Use Multiplex PCR For Detection of Some Virulence Genes of Klebsiella Isolated From Different Environmental loci
This study includes collected of 399 samples from different sources included clinical and non clinical samples. 293 clinical samples (urine, wounds, burns, stool, sputum). Non- clinical samples included 106 samples (water, soil) from November 2014 through February 2015. 51 samples were diagnosed as...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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University of Anbar
2015-04-01
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| Series: | مجلة جامعة الانبار للعلوم الصرفة |
| Subjects: | |
| Online Access: | https://juaps.uoanbar.edu.iq/article_124384_bb1fba75f7592bf2129dd022693cd274.pdf |
| _version_ | 1827396205283377152 |
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| author | Zena J. Mohammed Ali H. Abdulkareem Ahmed A. Sulaiman |
| author_facet | Zena J. Mohammed Ali H. Abdulkareem Ahmed A. Sulaiman |
| author_sort | Zena J. Mohammed |
| collection | DOAJ |
| description | This study includes collected of 399 samples from different sources included clinical and non clinical samples. 293 clinical samples (urine, wounds, burns, stool, sputum). Non- clinical samples included 106 samples (water, soil) from November 2014 through February 2015. 51 samples were diagnosed as K. pneumoniae using phenotypic, biochemical and cultural diagnosis features and definitely diagnosed with API20E and vaitek test. Results of antibiotic resistance against different antibiotics showed that K. pneumoniae isolated from clinical and non- clinical samples varied in their resistance to these antibiotics and all isolates were sensitive 100% to Imipenem. A number of specific primers were designed to detect bacteria by using distinct gene and also to detects resistance to antibiotics using multiplex PCR technology for the presence or absence of 8 genes(magA, rmpA, htrA, Uge-1, gent-2,blaCTX, terA-1,StrA-2) that encodes for main virulence factors to diagnose K. pneumoniae. The result showed that that 45 isolates had rmpA which might be used as detection marker, in the same reaction of multiplex PCR detection of 4 genes related with resistance to different antibiotic groups(StrA,terA,blaCTX,gent-2) were done all K,pneumoniae contained StrA gene. According to this study we can concluded that multiplex PCR can use to distinguish isolated bacteria from different sources more correctly and also to determine its antibiotic resistance that are widely used in short time and little effort without need to routine testing. |
| first_indexed | 2024-03-08T18:48:33Z |
| format | Article |
| id | doaj.art-f0cff6fb6cad48de97e18a01de6ce767 |
| institution | Directory Open Access Journal |
| issn | 1991-8941 2706-6703 |
| language | English |
| last_indexed | 2024-03-08T18:48:33Z |
| publishDate | 2015-04-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة جامعة الانبار للعلوم الصرفة |
| spelling | doaj.art-f0cff6fb6cad48de97e18a01de6ce7672023-12-28T21:57:52ZengUniversity of Anbarمجلة جامعة الانبار للعلوم الصرفة1991-89412706-67032015-04-0191606610.37652/juaps.2015.124384124384Use Multiplex PCR For Detection of Some Virulence Genes of Klebsiella Isolated From Different Environmental lociZena J. Mohammed0Ali H. Abdulkareem1Ahmed A. Sulaiman2University of Anbar/College of ScienceUniversity of Anbar/College of ScienceUniversity of Anbar/Desert Studies CenterThis study includes collected of 399 samples from different sources included clinical and non clinical samples. 293 clinical samples (urine, wounds, burns, stool, sputum). Non- clinical samples included 106 samples (water, soil) from November 2014 through February 2015. 51 samples were diagnosed as K. pneumoniae using phenotypic, biochemical and cultural diagnosis features and definitely diagnosed with API20E and vaitek test. Results of antibiotic resistance against different antibiotics showed that K. pneumoniae isolated from clinical and non- clinical samples varied in their resistance to these antibiotics and all isolates were sensitive 100% to Imipenem. A number of specific primers were designed to detect bacteria by using distinct gene and also to detects resistance to antibiotics using multiplex PCR technology for the presence or absence of 8 genes(magA, rmpA, htrA, Uge-1, gent-2,blaCTX, terA-1,StrA-2) that encodes for main virulence factors to diagnose K. pneumoniae. The result showed that that 45 isolates had rmpA which might be used as detection marker, in the same reaction of multiplex PCR detection of 4 genes related with resistance to different antibiotic groups(StrA,terA,blaCTX,gent-2) were done all K,pneumoniae contained StrA gene. According to this study we can concluded that multiplex PCR can use to distinguish isolated bacteria from different sources more correctly and also to determine its antibiotic resistance that are widely used in short time and little effort without need to routine testing.https://juaps.uoanbar.edu.iq/article_124384_bb1fba75f7592bf2129dd022693cd274.pdfmultiplex pcrantibiotic resistanceklebsiella pneumoniavirulence gene |
| spellingShingle | Zena J. Mohammed Ali H. Abdulkareem Ahmed A. Sulaiman Use Multiplex PCR For Detection of Some Virulence Genes of Klebsiella Isolated From Different Environmental loci مجلة جامعة الانبار للعلوم الصرفة multiplex pcr antibiotic resistance klebsiella pneumonia virulence gene |
| title | Use Multiplex PCR For Detection of Some Virulence Genes of Klebsiella Isolated From Different Environmental loci |
| title_full | Use Multiplex PCR For Detection of Some Virulence Genes of Klebsiella Isolated From Different Environmental loci |
| title_fullStr | Use Multiplex PCR For Detection of Some Virulence Genes of Klebsiella Isolated From Different Environmental loci |
| title_full_unstemmed | Use Multiplex PCR For Detection of Some Virulence Genes of Klebsiella Isolated From Different Environmental loci |
| title_short | Use Multiplex PCR For Detection of Some Virulence Genes of Klebsiella Isolated From Different Environmental loci |
| title_sort | use multiplex pcr for detection of some virulence genes of klebsiella isolated from different environmental loci |
| topic | multiplex pcr antibiotic resistance klebsiella pneumonia virulence gene |
| url | https://juaps.uoanbar.edu.iq/article_124384_bb1fba75f7592bf2129dd022693cd274.pdf |
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