Characterising the Response of Human Breast Cancer Cells to Polyamine Modulation
Breast cancer is a complex heterogeneous disease with multiple underlying causes. The polyamines putrescine, spermidine, and spermine are polycationic molecules essential for cell proliferation. Their biosynthesis is upregulated in breast cancer and they contribute to disease progression. While elev...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/5/743 |
| _version_ | 1797533852148695040 |
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| author | Oluwaseun Akinyele Heather M. Wallace |
| author_facet | Oluwaseun Akinyele Heather M. Wallace |
| author_sort | Oluwaseun Akinyele |
| collection | DOAJ |
| description | Breast cancer is a complex heterogeneous disease with multiple underlying causes. The polyamines putrescine, spermidine, and spermine are polycationic molecules essential for cell proliferation. Their biosynthesis is upregulated in breast cancer and they contribute to disease progression. While elevated polyamines are linked to breast cancer cell proliferation, there is little evidence to suggest breast cancer cells of different hormone receptor status are equally dependent on polyamines. In this study, we characterized the responses of two breast cancer cells, ER+ (oestrogen receptor positive) MCF-7 and ER- MDA-MB-231 cell lines, to polyamine modulation and determined the requirement of each polyamine for cancer cell growth. The cells were exposed to DFMO (a polyamine pathway inhibitor) at various concentrations under different conditions, after which several growth parameters were determined. Exposure of both cell lines to DFMO induced differential growth responses, MCF-7 cells showed greater sensitivity to polyamine pathway inhibition at various DFMO concentrations than the MDA-MB-231 cells. Analysis of intracellular DFMO after withdrawal from growth medium showed residual DFMO in the cells with concomitant decreases in polyamine content, ODC protein level, and cell growth. Addition of exogenous polyamines reversed the cell growth inhibition, and this growth recovery appears to be partly dependent on the spermidine content of the cell. Similarly, DFMO exposure inhibits the global translation state of the cells, with spermidine addition reversing the inhibition of translation in the breast cancer cells. Taken together, these data suggest that breast cancer cells are differentially sensitive to the antitumour effects of polyamine depletion, thus, targeting polyamine metabolism might be therapeutically beneficial in breast cancer management based on their subtype. |
| first_indexed | 2024-03-10T11:21:31Z |
| format | Article |
| id | doaj.art-f0b037bf2eb64eaaac07393dfed19ccf |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T11:21:31Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-f0b037bf2eb64eaaac07393dfed19ccf2023-11-21T20:02:25ZengMDPI AGBiomolecules2218-273X2021-05-0111574310.3390/biom11050743Characterising the Response of Human Breast Cancer Cells to Polyamine ModulationOluwaseun Akinyele0Heather M. Wallace1Institute of Medical Sciences, School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen AB25 2ZD, UKInstitute of Medical Sciences, School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen AB25 2ZD, UKBreast cancer is a complex heterogeneous disease with multiple underlying causes. The polyamines putrescine, spermidine, and spermine are polycationic molecules essential for cell proliferation. Their biosynthesis is upregulated in breast cancer and they contribute to disease progression. While elevated polyamines are linked to breast cancer cell proliferation, there is little evidence to suggest breast cancer cells of different hormone receptor status are equally dependent on polyamines. In this study, we characterized the responses of two breast cancer cells, ER+ (oestrogen receptor positive) MCF-7 and ER- MDA-MB-231 cell lines, to polyamine modulation and determined the requirement of each polyamine for cancer cell growth. The cells were exposed to DFMO (a polyamine pathway inhibitor) at various concentrations under different conditions, after which several growth parameters were determined. Exposure of both cell lines to DFMO induced differential growth responses, MCF-7 cells showed greater sensitivity to polyamine pathway inhibition at various DFMO concentrations than the MDA-MB-231 cells. Analysis of intracellular DFMO after withdrawal from growth medium showed residual DFMO in the cells with concomitant decreases in polyamine content, ODC protein level, and cell growth. Addition of exogenous polyamines reversed the cell growth inhibition, and this growth recovery appears to be partly dependent on the spermidine content of the cell. Similarly, DFMO exposure inhibits the global translation state of the cells, with spermidine addition reversing the inhibition of translation in the breast cancer cells. Taken together, these data suggest that breast cancer cells are differentially sensitive to the antitumour effects of polyamine depletion, thus, targeting polyamine metabolism might be therapeutically beneficial in breast cancer management based on their subtype.https://www.mdpi.com/2218-273X/11/5/743polyaminescell growthbreast cancer subtypesDFMO |
| spellingShingle | Oluwaseun Akinyele Heather M. Wallace Characterising the Response of Human Breast Cancer Cells to Polyamine Modulation Biomolecules polyamines cell growth breast cancer subtypes DFMO |
| title | Characterising the Response of Human Breast Cancer Cells to Polyamine Modulation |
| title_full | Characterising the Response of Human Breast Cancer Cells to Polyamine Modulation |
| title_fullStr | Characterising the Response of Human Breast Cancer Cells to Polyamine Modulation |
| title_full_unstemmed | Characterising the Response of Human Breast Cancer Cells to Polyamine Modulation |
| title_short | Characterising the Response of Human Breast Cancer Cells to Polyamine Modulation |
| title_sort | characterising the response of human breast cancer cells to polyamine modulation |
| topic | polyamines cell growth breast cancer subtypes DFMO |
| url | https://www.mdpi.com/2218-273X/11/5/743 |
| work_keys_str_mv | AT oluwaseunakinyele characterisingtheresponseofhumanbreastcancercellstopolyaminemodulation AT heathermwallace characterisingtheresponseofhumanbreastcancercellstopolyaminemodulation |