Genotyping of Salmonella Enterica Serovar Enteritidis Strains Isolated from Clinical Samples by Pulsed-Field Gel Electrophoresis (PFGE)
Background: The infections caused by Salmonella enterica serovar enteritidis (Salmonella enteritidis) have been increasing in developed and developing countries. The aim of this study was to investigate the genetic relationship between the strains of Salmonella enteritidis. Methods: In a descriptiv...
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| Format: | Article |
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Isfahan University of Medical Sciences
2013-08-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2572 |
| _version_ | 1797762560334757888 |
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| author | Zeinab Ahmadi Reza Ranjbar Meysam Sarshar |
| author_facet | Zeinab Ahmadi Reza Ranjbar Meysam Sarshar |
| author_sort | Zeinab Ahmadi |
| collection | DOAJ |
| description | Background: The infections caused by Salmonella enterica serovar enteritidis (Salmonella enteritidis) have been increasing in developed and developing countries. The aim of this study was to investigate the genetic relationship between the strains of Salmonella enteritidis.
Methods: In a descriptive study from 2008 to 2010, clinical Salmonella enteritidis strains were collected from the patients referred to different hospitals in Tehran. The strains identified by biochemical and serological methods. Genetic relationships between the isolates were investigated using pulsed field gel electrophoresis (PFGE) method.
Findings: Of 40 Salmonella enteritidis isolated strains, two PFGE profiles were obtained which among them, 32 (80%) and 8 (20%) of isolates belonged to cluster A and cluster B, respectively. Based on PFGE patterns, 28 (70%) strains exhibited nine bands and were belonged to cluster A.
Conclusion: The result of this study indicated that isolated Salmonella enteritidis strains belonged to the closed clones. |
| first_indexed | 2024-03-12T19:30:07Z |
| format | Article |
| id | doaj.art-f0c0b3c4bc144c8e9e1843d8e9766317 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T19:30:07Z |
| publishDate | 2013-08-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-f0c0b3c4bc144c8e9e1843d8e97663172023-08-02T04:40:16ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2013-08-01312408198291355Genotyping of Salmonella Enterica Serovar Enteritidis Strains Isolated from Clinical Samples by Pulsed-Field Gel Electrophoresis (PFGE)Zeinab Ahmadi0Reza Ranjbar1Meysam Sarshar2Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranAssociate Professor, Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranMolecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranBackground: The infections caused by Salmonella enterica serovar enteritidis (Salmonella enteritidis) have been increasing in developed and developing countries. The aim of this study was to investigate the genetic relationship between the strains of Salmonella enteritidis. Methods: In a descriptive study from 2008 to 2010, clinical Salmonella enteritidis strains were collected from the patients referred to different hospitals in Tehran. The strains identified by biochemical and serological methods. Genetic relationships between the isolates were investigated using pulsed field gel electrophoresis (PFGE) method. Findings: Of 40 Salmonella enteritidis isolated strains, two PFGE profiles were obtained which among them, 32 (80%) and 8 (20%) of isolates belonged to cluster A and cluster B, respectively. Based on PFGE patterns, 28 (70%) strains exhibited nine bands and were belonged to cluster A. Conclusion: The result of this study indicated that isolated Salmonella enteritidis strains belonged to the closed clones.http://jims.mui.ac.ir/index.php/jims/article/view/2572Salmonella enteritidisGenotypingPulsed-field gel electrophoresis |
| spellingShingle | Zeinab Ahmadi Reza Ranjbar Meysam Sarshar Genotyping of Salmonella Enterica Serovar Enteritidis Strains Isolated from Clinical Samples by Pulsed-Field Gel Electrophoresis (PFGE) مجله دانشکده پزشکی اصفهان Salmonella enteritidis Genotyping Pulsed-field gel electrophoresis |
| title | Genotyping of Salmonella Enterica Serovar Enteritidis Strains Isolated from Clinical Samples by Pulsed-Field Gel Electrophoresis (PFGE) |
| title_full | Genotyping of Salmonella Enterica Serovar Enteritidis Strains Isolated from Clinical Samples by Pulsed-Field Gel Electrophoresis (PFGE) |
| title_fullStr | Genotyping of Salmonella Enterica Serovar Enteritidis Strains Isolated from Clinical Samples by Pulsed-Field Gel Electrophoresis (PFGE) |
| title_full_unstemmed | Genotyping of Salmonella Enterica Serovar Enteritidis Strains Isolated from Clinical Samples by Pulsed-Field Gel Electrophoresis (PFGE) |
| title_short | Genotyping of Salmonella Enterica Serovar Enteritidis Strains Isolated from Clinical Samples by Pulsed-Field Gel Electrophoresis (PFGE) |
| title_sort | genotyping of salmonella enterica serovar enteritidis strains isolated from clinical samples by pulsed field gel electrophoresis pfge |
| topic | Salmonella enteritidis Genotyping Pulsed-field gel electrophoresis |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2572 |
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