Vancomycin sensitivity of methicillin resistant Staphylococcus aureus (MRSA) isolates collected from clinical specimens of Tabriz Imam Reza and Sina hospitals by E-test method, agar screening plate and PCR
Background and Aim: Vancomycin is one of the last drugs used by human for treatment of infections caused by Methicillin resistant Staphylococcus aureus (MRSA) isolates. Since Staphylococcus aureus with heterogeneous, intermediate and complete resistance to vancomycin are in relation to high mortalit...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Farname
2016-05-01
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| Series: | Iranian Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://ijmm.ir/article-1-378-en.html |
| _version_ | 1818624181341782016 |
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| author | Peyman Bohlouli Mohammad Reza Nahaei Safar Farajnia Mojtaba Varshochi Morteza Ghojazadeh Mohammad Akbari Dibavar Firoozeh Safaeeyan |
| author_facet | Peyman Bohlouli Mohammad Reza Nahaei Safar Farajnia Mojtaba Varshochi Morteza Ghojazadeh Mohammad Akbari Dibavar Firoozeh Safaeeyan |
| author_sort | Peyman Bohlouli |
| collection | DOAJ |
| description | Background and Aim: Vancomycin is one of the last drugs used by human for treatment of infections caused by Methicillin resistant Staphylococcus aureus (MRSA) isolates. Since Staphylococcus aureus with heterogeneous, intermediate and complete resistance to vancomycin are in relation to high mortality rate and are increasing in the world, so investigating the incidence of these isolates is necessary.
Materials and Methods: By using several phenotypic tests such as Gram stain, catalase, coagulase, DNase and mannitol fermentations and by screening resistance to methicillin, 100 MRSA isolates were identified. For identifying resistance to vancomycin, phenotypic tests with the disc diffusion method, determining MIC by E-test method and screening test in BHIA (Brain Heart Infusion Agar) containing vancomycin and molecular PCR test were carried out in order to identify femB, mecA and vanA genes.
Results: Resistance to methicillin was 59%. Ranges of vancomycin MICs were between 0.75 µg/ml to 4 µg/ml. By E-test method 92% of isolates were sensitive and 8% showed heterogeneous resistance to vancomycin. However, screening tests in BHIA, containing 3 μg/ml and 4 μg/ml vancomycin, detected 18% heterogeneous resistance and 4% intermediate resistance to vancomycin, respectively. In screening tests using BHIA, with 6 μg/ml vancomycin no isolate was able to grow. Although all of the isolates contained femB and mecA genes, but none of them contained vanA gene.
Conclusion: E-test alone was not able to identify all S.aureus isolates of heterogeneous resistance and intermediate resistance to vancomycin. As a result, the screening tests also should be used along with MIC tests. |
| first_indexed | 2024-12-16T18:52:52Z |
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| id | doaj.art-f0b6d8128f3c46109241d4839bf5add4 |
| institution | Directory Open Access Journal |
| issn | 1735-8612 2345-4342 |
| language | English |
| last_indexed | 2024-12-16T18:52:52Z |
| publishDate | 2016-05-01 |
| publisher | Farname |
| record_format | Article |
| series | Iranian Journal of Medical Microbiology |
| spelling | doaj.art-f0b6d8128f3c46109241d4839bf5add42022-12-21T22:20:39ZengFarnameIranian Journal of Medical Microbiology1735-86122345-43422016-05-011016675Vancomycin sensitivity of methicillin resistant Staphylococcus aureus (MRSA) isolates collected from clinical specimens of Tabriz Imam Reza and Sina hospitals by E-test method, agar screening plate and PCRPeyman Bohlouli0Mohammad Reza Nahaei1Safar Farajnia2Mojtaba Varshochi3Morteza Ghojazadeh4Mohammad Akbari Dibavar5Firoozeh Safaeeyan6 International Branch of Aras Tabriz University of Medical Sciences, Tabriz, Iran School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Tabriz University of Medical Sciences, Tabriz, Iran