CORO1C, a novel PAK4 binding protein, recruits<?A3B2 ACK?>phospho-PAK4 at serine 99 to the leading edge and promotes the migration of gastric cancer cells
Gastric cancer is one of the malignant tumors in the world. PAK4 plays an important role in the occurrence and development of gastric cancer, especially in the process of invasion and metastasis. Here we discover that CORO1C, a member of coronin family that regulates microfilament and lamellipodia f...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
China Science Publishing & Media Ltd.
2022-05-01
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| Series: | Acta Biochimica et Biophysica Sinica |
| Subjects: | |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2022044 |
| _version_ | 1797635459688431616 |
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| author | Li Xiaodong Chen Min Yuan Ying Li Jiabin Li Feng |
| author_facet | Li Xiaodong Chen Min Yuan Ying Li Jiabin Li Feng |
| author_sort | Li Xiaodong |
| collection | DOAJ |
| description | Gastric cancer is one of the malignant tumors in the world. PAK4 plays an important role in the occurrence and development of gastric cancer, especially in the process of invasion and metastasis. Here we discover that CORO1C, a member of coronin family that regulates microfilament and lamellipodia formation, recruits cytoplasmic PAK4 to the leading edge of gastric cancer cells by C-terminal extension (CE) domain of CORO1C <sc>(353–457 aa).</sc> The localization of PAK4 on the leading edge of the cell depends on two necessary conditions: the phosphorylation of PAK4 on serine 99 and the binding to the CE domain of CORO1C. Unphosphorylated PAK4 on serine 99 is closely associated with microtubules by PAK4/GEF-H1/Tctex-1 complex. Once phosphorylated, PAK4 is released from microtubule, and then is recruited by CORO1C to the leading edge and regulates the CORO1C/RCC2 (regulator of chromosome condensation 2) complex, leading to the migration of gastric cancer cells. Our results reveal a new mechanism by which PAK4 regulates the migration potential of gastric cancer cells through microtubule-microfilament cross talk. |
| first_indexed | 2024-03-11T12:21:17Z |
| format | Article |
| id | doaj.art-f0cbf1e231044da8912f28d4ced23eec |
| institution | Directory Open Access Journal |
| issn | 1672-9145 |
| language | English |
| last_indexed | 2024-03-11T12:21:17Z |
| publishDate | 2022-05-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj.art-f0cbf1e231044da8912f28d4ced23eec2023-11-07T00:58:04ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452022-05-015467368510.3724/abbs.202204420d259ccCORO1C, a novel PAK4 binding protein, recruits<?A3B2 ACK?>phospho-PAK4 at serine 99 to the leading edge and promotes the migration of gastric cancer cellsLi Xiaodong0Chen Min1Yuan Ying2Li Jiabin3Li Feng4[][][][][]Gastric cancer is one of the malignant tumors in the world. PAK4 plays an important role in the occurrence and development of gastric cancer, especially in the process of invasion and metastasis. Here we discover that CORO1C, a member of coronin family that regulates microfilament and lamellipodia formation, recruits cytoplasmic PAK4 to the leading edge of gastric cancer cells by C-terminal extension (CE) domain of CORO1C <sc>(353–457 aa).</sc> The localization of PAK4 on the leading edge of the cell depends on two necessary conditions: the phosphorylation of PAK4 on serine 99 and the binding to the CE domain of CORO1C. Unphosphorylated PAK4 on serine 99 is closely associated with microtubules by PAK4/GEF-H1/Tctex-1 complex. Once phosphorylated, PAK4 is released from microtubule, and then is recruited by CORO1C to the leading edge and regulates the CORO1C/RCC2 (regulator of chromosome condensation 2) complex, leading to the migration of gastric cancer cells. Our results reveal a new mechanism by which PAK4 regulates the migration potential of gastric cancer cells through microtubule-microfilament cross talk.https://www.sciengine.com/doi/10.3724/abbs.2022044PAK4CORO1CRCC2microtubulemigrationgastric cancer cell |
| spellingShingle | Li Xiaodong Chen Min Yuan Ying Li Jiabin Li Feng CORO1C, a novel PAK4 binding protein, recruits<?A3B2 ACK?>phospho-PAK4 at serine 99 to the leading edge and promotes the migration of gastric cancer cells Acta Biochimica et Biophysica Sinica PAK4 CORO1C RCC2 microtubule migration gastric cancer cell |
| title | CORO1C, a novel PAK4 binding protein, recruits<?A3B2 ACK?>phospho-PAK4 at serine 99 to the leading edge and promotes the migration of gastric cancer cells |
| title_full | CORO1C, a novel PAK4 binding protein, recruits<?A3B2 ACK?>phospho-PAK4 at serine 99 to the leading edge and promotes the migration of gastric cancer cells |
| title_fullStr | CORO1C, a novel PAK4 binding protein, recruits<?A3B2 ACK?>phospho-PAK4 at serine 99 to the leading edge and promotes the migration of gastric cancer cells |
| title_full_unstemmed | CORO1C, a novel PAK4 binding protein, recruits<?A3B2 ACK?>phospho-PAK4 at serine 99 to the leading edge and promotes the migration of gastric cancer cells |
| title_short | CORO1C, a novel PAK4 binding protein, recruits<?A3B2 ACK?>phospho-PAK4 at serine 99 to the leading edge and promotes the migration of gastric cancer cells |
| title_sort | coro1c a novel pak4 binding protein recruits a3b2 ack phospho pak4 at serine 99 to the leading edge and promotes the migration of gastric cancer cells |
| topic | PAK4 CORO1C RCC2 microtubule migration gastric cancer cell |
| url | https://www.sciengine.com/doi/10.3724/abbs.2022044 |
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