Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters
This study investigated the effects of conditioned medium (CM) from canine amniotic membrane-derived MSCs (cAMSCs) on dog sperm cryopreservation. For this purpose, flow cytometry analysis was performed to characterize cAMSCs. The CM prepared from cAMSCs was subjected to proteomic analysis for the id...
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MDPI AG
2020-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/10/1899 |
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| author | Feriel Yasmine Mahiddine Jin Wook Kim Ahmad Yar Qamar Jeong Chan Ra Soo Hyun Kim Eun Joong Jung Min Jung Kim |
| author_facet | Feriel Yasmine Mahiddine Jin Wook Kim Ahmad Yar Qamar Jeong Chan Ra Soo Hyun Kim Eun Joong Jung Min Jung Kim |
| author_sort | Feriel Yasmine Mahiddine |
| collection | DOAJ |
| description | This study investigated the effects of conditioned medium (CM) from canine amniotic membrane-derived MSCs (cAMSCs) on dog sperm cryopreservation. For this purpose, flow cytometry analysis was performed to characterize cAMSCs. The CM prepared from cAMSCs was subjected to proteomic analysis for the identification of proteins present in the medium. Sperm samples were treated with freezing medium supplemented with 0%, 5%, 10%, and 15% of the CM, and kinetic parameters were evaluated after 4–6 h of chilling at 4 °C to select the best concentration before proceeding to cryopreservation. Quality-related parameters of frozen–thawed sperm were investigated, including motility; kinetic parameters; viability; integrity of the plasma membrane, chromatin, and acrosome; and mitochondrial activity. The results showed that 10% of the CM significantly enhanced motility, viability, mitochondrial activity, and membrane integrity (<i>p</i> < 0.05); however, the analysis of chromatin and acrosome integrity showed no significant differences between the treatment and control groups. Therefore, we concluded that the addition of 10% CM derived from cAMSC in the freezing medium protected dog sperm during the cryopreservation process. |
| first_indexed | 2024-03-10T15:34:03Z |
| format | Article |
| id | doaj.art-f0c0e58c0fa4470eb3269d3763737820 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T15:34:03Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-f0c0e58c0fa4470eb3269d37637378202023-11-20T17:25:27ZengMDPI AGAnimals2076-26152020-10-011010189910.3390/ani10101899Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related ParametersFeriel Yasmine Mahiddine0Jin Wook Kim1Ahmad Yar Qamar2Jeong Chan Ra3Soo Hyun Kim4Eun Joong Jung5Min Jung Kim6Department of Theriogenology and Biotechnologies, College of Veterinary Medicine, Seoul National University, Seoul 08826, KoreaDepartment of Theriogenology and Biotechnologies, College of Veterinary Medicine, Seoul National University, Seoul 08826, KoreaLaboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, KoreaCell Physiology Research Center, Naturecell Co., Ltd., Seoul 07238, KoreaCell Physiology Research Center, Naturecell Co., Ltd., Seoul 07238, KoreaCell Physiology Research Center, Naturecell Co., Ltd., Seoul 07238, KoreaDepartment of Theriogenology and Biotechnologies, College of Veterinary Medicine, Seoul National University, Seoul 08826, KoreaThis study investigated the effects of conditioned medium (CM) from canine amniotic membrane-derived MSCs (cAMSCs) on dog sperm cryopreservation. For this purpose, flow cytometry analysis was performed to characterize cAMSCs. The CM prepared from cAMSCs was subjected to proteomic analysis for the identification of proteins present in the medium. Sperm samples were treated with freezing medium supplemented with 0%, 5%, 10%, and 15% of the CM, and kinetic parameters were evaluated after 4–6 h of chilling at 4 °C to select the best concentration before proceeding to cryopreservation. Quality-related parameters of frozen–thawed sperm were investigated, including motility; kinetic parameters; viability; integrity of the plasma membrane, chromatin, and acrosome; and mitochondrial activity. The results showed that 10% of the CM significantly enhanced motility, viability, mitochondrial activity, and membrane integrity (<i>p</i> < 0.05); however, the analysis of chromatin and acrosome integrity showed no significant differences between the treatment and control groups. Therefore, we concluded that the addition of 10% CM derived from cAMSC in the freezing medium protected dog sperm during the cryopreservation process.https://www.mdpi.com/2076-2615/10/10/1899sperm cryopreservationcanineamniotic membranestem cellsconditioned mediumproteomics |
| spellingShingle | Feriel Yasmine Mahiddine Jin Wook Kim Ahmad Yar Qamar Jeong Chan Ra Soo Hyun Kim Eun Joong Jung Min Jung Kim Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters Animals sperm cryopreservation canine amniotic membrane stem cells conditioned medium proteomics |
| title | Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters |
| title_full | Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters |
| title_fullStr | Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters |
| title_full_unstemmed | Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters |
| title_short | Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters |
| title_sort | conditioned medium from canine amniotic membrane derived mesenchymal stem cells improved dog sperm post thaw quality related parameters |
| topic | sperm cryopreservation canine amniotic membrane stem cells conditioned medium proteomics |
| url | https://www.mdpi.com/2076-2615/10/10/1899 |
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