Assessing Glycosphingolipid Profiles in Human Health and Disease Using Non-Imaging MALDI Mass Spectrometry
Glycosphingolipids (GSLs) are a glycolipid subtype which plays vital roles in numerous biological processes, cell–cell interactions, as well as oncogenesis and ontogenesis. They are ubiquitous molecules found mostly in cell membranes. Abnormal expression of GSLs as well as altered molecular structur...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/17/9922 |
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| author | Cristina Ramona Novaconi Robert Onulov Alina Florina Serb Eugen Sisu Nicolae Dinca Mihai-Cosmin Pascariu Marius Georgescu |
| author_facet | Cristina Ramona Novaconi Robert Onulov Alina Florina Serb Eugen Sisu Nicolae Dinca Mihai-Cosmin Pascariu Marius Georgescu |
| author_sort | Cristina Ramona Novaconi |
| collection | DOAJ |
| description | Glycosphingolipids (GSLs) are a glycolipid subtype which plays vital roles in numerous biological processes, cell–cell interactions, as well as oncogenesis and ontogenesis. They are ubiquitous molecules found mostly in cell membranes. Abnormal expression of GSLs as well as altered molecular structure have been linked with progression of cancer and metastasis and are involved in the pathophysiology of neurodegenerative, autoimmune, and infectious diseases as well as inherited enzyme defects—glycosphingolipidoses. Matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) plays a leading role in analyzing and characterizing different GSLs, and thus can help to distinguish altered GSL patterns. This review offers insights into the benefits and limitations when using MALDI MS in this field of lipidomic research, with an emphasis on which are the optimal matrices in analyzing GSLs from different tissues (normal and pathological) as well as highlighting GSLs’ particular profiles in various cell cultures, and normal and pathological human tissues obtained by MALDI non-imaging MS (non-IMS). These findings can have implications in further understanding the role of altered GSL expression in various pathological conditions and could be a target for future therapies. |
| first_indexed | 2024-03-10T23:27:14Z |
| format | Article |
| id | doaj.art-f0b9defb509f43bab7d809abb0f6904e |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T23:27:14Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-f0b9defb509f43bab7d809abb0f6904e2023-11-19T07:53:32ZengMDPI AGApplied Sciences2076-34172023-09-011317992210.3390/app13179922Assessing Glycosphingolipid Profiles in Human Health and Disease Using Non-Imaging MALDI Mass SpectrometryCristina Ramona Novaconi0Robert Onulov1Alina Florina Serb2Eugen Sisu3Nicolae Dinca4Mihai-Cosmin Pascariu5Marius Georgescu6Faculty of Medicine, Victor Babes University of Medicine and Pharmacy Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaFaculty of Medicine, Victor Babes University of Medicine and Pharmacy Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaBiochemistry Discipline, Biochemistry and Pharmacology Department, Victor Babes University of Medicine and Pharmacy Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaBiochemistry Discipline, Biochemistry and Pharmacology Department, Victor Babes University of Medicine and Pharmacy Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaDepartment of Technical and Natural Sciences, Faculty of Food Engineering, Tourism and Environmental Protection, “Aurel Vlaicu” University, 310330 Arad, RomaniaDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, “Vasile Goldiș” Western University, 310414 Arad, RomaniaPhysiology Discipline, Functional Sciences Department, Victor Babes University of Medicine and Pharmacy Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaGlycosphingolipids (GSLs) are a glycolipid subtype which plays vital roles in numerous biological processes, cell–cell interactions, as well as oncogenesis and ontogenesis. They are ubiquitous molecules found mostly in cell membranes. Abnormal expression of GSLs as well as altered molecular structure have been linked with progression of cancer and metastasis and are involved in the pathophysiology of neurodegenerative, autoimmune, and infectious diseases as well as inherited enzyme defects—glycosphingolipidoses. Matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) plays a leading role in analyzing and characterizing different GSLs, and thus can help to distinguish altered GSL patterns. This review offers insights into the benefits and limitations when using MALDI MS in this field of lipidomic research, with an emphasis on which are the optimal matrices in analyzing GSLs from different tissues (normal and pathological) as well as highlighting GSLs’ particular profiles in various cell cultures, and normal and pathological human tissues obtained by MALDI non-imaging MS (non-IMS). These findings can have implications in further understanding the role of altered GSL expression in various pathological conditions and could be a target for future therapies.https://www.mdpi.com/2076-3417/13/17/9922glycosphingolipids (GSLs)MALDImass spectrometry (MS)healthy human tissuespathological human tissuescell cultures |
| spellingShingle | Cristina Ramona Novaconi Robert Onulov Alina Florina Serb Eugen Sisu Nicolae Dinca Mihai-Cosmin Pascariu Marius Georgescu Assessing Glycosphingolipid Profiles in Human Health and Disease Using Non-Imaging MALDI Mass Spectrometry Applied Sciences glycosphingolipids (GSLs) MALDI mass spectrometry (MS) healthy human tissues pathological human tissues cell cultures |
| title | Assessing Glycosphingolipid Profiles in Human Health and Disease Using Non-Imaging MALDI Mass Spectrometry |
| title_full | Assessing Glycosphingolipid Profiles in Human Health and Disease Using Non-Imaging MALDI Mass Spectrometry |
| title_fullStr | Assessing Glycosphingolipid Profiles in Human Health and Disease Using Non-Imaging MALDI Mass Spectrometry |
| title_full_unstemmed | Assessing Glycosphingolipid Profiles in Human Health and Disease Using Non-Imaging MALDI Mass Spectrometry |
| title_short | Assessing Glycosphingolipid Profiles in Human Health and Disease Using Non-Imaging MALDI Mass Spectrometry |
| title_sort | assessing glycosphingolipid profiles in human health and disease using non imaging maldi mass spectrometry |
| topic | glycosphingolipids (GSLs) MALDI mass spectrometry (MS) healthy human tissues pathological human tissues cell cultures |
| url | https://www.mdpi.com/2076-3417/13/17/9922 |
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