Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in <i>Dendroctonus armandi</i>
The short neuropeptide F (sNPF) is an essential signaling molecule that is evolutionarily conserved and involved in a broad range of physiological functions in the invertebrates, by interacting with sNPF receptors, which belong to G protein-coupled receptors (GPCR). However, the function of sNPF in...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/9/844 |
| _version_ | 1797518776018665472 |
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| author | Bin Liu Danyang Fu Hang Ning Ming Tang Hui Chen |
| author_facet | Bin Liu Danyang Fu Hang Ning Ming Tang Hui Chen |
| author_sort | Bin Liu |
| collection | DOAJ |
| description | The short neuropeptide F (sNPF) is an essential signaling molecule that is evolutionarily conserved and involved in a broad range of physiological functions in the invertebrates, by interacting with sNPF receptors, which belong to G protein-coupled receptors (GPCR). However, the function of sNPF in regulating the food intake of <i>Dendroctonus armandi</i> has been unclear. In this study, we cloned and characterized cDNAs encoding sNPF and sNPF receptor in the <i>D. armandi</i> and made bioinformatics predictions on the deduced amino acid sequences. They had a high degree of similarity to that of <i>Dendroctonus ponderosa.</i> Quantitative real-time reverse transcription PCR (qRT-PCR) revealed that the transcript levels of both sNPF and sNPFR varied across developmental stages and body parts. In addition, the sNPF and sNPFR expression levels were upregulated in starved beetles, and the expression levels recovered after re-feeding. Furthermore, RNAi knockdown by the injection of sNPF and sNPFR dsRNA into beetles significantly increased mortality and reduced their food intake and body weight, and also caused decrease of glycogen and free fatty acid and increase of trehalose. These results indicate that sNPF signaling pathway plays an important role in the regulation of food intake and provides a potential molecular target for the eco-friendly control strategies of this pest. |
| first_indexed | 2024-03-10T07:34:22Z |
| format | Article |
| id | doaj.art-f0ae21cc90b345a6bb6b54c5c2559a55 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T07:34:22Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-f0ae21cc90b345a6bb6b54c5c2559a552023-11-22T13:37:25ZengMDPI AGInsects2075-44502021-09-0112984410.3390/insects12090844Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in <i>Dendroctonus armandi</i>Bin Liu0Danyang Fu1Hang Ning2Ming Tang3Hui Chen4College of Forestry, Northwest A&F University, Xianyang 712100, ChinaCollege of Forestry, Northwest A&F University, Xianyang 712100, ChinaCollege of Forestry, Northwest A&F University, Xianyang 712100, ChinaCollege of Forestry, Northwest A&F University, Xianyang 712100, ChinaCollege of Forestry, Northwest A&F University, Xianyang 712100, ChinaThe short neuropeptide F (sNPF) is an essential signaling molecule that is evolutionarily conserved and involved in a broad range of physiological functions in the invertebrates, by interacting with sNPF receptors, which belong to G protein-coupled receptors (GPCR). However, the function of sNPF in regulating the food intake of <i>Dendroctonus armandi</i> has been unclear. In this study, we cloned and characterized cDNAs encoding sNPF and sNPF receptor in the <i>D. armandi</i> and made bioinformatics predictions on the deduced amino acid sequences. They had a high degree of similarity to that of <i>Dendroctonus ponderosa.</i> Quantitative real-time reverse transcription PCR (qRT-PCR) revealed that the transcript levels of both sNPF and sNPFR varied across developmental stages and body parts. In addition, the sNPF and sNPFR expression levels were upregulated in starved beetles, and the expression levels recovered after re-feeding. Furthermore, RNAi knockdown by the injection of sNPF and sNPFR dsRNA into beetles significantly increased mortality and reduced their food intake and body weight, and also caused decrease of glycogen and free fatty acid and increase of trehalose. These results indicate that sNPF signaling pathway plays an important role in the regulation of food intake and provides a potential molecular target for the eco-friendly control strategies of this pest.https://www.mdpi.com/2075-4450/12/9/844<i>Dendroctonus armandi</i>short neuropeptide Fshort neuropeptide F receptorfood intakemolecular targetRNA interference |
| spellingShingle | Bin Liu Danyang Fu Hang Ning Ming Tang Hui Chen Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in <i>Dendroctonus armandi</i> Insects <i>Dendroctonus armandi</i> short neuropeptide F short neuropeptide F receptor food intake molecular target RNA interference |
| title | Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in <i>Dendroctonus armandi</i> |
| title_full | Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in <i>Dendroctonus armandi</i> |
| title_fullStr | Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in <i>Dendroctonus armandi</i> |
| title_full_unstemmed | Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in <i>Dendroctonus armandi</i> |
| title_short | Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in <i>Dendroctonus armandi</i> |
| title_sort | identification of the short neuropeptide f and short neuropeptide f receptor genes and their roles of food intake in i dendroctonus armandi i |
| topic | <i>Dendroctonus armandi</i> short neuropeptide F short neuropeptide F receptor food intake molecular target RNA interference |
| url | https://www.mdpi.com/2075-4450/12/9/844 |
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