α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium
Microctis Folium, the leaves of Microcos paniculata L., is a commonly used herbal tea material. The methanol extract of Microctis Folium and its principle compounds vitexin (1), isovitexin (2) and isorhamnetin 3-O-β-D-rutinoside (3) were investigated for their α-glucosidase inhibitory effects. The e...
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MDPI AG
2013-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/18/4/4221 |
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| author | Wen-Cai Ye Ying Wang Xian-Tao Zhang Xiao-Qi Zhang Ru Yan Peng Li Ping Li Yan-Gan Chen Qing-Wen Zhang |
| author_facet | Wen-Cai Ye Ying Wang Xian-Tao Zhang Xiao-Qi Zhang Ru Yan Peng Li Ping Li Yan-Gan Chen Qing-Wen Zhang |
| author_sort | Wen-Cai Ye |
| collection | DOAJ |
| description | Microctis Folium, the leaves of Microcos paniculata L., is a commonly used herbal tea material. The methanol extract of Microctis Folium and its principle compounds vitexin (1), isovitexin (2) and isorhamnetin 3-O-β-D-rutinoside (3) were investigated for their α-glucosidase inhibitory effects. The extract showed strong α-glucosidase inhibitory effect (IC50 = 61.30 μg/mL) and the three flavonoid glycosides 1–3 exerted satisfactory α-glucosidase inhibitory effects, with IC50 values of 244.0 μM, 266.2 μM and 275.4 μM, respectively. A simple and reliable HPLC-DAD method was developed for the quantification of the three flavonoid glycosides in Microctis Folium and applied successfully to determine contents of these components in samples collected from different locations. This study suggested that Microctis Folium may be a promising candidate for development of herbal antidiabetes drugs, and vitexin, isovitexin and isorhamnetin 3-O-β-D-rutinoside can be the biomarkers and chemical markers for this plant substance. |
| first_indexed | 2024-12-19T19:34:08Z |
| format | Article |
| id | doaj.art-f0afc25c444240e48cadf7baf069eb3d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-19T19:34:08Z |
| publishDate | 2013-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f0afc25c444240e48cadf7baf069eb3d2022-12-21T20:08:32ZengMDPI AGMolecules1420-30492013-04-011844221423210.3390/molecules18044221α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis foliumWen-Cai YeYing WangXian-Tao ZhangXiao-Qi ZhangRu YanPeng LiPing LiYan-Gan ChenQing-Wen ZhangMicroctis Folium, the leaves of Microcos paniculata L., is a commonly used herbal tea material. The methanol extract of Microctis Folium and its principle compounds vitexin (1), isovitexin (2) and isorhamnetin 3-O-β-D-rutinoside (3) were investigated for their α-glucosidase inhibitory effects. The extract showed strong α-glucosidase inhibitory effect (IC50 = 61.30 μg/mL) and the three flavonoid glycosides 1–3 exerted satisfactory α-glucosidase inhibitory effects, with IC50 values of 244.0 μM, 266.2 μM and 275.4 μM, respectively. A simple and reliable HPLC-DAD method was developed for the quantification of the three flavonoid glycosides in Microctis Folium and applied successfully to determine contents of these components in samples collected from different locations. This study suggested that Microctis Folium may be a promising candidate for development of herbal antidiabetes drugs, and vitexin, isovitexin and isorhamnetin 3-O-β-D-rutinoside can be the biomarkers and chemical markers for this plant substance.http://www.mdpi.com/1420-3049/18/4/4221Microctis Foliumflavonoid glycosidesα-glucosidase inhibitory effectHPLC-DADquantification |
| spellingShingle | Wen-Cai Ye Ying Wang Xian-Tao Zhang Xiao-Qi Zhang Ru Yan Peng Li Ping Li Yan-Gan Chen Qing-Wen Zhang α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium Molecules Microctis Folium flavonoid glycosides α-glucosidase inhibitory effect HPLC-DAD quantification |
| title | α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium |
| title_full | α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium |
| title_fullStr | α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium |
| title_full_unstemmed | α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium |
| title_short | α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium |
| title_sort | α glucosidase inhibitory effect and simultaneous quantification of three major flavonoid glycosides in microctis folium |
| topic | Microctis Folium flavonoid glycosides α-glucosidase inhibitory effect HPLC-DAD quantification |
| url | http://www.mdpi.com/1420-3049/18/4/4221 |
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