Cucurbitacins: elucidation of their interactions with the cytoskeleton
Cucurbitacins, a class of toxic tetracyclic triterpenoids in Cucurbitaceae, modulate many molecular targets. Here we investigated the interactions of cucurbitacin B, E and I with cytoskeletal proteins such as microtubule and actin filaments. The effects of cucurbitacin B, E and I on microtubules and...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2017-05-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/3357.pdf |
| _version_ | 1797420593871585280 |
|---|---|
| author | Xiaojuan Wang Mine Tanaka Herbenya Silva Peixoto Michael Wink |
| author_facet | Xiaojuan Wang Mine Tanaka Herbenya Silva Peixoto Michael Wink |
| author_sort | Xiaojuan Wang |
| collection | DOAJ |
| description | Cucurbitacins, a class of toxic tetracyclic triterpenoids in Cucurbitaceae, modulate many molecular targets. Here we investigated the interactions of cucurbitacin B, E and I with cytoskeletal proteins such as microtubule and actin filaments. The effects of cucurbitacin B, E and I on microtubules and actin filaments were studied in living cells (Hela and U2OS) and in vitro using GFP markers, immunofluorescence staining and in vitro tubulin polymerization assay. Cucurbitacin B, E and I apparently affected microtubule structures in living cells and cucurbitacin E inhibited tubulin polymerization in vitro with IC50 value of 566.91 ± 113.5 µM. Cucurbitacin E did not affect the nucleation but inhibited the growth phase and steady state during microtubule assembly in vitro. In addition, cucurbitacin B, E and I all altered mitotic spindles and induced the cell cycle arrest at G2/M phase. Moreover, they all showed potent effects on actin cytoskeleton by affecting actin filaments through the depolymerization and aggregation. The interactions of cucubitacin B, E and I with microtubules and actin filaments present new insights into their modes of action. |
| first_indexed | 2024-03-09T07:04:41Z |
| format | Article |
| id | doaj.art-f0d557afe44e4750bc66ddf1cfd7de4d |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:04:41Z |
| publishDate | 2017-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-f0d557afe44e4750bc66ddf1cfd7de4d2023-12-03T09:45:59ZengPeerJ Inc.PeerJ2167-83592017-05-015e335710.7717/peerj.3357Cucurbitacins: elucidation of their interactions with the cytoskeletonXiaojuan Wang0Mine Tanaka1Herbenya Silva Peixoto2Michael Wink3Institute of Pharmacy and Molecular Biotechnology, Ruprecht-Karls-Universität Heidelberg, Heidelberg, GermanyInstitute of Pharmacy and Molecular Biotechnology, Ruprecht-Karls-Universität Heidelberg, Heidelberg, GermanyInstitute of Pharmacy and Molecular Biotechnology, Ruprecht-Karls-Universität Heidelberg, Heidelberg, GermanyInstitute of Pharmacy and Molecular Biotechnology, Ruprecht-Karls-Universität Heidelberg, Heidelberg, GermanyCucurbitacins, a class of toxic tetracyclic triterpenoids in Cucurbitaceae, modulate many molecular targets. Here we investigated the interactions of cucurbitacin B, E and I with cytoskeletal proteins such as microtubule and actin filaments. The effects of cucurbitacin B, E and I on microtubules and actin filaments were studied in living cells (Hela and U2OS) and in vitro using GFP markers, immunofluorescence staining and in vitro tubulin polymerization assay. Cucurbitacin B, E and I apparently affected microtubule structures in living cells and cucurbitacin E inhibited tubulin polymerization in vitro with IC50 value of 566.91 ± 113.5 µM. Cucurbitacin E did not affect the nucleation but inhibited the growth phase and steady state during microtubule assembly in vitro. In addition, cucurbitacin B, E and I all altered mitotic spindles and induced the cell cycle arrest at G2/M phase. Moreover, they all showed potent effects on actin cytoskeleton by affecting actin filaments through the depolymerization and aggregation. The interactions of cucubitacin B, E and I with microtubules and actin filaments present new insights into their modes of action.https://peerj.com/articles/3357.pdfCucurbitacinsCucurbitaceaeCytotoxicityActin filamentMicrotubule |
| spellingShingle | Xiaojuan Wang Mine Tanaka Herbenya Silva Peixoto Michael Wink Cucurbitacins: elucidation of their interactions with the cytoskeleton PeerJ Cucurbitacins Cucurbitaceae Cytotoxicity Actin filament Microtubule |
| title | Cucurbitacins: elucidation of their interactions with the cytoskeleton |
| title_full | Cucurbitacins: elucidation of their interactions with the cytoskeleton |
| title_fullStr | Cucurbitacins: elucidation of their interactions with the cytoskeleton |
| title_full_unstemmed | Cucurbitacins: elucidation of their interactions with the cytoskeleton |
| title_short | Cucurbitacins: elucidation of their interactions with the cytoskeleton |
| title_sort | cucurbitacins elucidation of their interactions with the cytoskeleton |
| topic | Cucurbitacins Cucurbitaceae Cytotoxicity Actin filament Microtubule |
| url | https://peerj.com/articles/3357.pdf |
| work_keys_str_mv | AT xiaojuanwang cucurbitacinselucidationoftheirinteractionswiththecytoskeleton AT minetanaka cucurbitacinselucidationoftheirinteractionswiththecytoskeleton AT herbenyasilvapeixoto cucurbitacinselucidationoftheirinteractionswiththecytoskeleton AT michaelwink cucurbitacinselucidationoftheirinteractionswiththecytoskeleton |