Simultaneous host and parasite expression profiling identifies tissue-specific transcriptional programs associated with susceptibility or resistance to experimental cerebral malaria
<p>Abstract</p> <p>Background</p> <p>The development and outcome of cerebral malaria (CM) reflects a complex interplay between parasite-expressed virulence factors and host response to infection. The murine CM model, <it>Plasmodium berghei </it>ANKA (PbA), w...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2006-11-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/7/295 |
| _version_ | 1819123691589468160 |
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| author | Liles W Conrad Mohammad Naveed Peña-Castillo Lourdes Lovegrove Fiona E Hughes Timothy R Kain Kevin C |
| author_facet | Liles W Conrad Mohammad Naveed Peña-Castillo Lourdes Lovegrove Fiona E Hughes Timothy R Kain Kevin C |
| author_sort | Liles W Conrad |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The development and outcome of cerebral malaria (CM) reflects a complex interplay between parasite-expressed virulence factors and host response to infection. The murine CM model, <it>Plasmodium berghei </it>ANKA (PbA), which simulates many of the features of human CM, provides an excellent system to study this host/parasite interface. We designed "combination" microarrays that concurrently detect genome-wide transcripts of both PbA and mouse, and examined parasite and host transcriptional programs during infection of CM-susceptible (C57BL/6) and CM-resistant (BALB/c) mice.</p> <p>Results</p> <p>Analysis of expression data from brain, lung, liver, and spleen of PbA infected mice showed that both host and parasite gene expression can be examined using a single microarray, and parasite transcripts can be detected within whole organs at a time when peripheral blood parasitemia is low. Parasites display a unique transcriptional signature in each tissue, and lung appears to be a large reservoir for metabolically active parasites. In comparisons of susceptible versus resistant animals, both host and parasite display distinct, organ-specific transcriptional profiles. Differentially expressed mouse genes were related to humoral immune response, complement activation, or cell-cell interactions. PbA displayed differential expression of genes related to biosynthetic activities.</p> <p>Conclusion</p> <p>These data show that host and parasite gene expression profiles can be simultaneously analysed using a single "combination" microarray, and that both the mouse and malaria parasite display distinct tissue- and strain-specific responses during infection. This technology facilitates the dissection of host-pathogen interactions in experimental cerebral malaria and could be extended to other disease models.</p> |
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| format | Article |
| id | doaj.art-f0b6be3702854e89aecbb3b0e119391a |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-22T07:12:23Z |
| publishDate | 2006-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-f0b6be3702854e89aecbb3b0e119391a2022-12-21T18:34:29ZengBMCBMC Genomics1471-21642006-11-017129510.1186/1471-2164-7-295Simultaneous host and parasite expression profiling identifies tissue-specific transcriptional programs associated with susceptibility or resistance to experimental cerebral malariaLiles W ConradMohammad NaveedPeña-Castillo LourdesLovegrove Fiona EHughes Timothy RKain Kevin C<p>Abstract</p> <p>Background</p> <p>The development and outcome of cerebral malaria (CM) reflects a complex interplay between parasite-expressed virulence factors and host response to infection. The murine CM model, <it>Plasmodium berghei </it>ANKA (PbA), which simulates many of the features of human CM, provides an excellent system to study this host/parasite interface. We designed "combination" microarrays that concurrently detect genome-wide transcripts of both PbA and mouse, and examined parasite and host transcriptional programs during infection of CM-susceptible (C57BL/6) and CM-resistant (BALB/c) mice.</p> <p>Results</p> <p>Analysis of expression data from brain, lung, liver, and spleen of PbA infected mice showed that both host and parasite gene expression can be examined using a single microarray, and parasite transcripts can be detected within whole organs at a time when peripheral blood parasitemia is low. Parasites display a unique transcriptional signature in each tissue, and lung appears to be a large reservoir for metabolically active parasites. In comparisons of susceptible versus resistant animals, both host and parasite display distinct, organ-specific transcriptional profiles. Differentially expressed mouse genes were related to humoral immune response, complement activation, or cell-cell interactions. PbA displayed differential expression of genes related to biosynthetic activities.</p> <p>Conclusion</p> <p>These data show that host and parasite gene expression profiles can be simultaneously analysed using a single "combination" microarray, and that both the mouse and malaria parasite display distinct tissue- and strain-specific responses during infection. This technology facilitates the dissection of host-pathogen interactions in experimental cerebral malaria and could be extended to other disease models.</p>http://www.biomedcentral.com/1471-2164/7/295 |
| spellingShingle | Liles W Conrad Mohammad Naveed Peña-Castillo Lourdes Lovegrove Fiona E Hughes Timothy R Kain Kevin C Simultaneous host and parasite expression profiling identifies tissue-specific transcriptional programs associated with susceptibility or resistance to experimental cerebral malaria BMC Genomics |
| title | Simultaneous host and parasite expression profiling identifies tissue-specific transcriptional programs associated with susceptibility or resistance to experimental cerebral malaria |
| title_full | Simultaneous host and parasite expression profiling identifies tissue-specific transcriptional programs associated with susceptibility or resistance to experimental cerebral malaria |
| title_fullStr | Simultaneous host and parasite expression profiling identifies tissue-specific transcriptional programs associated with susceptibility or resistance to experimental cerebral malaria |
| title_full_unstemmed | Simultaneous host and parasite expression profiling identifies tissue-specific transcriptional programs associated with susceptibility or resistance to experimental cerebral malaria |
| title_short | Simultaneous host and parasite expression profiling identifies tissue-specific transcriptional programs associated with susceptibility or resistance to experimental cerebral malaria |
| title_sort | simultaneous host and parasite expression profiling identifies tissue specific transcriptional programs associated with susceptibility or resistance to experimental cerebral malaria |
| url | http://www.biomedcentral.com/1471-2164/7/295 |
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