Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts
Abstract Vascular graft surgeries are often conducted in trauma cases, which has increased the demand for scaffolds with good biocompatibility profiles. Biodegradable scaffolds resembling the extracellular matrix (ECM) of blood vessels are promising vascular graft materials. In the present study, po...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-03-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-09040-z |
| _version_ | 1819025349207392256 |
|---|---|
| author | Isabella C. P. Rodrigues Éder S. N. Lopes Karina D. Pereira Stephany C. Huber André Luiz Jardini Joyce M. Annichino-Bizzacchi Augusto D. Luchessi Laís P. Gabriel |
| author_facet | Isabella C. P. Rodrigues Éder S. N. Lopes Karina D. Pereira Stephany C. Huber André Luiz Jardini Joyce M. Annichino-Bizzacchi Augusto D. Luchessi Laís P. Gabriel |
| author_sort | Isabella C. P. Rodrigues |
| collection | DOAJ |
| description | Abstract Vascular graft surgeries are often conducted in trauma cases, which has increased the demand for scaffolds with good biocompatibility profiles. Biodegradable scaffolds resembling the extracellular matrix (ECM) of blood vessels are promising vascular graft materials. In the present study, polyurethane (PU) was blended with ECM proteins collagen and elastin (Col-El) and gelatin (Gel) to produce fibrous scaffolds by using the rotary jet spinning (RJS) technique, and their effects on in vitro properties were evaluated. Morphological and structural characterization of the scaffolds was performed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Micrometric fibers with nanometric rugosity were obtained. Col-El and Gel reduced the mechanical strength and increased the hydrophilicity and degradation rates of PU. No platelet adhesion or activation was observed. The addition of proteins to the PU blend increased the viability, adhesion, and proliferation of human umbilical vein endothelial cells (HUVECs). Therefore, PU-Col-El and PU-Gel scaffolds are promising biomaterials for vascular graft applications. |
| first_indexed | 2024-12-21T05:09:16Z |
| format | Article |
| id | doaj.art-f0cc655a06f14375937728e03db52185 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T05:09:16Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f0cc655a06f14375937728e03db521852022-12-21T19:15:07ZengNature PortfolioScientific Reports2045-23222022-03-0112111210.1038/s41598-022-09040-zExtracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular graftsIsabella C. P. Rodrigues0Éder S. N. Lopes1Karina D. Pereira2Stephany C. Huber3André Luiz Jardini4Joyce M. Annichino-Bizzacchi5Augusto D. Luchessi6Laís P. Gabriel7School of Applied Sciences, University of CampinasSchool of Mechanical Engineering, University of CampinasSchool of Applied Sciences, University of CampinasHematology and Hemotherapy Center, University of CampinasSchool of Chemical Engineering, University of CampinasHematology and Hemotherapy Center, University of CampinasSchool of Applied Sciences, University of CampinasSchool of Applied Sciences, University of CampinasAbstract Vascular graft surgeries are often conducted in trauma cases, which has increased the demand for scaffolds with good biocompatibility profiles. Biodegradable scaffolds resembling the extracellular matrix (ECM) of blood vessels are promising vascular graft materials. In the present study, polyurethane (PU) was blended with ECM proteins collagen and elastin (Col-El) and gelatin (Gel) to produce fibrous scaffolds by using the rotary jet spinning (RJS) technique, and their effects on in vitro properties were evaluated. Morphological and structural characterization of the scaffolds was performed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Micrometric fibers with nanometric rugosity were obtained. Col-El and Gel reduced the mechanical strength and increased the hydrophilicity and degradation rates of PU. No platelet adhesion or activation was observed. The addition of proteins to the PU blend increased the viability, adhesion, and proliferation of human umbilical vein endothelial cells (HUVECs). Therefore, PU-Col-El and PU-Gel scaffolds are promising biomaterials for vascular graft applications.https://doi.org/10.1038/s41598-022-09040-z |
| spellingShingle | Isabella C. P. Rodrigues Éder S. N. Lopes Karina D. Pereira Stephany C. Huber André Luiz Jardini Joyce M. Annichino-Bizzacchi Augusto D. Luchessi Laís P. Gabriel Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts Scientific Reports |
| title | Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts |
| title_full | Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts |
| title_fullStr | Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts |
| title_full_unstemmed | Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts |
| title_short | Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts |
| title_sort | extracellular matrix derived and low cost proteins to improve polyurethane based scaffolds for vascular grafts |
| url | https://doi.org/10.1038/s41598-022-09040-z |
| work_keys_str_mv | AT isabellacprodrigues extracellularmatrixderivedandlowcostproteinstoimprovepolyurethanebasedscaffoldsforvasculargrafts AT edersnlopes extracellularmatrixderivedandlowcostproteinstoimprovepolyurethanebasedscaffoldsforvasculargrafts AT karinadpereira extracellularmatrixderivedandlowcostproteinstoimprovepolyurethanebasedscaffoldsforvasculargrafts AT stephanychuber extracellularmatrixderivedandlowcostproteinstoimprovepolyurethanebasedscaffoldsforvasculargrafts AT andreluizjardini extracellularmatrixderivedandlowcostproteinstoimprovepolyurethanebasedscaffoldsforvasculargrafts AT joycemannichinobizzacchi extracellularmatrixderivedandlowcostproteinstoimprovepolyurethanebasedscaffoldsforvasculargrafts AT augustodluchessi extracellularmatrixderivedandlowcostproteinstoimprovepolyurethanebasedscaffoldsforvasculargrafts AT laispgabriel extracellularmatrixderivedandlowcostproteinstoimprovepolyurethanebasedscaffoldsforvasculargrafts |