Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9
The cryo-EM structure of the IscB–ωRNA–target DNA complex provide insights into the ωRNA-guided DNA cleavage mechanism by IscB and the evolution of the type II CRISPR-Cas9 effector complexes
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-34378-3 |
| _version_ | 1811230229500264448 |
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| author | Kazuki Kato Sae Okazaki Soumya Kannan Han Altae-Tran F. Esra Demircioglu Yukari Isayama Junichiro Ishikawa Masahiro Fukuda Rhiannon K. Macrae Tomohiro Nishizawa Kira S. Makarova Eugene V. Koonin Feng Zhang Hiroshi Nishimasu |
| author_facet | Kazuki Kato Sae Okazaki Soumya Kannan Han Altae-Tran F. Esra Demircioglu Yukari Isayama Junichiro Ishikawa Masahiro Fukuda Rhiannon K. Macrae Tomohiro Nishizawa Kira S. Makarova Eugene V. Koonin Feng Zhang Hiroshi Nishimasu |
| author_sort | Kazuki Kato |
| collection | DOAJ |
| description | The cryo-EM structure of the IscB–ωRNA–target DNA complex provide insights into the ωRNA-guided DNA cleavage mechanism by IscB and the evolution of the type II CRISPR-Cas9 effector complexes |
| first_indexed | 2024-04-12T10:27:37Z |
| format | Article |
| id | doaj.art-f0ba686eba53416aba3c9403c2e20cc5 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T10:27:37Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-f0ba686eba53416aba3c9403c2e20cc52022-12-22T03:36:57ZengNature PortfolioNature Communications2041-17232022-11-0113111010.1038/s41467-022-34378-3Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9Kazuki Kato0Sae Okazaki1Soumya Kannan2Han Altae-Tran3F. Esra Demircioglu4Yukari Isayama5Junichiro Ishikawa6Masahiro Fukuda7Rhiannon K. Macrae8Tomohiro Nishizawa9Kira S. Makarova10Eugene V. Koonin11Feng Zhang12Hiroshi Nishimasu13Structural Biology Division, Research Center for Advanced Science and Technology, The University of TokyoStructural Biology Division, Research Center for Advanced Science and Technology, The University of TokyoMcGovern Institute for Brain Research at MIT, Massachusetts Institute of TechnologyMcGovern Institute for Brain Research at MIT, Massachusetts Institute of TechnologyMcGovern Institute for Brain Research at MIT, Massachusetts Institute of TechnologyStructural Biology Division, Research Center for Advanced Science and Technology, The University of TokyoStructural Biology Division, Research Center for Advanced Science and Technology, The University of TokyoKomaba Institute for Science, The University of Tokyo, MeguroMcGovern Institute for Brain Research at MIT, Massachusetts Institute of TechnologyGraduate School of Medical Life Science, Yokohama City UniversityNational Center for Biotechnology Information, National Library of Medicine, National Institutes of HealthNational Center for Biotechnology Information, National Library of Medicine, National Institutes of HealthMcGovern Institute for Brain Research at MIT, Massachusetts Institute of TechnologyStructural Biology Division, Research Center for Advanced Science and Technology, The University of TokyoThe cryo-EM structure of the IscB–ωRNA–target DNA complex provide insights into the ωRNA-guided DNA cleavage mechanism by IscB and the evolution of the type II CRISPR-Cas9 effector complexeshttps://doi.org/10.1038/s41467-022-34378-3 |
| spellingShingle | Kazuki Kato Sae Okazaki Soumya Kannan Han Altae-Tran F. Esra Demircioglu Yukari Isayama Junichiro Ishikawa Masahiro Fukuda Rhiannon K. Macrae Tomohiro Nishizawa Kira S. Makarova Eugene V. Koonin Feng Zhang Hiroshi Nishimasu Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9 Nature Communications |
| title | Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9 |
| title_full | Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9 |
| title_fullStr | Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9 |
| title_full_unstemmed | Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9 |
| title_short | Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9 |
| title_sort | structure of the iscb ωrna ribonucleoprotein complex the likely ancestor of crispr cas9 |
| url | https://doi.org/10.1038/s41467-022-34378-3 |
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