Assessment of Erythrocyte Osmotic Fragility and Its Determinants, and Comparison of Hematological Indices Among Type 2 Diabetes Mellitus Patients on Follow-Up at Jimma Medical Center, Southwest Ethiopia
Zenebe Tujara,1 Wondu Reta,2 Eyasu Tadesse,2 Ifa Dereje,1 Mekonnen Tesfa1 1Department of Medicine, College of Health sciences, Salale University, Fiche, Oromia, Ethiopia; 2Department of Biomedical Sciences, Institute of Health, Jimma University, Jimma, Oromia, EthiopiaCorrespondence: Zenebe Tujara,...
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Dove Medical Press
2024-01-01
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| Series: | Journal of Blood Medicine |
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| Online Access: | https://www.dovepress.com/assessment-of-erythrocyte-osmotic-fragility-and-its-determinants-and-c-peer-reviewed-fulltext-article-JBM |
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| author | Tujara Z Reta W Tadesse E Dereje I Tesfa M |
| author_facet | Tujara Z Reta W Tadesse E Dereje I Tesfa M |
| author_sort | Tujara Z |
| collection | DOAJ |
| description | Zenebe Tujara,1 Wondu Reta,2 Eyasu Tadesse,2 Ifa Dereje,1 Mekonnen Tesfa1 1Department of Medicine, College of Health sciences, Salale University, Fiche, Oromia, Ethiopia; 2Department of Biomedical Sciences, Institute of Health, Jimma University, Jimma, Oromia, EthiopiaCorrespondence: Zenebe Tujara, Tel +251-917796943, Email zenebetujara837@gmail.comBackground: Diabetes Mellitus (DM) is one of the most prevalent non-communicable diseases worldwide. Approximately 9.3% of the general population was estimated to have DM globally in 2019. Erythrocyte osmotic fragility (EOF) in hyperglycemic patients is expected to increase and determine the rate of erythrocyte hemolysis.Purpose: This study aimed to assess erythrocyte osmotic fragility (EOF) and its determinants and to compare hematological indices among T2DM patients on follow-up at the Jimma Medical Center (JMC), Jimma, Southwest Ethiopia.Methods: A facility-based cross-sectional study involving 124 participants (each 62) of T2DM patients and controls was conducted from October to November 2020 using a structured questionnaire. 5 mL of venous blood was drawn to assess OF, complete blood count, and blood glucose levels. EOF was investigated using a series hypotonic solution of NaCl. The supernatant of the centrifuged sample was transferred to cuvette test tubes, and the hemolysis stage was read on a spectrophotometer. The collected data were coded and entered into Epi-data Version 3.1. The analysis was performed using SPSS Version 23.Results: Compared with non-diabetic controls, patients with T2DM had significantly increased EOF. FBG > 126mg/dl (AOR=7.741, 95% CI: 1.562– 38.360), PPBG > 200 mg/dl (AOR=7.576, 95% CI: 1.519– 37.791), RDW (AOR=4.558, 95% CI: 1.136– 18.284) were significantly associated with abnormal EOF. A statistically significant increase in total white blood cells and absolute neutrophil counts (P < 0.001) were observed in T2DM patients. From RBC indices, red blood cell distribution width (RDW) and mean corpuscular volume (MCV) were significantly increased in T2DM patients (P < 0.001).Conclusion: This study suggests that EOF was greater in patients with T2DM than in non-diabetic controls and was determined by FBG, PPBG, and RDW. The study also demonstrated that hematological index alterations were higher in T2DM subjects than in non-diabetic controls.Keywords: diabetes, osmotic fragility, hematological indices, RBC |
| first_indexed | 2024-03-08T12:02:07Z |
| format | Article |
| id | doaj.art-f0c71d667bcc43b7ac90233f9925e8d3 |
| institution | Directory Open Access Journal |
| issn | 1179-2736 |
| language | English |
| last_indexed | 2024-03-08T12:02:07Z |
| publishDate | 2024-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Blood Medicine |
