DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS
Present study has shown that a total cytokine pool produced by human blood cells in presence of proteins from the γ-globulin fraction, or their complexes with copper and zinc, as well as with pure copper and zinc cations, contains up to 148.0±14.9 pg interleukin-1β (IL-1β) per mL. The γ-globulins mo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/437 |
| _version_ | 1797198126652588032 |
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| author | S. B. Cheknev I. E. Efremova L. S. Piskovskaya E. N. Yushkovets A. A. Babajanz |
| author_facet | S. B. Cheknev I. E. Efremova L. S. Piskovskaya E. N. Yushkovets A. A. Babajanz |
| author_sort | S. B. Cheknev |
| collection | DOAJ |
| description | Present study has shown that a total cytokine pool produced by human blood cells in presence of proteins from the γ-globulin fraction, or their complexes with copper and zinc, as well as with pure copper and zinc cations, contains up to 148.0±14.9 pg interleukin-1β (IL-1β) per mL. The γ-globulins modified by copper or zinc binding induce, resp., 2.0 and 1.4-fold less IL-1β than intact proteins (significant by p = 0.1). Meanwhile, the copper-γ-globulin proved to be 2.7-fold more active (р < 0.01) IL-1β inducer, as a zinc-protein complex. Pure zinc ions did not induce IL-1β production, whereas induction rates with pure copper ions are 1.6-fold higher (p < 0.05) than with γ-globulin, and 1.45-fold more efficient that PHA stimulation (p < 0.1). A potential participation of copper and zinc cations bound to antibodies from the microenvironment and, thus, transforming Fc regions of antibody molecules, is discussed with regard of regulation of IL-1β production by human blood cells induced by activation of their Fc receptors. |
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| format | Article |
| id | doaj.art-f0bb304fb0584dddb2a14ede7e62e3d0 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:54:54Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-f0bb304fb0584dddb2a14ede7e62e3d02024-04-22T13:07:36ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0112649750210.15789/1563-0625-2010-6-497-502434DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALSS. B. Cheknev0I. E. Efremova1L. S. Piskovskaya2E. N. Yushkovets3A. A. Babajanz4Лаборатория межклеточных взаимодействий НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи Минздравсоцразвития РоссииЛаборатория межклеточных взаимодействий НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи Минздравсоцразвития РоссииЛаборатория межклеточных взаимодействий НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи Минздравсоцразвития РоссииЛаборатория межклеточных взаимодействий НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи Минздравсоцразвития РоссииЛаборатория межклеточных взаимодействий НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи Минздравсоцразвития РоссииPresent study has shown that a total cytokine pool produced by human blood cells in presence of proteins from the γ-globulin fraction, or their complexes with copper and zinc, as well as with pure copper and zinc cations, contains up to 148.0±14.9 pg interleukin-1β (IL-1β) per mL. The γ-globulins modified by copper or zinc binding induce, resp., 2.0 and 1.4-fold less IL-1β than intact proteins (significant by p = 0.1). Meanwhile, the copper-γ-globulin proved to be 2.7-fold more active (р < 0.01) IL-1β inducer, as a zinc-protein complex. Pure zinc ions did not induce IL-1β production, whereas induction rates with pure copper ions are 1.6-fold higher (p < 0.05) than with γ-globulin, and 1.45-fold more efficient that PHA stimulation (p < 0.1). A potential participation of copper and zinc cations bound to antibodies from the microenvironment and, thus, transforming Fc regions of antibody molecules, is discussed with regard of regulation of IL-1β production by human blood cells induced by activation of their Fc receptors.https://www.mimmun.ru/mimmun/article/view/437γ-globulinmetal complexesil-1βinductionproduction |
| spellingShingle | S. B. Cheknev I. E. Efremova L. S. Piskovskaya E. N. Yushkovets A. A. Babajanz DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS Медицинская иммунология γ-globulin metal complexes il-1β induction production |
| title | DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS |
| title_full | DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS |
| title_fullStr | DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS |
| title_full_unstemmed | DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS |
| title_short | DETECTION OF IL-1β PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE γ-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS |
| title_sort | detection of il 1β produced by human blood cells in presence of proteins from the γ globulin fraction and their complexes with metals |
| topic | γ-globulin metal complexes il-1β induction production |
| url | https://www.mimmun.ru/mimmun/article/view/437 |
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