Supplementation of Carbohydrate to Enhance the α-amylase Production by Bacillus licheniformis ATCC 6346 in Presence of Seed Cakes
Aims: The effect of carbohydrate and amino acids on the production of a-amylase by Bacillus licheniformis ATCC 6346 was investigated. Methodology and results: To find out the influence of carbohydrate the total carbohydrate content of the medium containing different concentration (2-18 g/L) of defat...
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| Format: | Article |
| Language: | English |
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Malaysian Society for Microbiology
2012-01-01
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| Series: | Malaysian Journal of Microbiology |
| Subjects: | |
| Online Access: | http://web.usm.my/mjm/issues/vol8no4/Research%205.pdf |
| _version_ | 1818256070933479424 |
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| author | Vengadaramana, A. Vasanthy, A. Balakumar, S. |
| author_facet | Vengadaramana, A. Vasanthy, A. Balakumar, S. |
| author_sort | Vengadaramana, A. |
| collection | DOAJ |
| description | Aims: The effect of carbohydrate and amino acids on the production of a-amylase by Bacillus licheniformis ATCC 6346 was investigated. Methodology and results: To find out the influence of carbohydrate the total carbohydrate content of the medium containing different concentration (2-18 g/L) of defatted seed cake powder of sesamum and mustard containing medium was kept constant by the addition of soluble starch separately. The highest a-amylase activity obtained in the medium containing 18g/L mustard (59.11+b1.48 U/mL) and sesamum seed cake powder (55.23+b1.55 U/mL). The results indicated that under these conditions the carbohydrate content had no effect on the production of a-amylase. Effect of amino acids (0.2g/L of glycine, methionine, proline, lysine, leucine, threonine, serine, arginine, alanine, glutamic acid, tryptophan, glutamine, asparagine, histidine, valine, phenylalanine, isoleucine and mixture of amino acids) on the production of a-amylase in fermentation medium was investigated. Among the different amino acids supplemented, eight amino acids improved the a-amylase production but casaminoacids slightly inhibited the enzyme production. In presence of tryptophan highest enzyme activity was obtained than control. Conclusion, significance and impact of study: In these study amino acids especially tryptophan takes part in a particular role rather than carbohydrate in the production of a-amylase from B. licheniformis ATCC 6346. |
| first_indexed | 2024-12-12T17:21:55Z |
| format | Article |
| id | doaj.art-f0d6039e766443dcbe6614acde5e09ec |
| institution | Directory Open Access Journal |
| issn | 1823-8262 2231-7538 |
| language | English |
| last_indexed | 2024-12-12T17:21:55Z |
| publishDate | 2012-01-01 |
| publisher | Malaysian Society for Microbiology |
| record_format | Article |
| series | Malaysian Journal of Microbiology |
| spelling | doaj.art-f0d6039e766443dcbe6614acde5e09ec2022-12-22T00:17:38ZengMalaysian Society for MicrobiologyMalaysian Journal of Microbiology1823-82622231-75382012-01-0184242247Supplementation of Carbohydrate to Enhance the α-amylase Production by Bacillus licheniformis ATCC 6346 in Presence of Seed CakesVengadaramana, A.Vasanthy, A.Balakumar, S.Aims: The effect of carbohydrate and amino acids on the production of a-amylase by Bacillus licheniformis ATCC 6346 was investigated. Methodology and results: To find out the influence of carbohydrate the total carbohydrate content of the medium containing different concentration (2-18 g/L) of defatted seed cake powder of sesamum and mustard containing medium was kept constant by the addition of soluble starch separately. The highest a-amylase activity obtained in the medium containing 18g/L mustard (59.11+b1.48 U/mL) and sesamum seed cake powder (55.23+b1.55 U/mL). The results indicated that under these conditions the carbohydrate content had no effect on the production of a-amylase. Effect of amino acids (0.2g/L of glycine, methionine, proline, lysine, leucine, threonine, serine, arginine, alanine, glutamic acid, tryptophan, glutamine, asparagine, histidine, valine, phenylalanine, isoleucine and mixture of amino acids) on the production of a-amylase in fermentation medium was investigated. Among the different amino acids supplemented, eight amino acids improved the a-amylase production but casaminoacids slightly inhibited the enzyme production. In presence of tryptophan highest enzyme activity was obtained than control. Conclusion, significance and impact of study: In these study amino acids especially tryptophan takes part in a particular role rather than carbohydrate in the production of a-amylase from B. licheniformis ATCC 6346.http://web.usm.my/mjm/issues/vol8no4/Research%205.pdfAmino acidsa-amylaseCasaminoacidsBacillus licheniformis |
| spellingShingle | Vengadaramana, A. Vasanthy, A. Balakumar, S. Supplementation of Carbohydrate to Enhance the α-amylase Production by Bacillus licheniformis ATCC 6346 in Presence of Seed Cakes Malaysian Journal of Microbiology Amino acids a-amylase Casaminoacids Bacillus licheniformis |
| title | Supplementation of Carbohydrate to Enhance the α-amylase Production by Bacillus licheniformis ATCC 6346 in Presence of Seed Cakes |
| title_full | Supplementation of Carbohydrate to Enhance the α-amylase Production by Bacillus licheniformis ATCC 6346 in Presence of Seed Cakes |
| title_fullStr | Supplementation of Carbohydrate to Enhance the α-amylase Production by Bacillus licheniformis ATCC 6346 in Presence of Seed Cakes |
| title_full_unstemmed | Supplementation of Carbohydrate to Enhance the α-amylase Production by Bacillus licheniformis ATCC 6346 in Presence of Seed Cakes |
| title_short | Supplementation of Carbohydrate to Enhance the α-amylase Production by Bacillus licheniformis ATCC 6346 in Presence of Seed Cakes |
| title_sort | supplementation of carbohydrate to enhance the α amylase production by bacillus licheniformis atcc 6346 in presence of seed cakes |
| topic | Amino acids a-amylase Casaminoacids Bacillus licheniformis |
| url | http://web.usm.my/mjm/issues/vol8no4/Research%205.pdf |
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