Isolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation analysis
Summary: Neural stem cells (NSCs) from the subventricular zone (SVZ) of the mouse brain can be expanded in vitro and grown as neurospheres, which can be stored long-term in liquid nitrogen. Here, we present a protocol for isolation and culture of NSCs from the adult mouse SVZ. We describe how to gro...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722000338 |
| _version_ | 1818775400664268800 |
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| author | Daniel Z. Radecki Jayshree Samanta |
| author_facet | Daniel Z. Radecki Jayshree Samanta |
| author_sort | Daniel Z. Radecki |
| collection | DOAJ |
| description | Summary: Neural stem cells (NSCs) from the subventricular zone (SVZ) of the mouse brain can be expanded in vitro and grown as neurospheres, which can be stored long-term in liquid nitrogen. Here, we present a protocol for isolation and culture of NSCs from the adult mouse SVZ. We describe how to grow and expand primary NSCs to neurospheres, followed by differentiation and nucleofection/pharmacological treatments. Finally, we describe RNA extraction, EdU labeling of the cells, and immunofluorescent analysis to examine their proliferation.For complete details on the use and execution of this protocol, please refer to Radecki et al. (2020). |
| first_indexed | 2024-12-18T10:56:26Z |
| format | Article |
| id | doaj.art-f0d70f82a5274c8196b2440a2c9cbe45 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-18T10:56:26Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-f0d70f82a5274c8196b2440a2c9cbe452022-12-21T21:10:19ZengElsevierSTAR Protocols2666-16672022-03-0131101153Isolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation analysisDaniel Z. Radecki0Jayshree Samanta1Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA; Stem Cell and Regenerative Medicine Center, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USADepartment of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA; Stem Cell and Regenerative Medicine Center, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA; Corresponding authorSummary: Neural stem cells (NSCs) from the subventricular zone (SVZ) of the mouse brain can be expanded in vitro and grown as neurospheres, which can be stored long-term in liquid nitrogen. Here, we present a protocol for isolation and culture of NSCs from the adult mouse SVZ. We describe how to grow and expand primary NSCs to neurospheres, followed by differentiation and nucleofection/pharmacological treatments. Finally, we describe RNA extraction, EdU labeling of the cells, and immunofluorescent analysis to examine their proliferation.For complete details on the use and execution of this protocol, please refer to Radecki et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166722000338Cell BiologyCell cultureCell isolationNeuroscienceStem Cells |
| spellingShingle | Daniel Z. Radecki Jayshree Samanta Isolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation analysis STAR Protocols Cell Biology Cell culture Cell isolation Neuroscience Stem Cells |
| title | Isolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation analysis |
| title_full | Isolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation analysis |
| title_fullStr | Isolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation analysis |
| title_full_unstemmed | Isolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation analysis |
| title_short | Isolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation analysis |
| title_sort | isolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation analysis |
| topic | Cell Biology Cell culture Cell isolation Neuroscience Stem Cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722000338 |
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