Tabriz University of Medical Sciences, Tabriz, Iran School of Medicine,Tabriz University of Medical Sciences,Tabriz,Iran School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Background and Aim: Vancomycin is one of the last drugs used by human for treatment of infections caused by Methicillin resistant Staphylococcus aureus (MRSA) isolates. Since Staphylococcus aureus with heterogeneous, intermediate and complete resistance to vancomycin are in relation to high mortality rate and are increasing in the world, so investigating the incidence of these isolates is necessary. Materials and Methods: By using several phenotypic tests such as Gram stain, catalase, coagulase, DNase and mannitol fermentations and by screening resistance to methicillin, 100 MRSA isolates were identified. For identifying resistance to vancomycin, phenotypic tests with the disc diffusion method, determining MIC by E-test method and screening test in BHIA (Brain Heart Infusion Agar) containing vancomycin and molecular PCR test were carried out in order to identify femB, mecA and vanA genes. Results: Resistance to methicillin was 59%. Ranges of vancomycin MICs were between 0.75 µg/ml to 4 µg/ml. By E-test method 92% of isolates were sensitive and 8% showed heterogeneous resistance to vancomycin. However, screening tests in BHIA, containing 3 μg/ml and 4 μg/ml vancomycin, detected 18% heterogeneous resistance and 4% intermediate resistance to vancomycin, respectively. In screening tests using BHIA, with 6 μg/ml vancomycin no isolate was able to grow. Although all of the isolates contained femB and mecA genes, but none of them contained vanA gene. Conclusion: E-test alone was not able to identify all S.aureus isolates of heterogeneous resistance and intermediate resistance to vancomycin. As a result, the screening tests also should be used along with MIC tests.http://ijmm.ir/article-1-378-en.htmlMethicillin- resistant Staphylococcus aureusVancomycinE-testScreening testPCR |
| spellingShingle | Peyman Bohlouli Mohammad Reza Nahaei Safar Farajnia Mojtaba Varshochi Morteza Ghojazadeh Mohammad Akbari Dibavar Firoozeh Safaeeyan Vancomycin sensitivity of methicillin resistant Staphylococcus aureus (MRSA) isolates collected from clinical specimens of Tabriz Imam Reza and Sina hospitals by E-test method, agar screening plate and PCR Iranian Journal of Medical Microbiology Methicillin- resistant Staphylococcus aureus Vancomycin E-test Screening test PCR |
| title | Vancomycin sensitivity of methicillin resistant Staphylococcus aureus (MRSA) isolates collected from clinical specimens of Tabriz Imam Reza and Sina hospitals by E-test method, agar screening plate and PCR |
| title_full | Vancomycin sensitivity of methicillin resistant Staphylococcus aureus (MRSA) isolates collected from clinical specimens of Tabriz Imam Reza and Sina hospitals by E-test method, agar screening plate and PCR |
| title_fullStr | Vancomycin sensitivity of methicillin resistant Staphylococcus aureus (MRSA) isolates collected from clinical specimens of Tabriz Imam Reza and Sina hospitals by E-test method, agar screening plate and PCR |
| title_full_unstemmed | Vancomycin sensitivity of methicillin resistant Staphylococcus aureus (MRSA) isolates collected from clinical specimens of Tabriz Imam Reza and Sina hospitals by E-test method, agar screening plate and PCR |
| title_short | Vancomycin sensitivity of methicillin resistant Staphylococcus aureus (MRSA) isolates collected from clinical specimens of Tabriz Imam Reza and Sina hospitals by E-test method, agar screening plate and PCR |
| title_sort | vancomycin sensitivity of methicillin resistant staphylococcus aureus mrsa isolates collected from clinical specimens of tabriz imam reza and sina hospitals by e test method agar screening plate and pcr |
| topic | Methicillin- resistant Staphylococcus aureus Vancomycin E-test Screening test PCR |
| url | http://ijmm.ir/article-1-378-en.html |
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