Experimental model of nephropathy associated with diabetes mellitus in mice
ABSTRACT Purpose: Nontransmissible chronic diseases, such as diabetes mellitus (DM) and nephropathy, affect a significant portion of the population, often treated due to injuries that require healing and regeneration. To create an experimental model of associated comorbidities, for healing and rege...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2023-05-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502023000100211&lng=en&tlng=en |
| _version_ | 1827955927181950976 |
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| author | Pâmela Henrique Silva Patrícia Henrique Silva Adalberto Vieira Corazza Josivaldo Godoy da Silva Iandara Schettert Silva |
| author_facet | Pâmela Henrique Silva Patrícia Henrique Silva Adalberto Vieira Corazza Josivaldo Godoy da Silva Iandara Schettert Silva |
| author_sort | Pâmela Henrique Silva |
| collection | DOAJ |
| description | ABSTRACT Purpose: Nontransmissible chronic diseases, such as diabetes mellitus (DM) and nephropathy, affect a significant portion of the population, often treated due to injuries that require healing and regeneration. To create an experimental model of associated comorbidities, for healing and regeneration studies, protocols for induction of nephropathy by ischemia and reperfusion (I/R) and induction of DM by injection of streptozotocin (STZ) were associated. Methods: Sixty-four mice (Mus musculus), female, adult, Swiss strain, weighing approximately 20 g, were divided into four groups: G1: control (n = 24), G2: nephropathy group (N) (n = 7), G3, DM (n = 9), and G4: N+DM (n = 24). Arteriovenous stenosis (I/R) of the left kidney was performed as the first protocol. The animals received a hyperlipidemic diet for 7 days after the injection of STZ (150 mg/kg, via i.p.) and an aqueous glucose solution (10%) for 24 h. The animals in the G3 and G4 groups were observed for 14 days before receiving the diet and STZ. The evolution of nephropathy was observed using a urine test strip and the DM, through the analysis of blood glucose with a reagent strip on a digital monitor. Results: The ischemic induction protocols of nephropathy and DM with STZ, associated, were sustainable, low-cost, and without deaths. There were alterations compatible with initial renal alterations, in the first 14 days, such as increased urinary density, pH alteration, presence of glucose, proteins and leukocytes, when compared to the control group. DM was confirmed by the presence of hyperglycemia 7 days after induction and its evolution after 14 days. The animals in the G4 group showed constant weight loss when compared to the other groups. It was possible to observe morphological alterations in the kidneys submitted to I/R, regarding coloration, during surgery and after the end of the observation period, in the volume and size of the left kidney, when compared to the contralateral kidney. Conclusions: It was possible to induce nephropathy and DM associated in the same animal, in a simple way, confirmed with rapid tests, without losses, providing a basis for future studies. |
| first_indexed | 2024-04-09T14:54:49Z |
| format | Article |
| id | doaj.art-f0ce0369515740c080dc0fa84282d9a0 |
| institution | Directory Open Access Journal |
| issn | 1678-2674 |
| language | English |
| last_indexed | 2024-04-09T14:54:49Z |
| publishDate | 2023-05-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-f0ce0369515740c080dc0fa84282d9a02023-05-02T07:33:17ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742023-05-013810.1590/acb381123Experimental model of nephropathy associated with diabetes mellitus in micePâmela Henrique Silvahttps://orcid.org/0000-0003-1133-7752Patrícia Henrique Silvahttps://orcid.org/0000-0002-6905-5646Adalberto Vieira Corazzahttps://orcid.org/0000-0003-4888-1380Josivaldo Godoy da Silvahttps://orcid.org/0000-0003-0663-2087Iandara Schettert Silvahttps://orcid.org/0000-0001-9431-098XABSTRACT Purpose: Nontransmissible chronic diseases, such as diabetes mellitus (DM) and nephropathy, affect a significant portion of the population, often treated due to injuries that require healing and regeneration. To create an experimental model of associated comorbidities, for healing and regeneration studies, protocols for induction of nephropathy by ischemia and reperfusion (I/R) and induction of DM by injection of streptozotocin (STZ) were associated. Methods: Sixty-four mice (Mus musculus), female, adult, Swiss strain, weighing approximately 20 g, were divided into four groups: G1: control (n = 24), G2: nephropathy group (N) (n = 7), G3, DM (n = 9), and G4: N+DM (n = 24). Arteriovenous stenosis (I/R) of the left kidney was performed as the first protocol. The animals received a hyperlipidemic diet for 7 days after the injection of STZ (150 mg/kg, via i.p.) and an aqueous glucose solution (10%) for 24 h. The animals in the G3 and G4 groups were observed for 14 days before receiving the diet and STZ. The evolution of nephropathy was observed using a urine test strip and the DM, through the analysis of blood glucose with a reagent strip on a digital monitor. Results: The ischemic induction protocols of nephropathy and DM with STZ, associated, were sustainable, low-cost, and without deaths. There were alterations compatible with initial renal alterations, in the first 14 days, such as increased urinary density, pH alteration, presence of glucose, proteins and leukocytes, when compared to the control group. DM was confirmed by the presence of hyperglycemia 7 days after induction and its evolution after 14 days. The animals in the G4 group showed constant weight loss when compared to the other groups. It was possible to observe morphological alterations in the kidneys submitted to I/R, regarding coloration, during surgery and after the end of the observation period, in the volume and size of the left kidney, when compared to the contralateral kidney. Conclusions: It was possible to induce nephropathy and DM associated in the same animal, in a simple way, confirmed with rapid tests, without losses, providing a basis for future studies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502023000100211&lng=en&tlng=enKidneyStreptozotocinIschemiaReperfusion |
| spellingShingle | Pâmela Henrique Silva Patrícia Henrique Silva Adalberto Vieira Corazza Josivaldo Godoy da Silva Iandara Schettert Silva Experimental model of nephropathy associated with diabetes mellitus in mice Acta Cirúrgica Brasileira Kidney Streptozotocin Ischemia Reperfusion |
| title | Experimental model of nephropathy associated with diabetes mellitus in mice |
| title_full | Experimental model of nephropathy associated with diabetes mellitus in mice |
| title_fullStr | Experimental model of nephropathy associated with diabetes mellitus in mice |
| title_full_unstemmed | Experimental model of nephropathy associated with diabetes mellitus in mice |
| title_short | Experimental model of nephropathy associated with diabetes mellitus in mice |
| title_sort | experimental model of nephropathy associated with diabetes mellitus in mice |
| topic | Kidney Streptozotocin Ischemia Reperfusion |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502023000100211&lng=en&tlng=en |
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