The Sweetpotato Voltage-Gated K<sup>+</sup> Channel β Subunit, KIbB1, Positively Regulates Low-K<sup>+</sup> and High-Salinity Tolerance by Maintaining Ion Homeostasis
Voltage-gated K<sup>+</sup> channel β subunits act as a structural component of K<sub>in</sub> channels in different species. The β subunits are not essential to the channel activity but confer different properties through binding the T1 domain or the C-terminal of α subunits...
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MDPI AG
2022-06-01
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| Online Access: | https://www.mdpi.com/2073-4425/13/6/1100 |
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| author | Hong Zhu Xue Yang Qiyan Li Jiayu Guo Tao Ma Shuyan Liu Shunyu Lin Yuanyuan Zhou Chunmei Zhao Jingshan Wang Jiongming Sui |
| author_facet | Hong Zhu Xue Yang Qiyan Li Jiayu Guo Tao Ma Shuyan Liu Shunyu Lin Yuanyuan Zhou Chunmei Zhao Jingshan Wang Jiongming Sui |
| author_sort | Hong Zhu |
| collection | DOAJ |
| description | Voltage-gated K<sup>+</sup> channel β subunits act as a structural component of K<sub>in</sub> channels in different species. The β subunits are not essential to the channel activity but confer different properties through binding the T1 domain or the C-terminal of α subunits. Here, we studied the physiological function of a novel gene, <i>KIbB1</i>, encoding a voltage-gated K<sup>+</sup> channel β subunit in sweetpotato. The transcriptional level of this gene was significantly higher in the low-K<sup>+</sup>-tolerant line than that in the low-K<sup>+</sup>-sensitive line under K<sup>+</sup> deficiency conditions. In <i>Arabidopsis</i>, <i>KIbB1</i> positively regulated low-K<sup>+</sup> tolerance through regulating K<sup>+</sup> uptake and translocation. Under high-salinity stress, the growth conditions of transgenic lines were obviously better than wild typr (WT). Enzymatic and non-enzymatic reactive oxygen species (ROS) scavenging were activated in transgenic plants. Accordingly, the malondialdehyde (MDA) content and the accumulation of ROS such as H<sub>2</sub>O<sub>2</sub> and O<sup>2−</sup> were lower in transgenic lines under salt stress. It was also found that the overexpression of <i>KIbB1</i> enhanced K<sup>+</sup> uptake, but the translocation from root to shoot was not affected under salt stress. This demonstrates that <i>KIbB1</i> acted as a positive regulator in high-salinity stress resistance through regulating Na<sup>+</sup> and K<sup>+</sup> uptake to maintain K<sup>+</sup>/Na<sup>+</sup> homeostasis. These results collectively suggest that the mechanisms of <i>KIbB1</i> in regulating K<sup>+</sup> were somewhat different between low-K<sup>+</sup> and high-salinity conditions. |
| first_indexed | 2024-03-09T23:43:23Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:43:23Z |
| publishDate | 2022-06-01 |
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| spelling | doaj.art-f0ca78bc8346472fbaf8d6b535e671372023-11-23T16:49:17ZengMDPI AGGenes2073-44252022-06-01136110010.3390/genes13061100The Sweetpotato Voltage-Gated K<sup>+</sup> Channel β Subunit, KIbB1, Positively Regulates Low-K<sup>+</sup> and High-Salinity Tolerance by Maintaining Ion HomeostasisHong Zhu0Xue Yang1Qiyan Li2Jiayu Guo3Tao Ma4Shuyan Liu5Shunyu Lin6Yuanyuan Zhou7Chunmei Zhao8Jingshan Wang9Jiongming Sui10College of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in Huang-Huai-Hai Region, Ministry of Agriculture and Rural Affairs, Jinan 250100, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaVoltage-gated K<sup>+</sup> channel β