications in tissue engineering, toxicology, and drug discovery.http://www.mdpi.com/2072-666X/5/4/1173electrodepositiondigital micromirror devicecalcium alginate |
| spellingShingle | Shih-Hao Huang Hsiao-Tzu Chu Yan-Min Liou Kuo-Sheng Huang Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell Patterning Micromachines electrodeposition digital micromirror device calcium alginate |
| title | Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell Patterning |
| title_full | Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell Patterning |
| title_fullStr | Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell Patterning |
| title_full_unstemmed | Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell Patterning |
| title_short | Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell Patterning |
| title_sort | light addressable electrodeposition of magnetically guided cells encapsulated in alginate hydrogels for three dimensional cell patterning |
| topic | electrodeposition digital micromirror device calcium alginate |
| url | http://www.mdpi.com/2072-666X/5/4/1173 |
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