| spelling | doaj.art-f0c71d667bcc43b7ac90233f9925e8d32024-01-23T18:27:27ZengDove Medical PressJournal of Blood Medicine1179-27362024-01-01Volume 1591989877Assessment of Erythrocyte Osmotic Fragility and Its Determinants, and Comparison of Hematological Indices Among Type 2 Diabetes Mellitus Patients on Follow-Up at Jimma Medical Center, Southwest EthiopiaTujara ZReta WTadesse EDereje ITesfa MZenebe Tujara,1 Wondu Reta,2 Eyasu Tadesse,2 Ifa Dereje,1 Mekonnen Tesfa1 1Department of Medicine, College of Health sciences, Salale University, Fiche, Oromia, Ethiopia; 2Department of Biomedical Sciences, Institute of Health, Jimma University, Jimma, Oromia, EthiopiaCorrespondence: Zenebe Tujara, Tel +251-917796943, Email zenebetujara837@gmail.comBackground: Diabetes Mellitus (DM) is one of the most prevalent non-communicable diseases worldwide. Approximately 9.3% of the general population was estimated to have DM globally in 2019. Erythrocyte osmotic fragility (EOF) in hyperglycemic patients is expected to increase and determine the rate of erythrocyte hemolysis.Purpose: This study aimed to assess erythrocyte osmotic fragility (EOF) and its determinants and to compare hematological indices among T2DM patients on follow-up at the Jimma Medical Center (JMC), Jimma, Southwest Ethiopia.Methods: A facility-based cross-sectional study involving 124 participants (each 62) of T2DM patients and controls was conducted from October to November 2020 using a structured questionnaire. 5 mL of venous blood was drawn to assess OF, complete blood count, and blood glucose levels. EOF was investigated using a series hypotonic solution of NaCl. The supernatant of the centrifuged sample was transferred to cuvette test tubes, and the hemolysis stage was read on a spectrophotometer. The collected data were coded and entered into Epi-data Version 3.1. The analysis was performed using SPSS Version 23.Results: Compared with non-diabetic controls, patients with T2DM had significantly increased EOF. FBG > 126mg/dl (AOR=7.741, 95% CI: 1.562– 38.360), PPBG > 200 mg/dl (AOR=7.576, 95% CI: 1.519– 37.791), RDW (AOR=4.558, 95% CI: 1.136– 18.284) were significantly associated with abnormal EOF. A statistically significant increase in total white blood cells and absolute neutrophil counts (P < 0.001) were observed in T2DM patients. From RBC indices, red blood cell distribution width (RDW) and mean corpuscular volume (MCV) were significantly increased in T2DM patients (P < 0.001).Conclusion: This study suggests that EOF was greater in patients with T2DM than in non-diabetic controls and was determined by FBG, PPBG, and RDW. The study also demonstrated that hematological index alterations were higher in T2DM subjects than in non-diabetic controls.Keywords: diabetes, osmotic fragility, hematological indices, RBChttps://www.dovepress.com/assessment-of-erythrocyte-osmotic-fragility-and-its-determinants-and-c-peer-reviewed-fulltext-article-JBMdiabetesosmotic fragilityhematological indicesrbc |
| spellingShingle | Tujara Z Reta W Tadesse E Dereje I Tesfa M Assessment of Erythrocyte Osmotic Fragility and Its Determinants, and Comparison of Hematological Indices Among Type 2 Diabetes Mellitus Patients on Follow-Up at Jimma Medical Center, Southwest Ethiopia Journal of Blood Medicine diabetes osmotic fragility hematological indices rbc |
| title | Assessment of Erythrocyte Osmotic Fragility and Its Determinants, and Comparison of Hematological Indices Among Type 2 Diabetes Mellitus Patients on Follow-Up at Jimma Medical Center, Southwest Ethiopia |
| title_full | Assessment of Erythrocyte Osmotic Fragility and Its Determinants, and Comparison of Hematological Indices Among Type 2 Diabetes Mellitus Patients on Follow-Up at Jimma Medical Center, Southwest Ethiopia |
| title_fullStr | Assessment of Erythrocyte Osmotic Fragility and Its Determinants, and Comparison of Hematological Indices Among Type 2 Diabetes Mellitus Patients on Follow-Up at Jimma Medical Center, Southwest Ethiopia |
| title_full_unstemmed | Assessment of Erythrocyte Osmotic Fragility and Its Determinants, and Comparison of Hematological Indices Among Type 2 Diabetes Mellitus Patients on Follow-Up at Jimma Medical Center, Southwest Ethiopia |
| title_short | Assessment of Erythrocyte Osmotic Fragility and Its Determinants, and Comparison of Hematological Indices Among Type 2 Diabetes Mellitus Patients on Follow-Up at Jimma Medical Center, Southwest Ethiopia |
| title_sort | assessment of erythrocyte osmotic fragility and its determinants and comparison of hematological indices among type 2 diabetes mellitus patients on follow up at jimma medical center southwest ethiopia |
| topic | diabetes osmotic fragility hematological indices rbc |
| url | https://www.dovepress.com/assessment-of-erythrocyte-osmotic-fragility-and-its-determinants-and-c-peer-reviewed-fulltext-article-JBM |
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