subunits act as a structural component of K<sub>in</sub> channels in different species. The β subunits are not essential to the channel activity but confer different properties through binding the T1 domain or the C-terminal of α subunits. Here, we studied the physiological function of a novel gene, <i>KIbB1</i>, encoding a voltage-gated K<sup>+</sup> channel β subunit in sweetpotato. The transcriptional level of this gene was significantly higher in the low-K<sup>+</sup>-tolerant line than that in the low-K<sup>+</sup>-sensitive line under K<sup>+</sup> deficiency conditions. In <i>Arabidopsis</i>, <i>KIbB1</i> positively regulated low-K<sup>+</sup> tolerance through regulating K<sup>+</sup> uptake and translocation. Under high-salinity stress, the growth conditions of transgenic lines were obviously better than wild typr (WT). Enzymatic and non-enzymatic reactive oxygen species (ROS) scavenging were activated in transgenic plants. Accordingly, the malondialdehyde (MDA) content and the accumulation of ROS such as H<sub>2</sub>O<sub>2</sub> and O<sup>2−</sup> were lower in transgenic lines under salt stress. It was also found that the overexpression of <i>KIbB1</i> enhanced K<sup>+</sup> uptake, but the translocation from root to shoot was not affected under salt stress. This demonstrates that <i>KIbB1</i> acted as a positive regulator in high-salinity stress resistance through regulating Na<sup>+</sup> and K<sup>+</sup> uptake to maintain K<sup>+</sup>/Na<sup>+</sup> homeostasis. These results collectively suggest that the mechanisms of <i>KIbB1</i> in regulating K<sup>+</sup> were somewhat different between low-K<sup>+</sup> and high-salinity conditions.https://www.mdpi.com/2073-4425/13/6/1100sweetpotatovoltage-gated K<sup>+</sup> channel β subunitKIbB1low K<sup>+</sup>high salinityion homeostasis |
| spellingShingle | Hong Zhu Xue Yang Qiyan Li Jiayu Guo Tao Ma Shuyan Liu Shunyu Lin Yuanyuan Zhou Chunmei Zhao Jingshan Wang Jiongming Sui The Sweetpotato Voltage-Gated K<sup>+</sup> Channel β Subunit, KIbB1, Positively Regulates Low-K<sup>+</sup> and High-Salinity Tolerance by Maintaining Ion Homeostasis Genes sweetpotato voltage-gated K<sup>+</sup> channel β subunit KIbB1 low K<sup>+</sup> high salinity ion homeostasis |
| title | The Sweetpotato Voltage-Gated K<sup>+</sup> Channel β Subunit, KIbB1, Positively Regulates Low-K<sup>+</sup> and High-Salinity Tolerance by Maintaining Ion Homeostasis |
| title_full | The Sweetpotato Voltage-Gated K<sup>+</sup> Channel β Subunit, KIbB1, Positively Regulates Low-K<sup>+</sup> and High-Salinity Tolerance by Maintaining Ion Homeostasis |
| title_fullStr | The Sweetpotato Voltage-Gated K<sup>+</sup> Channel β Subunit, KIbB1, Positively Regulates Low-K<sup>+</sup> and High-Salinity Tolerance by Maintaining Ion Homeostasis |
| title_full_unstemmed | The Sweetpotato Voltage-Gated K<sup>+</sup> Channel β Subunit, KIbB1, Positively Regulates Low-K<sup>+</sup> and High-Salinity Tolerance by Maintaining Ion Homeostasis |
| title_short | The Sweetpotato Voltage-Gated K<sup>+</sup> Channel β Subunit, KIbB1, Positively Regulates Low-K<sup>+</sup> and High-Salinity Tolerance by Maintaining Ion Homeostasis |
| title_sort | sweetpotato voltage gated k sup sup channel β subunit kibb1 positively regulates low k sup sup and high salinity tolerance by maintaining ion homeostasis |
| topic | sweetpotato voltage-gated K<sup>+</sup> channel β subunit KIbB1 low K<sup>+</sup> high salinity ion homeostasis |
| url | https://www.mdpi.com/2073-4425/13/6/1100 |
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