CRABP2 Promotes the Proliferation of Dermal Papilla Cells via the Wnt/β-Catenin Pathway
In our previous study of Hu sheep hair follicles, we found that CRABP2 was highly expressed in DPCs, which suggested that CRABP2 may influence the number of DPCs. In the present study, we aimed to understand the effect of CRABP2 in Hu sheep dermal papilla cells (DPCs). First, we explored the influen...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/12/2033 |
| _version_ | 1797596502786310144 |
|---|---|
| author | Mingliang He Xiaoyang Lv Xiukai Cao Zehu Yuan Kai Quan Tesfaye Getachew Joram M. Mwacharo Aynalem Haile Yutao Li Shanhe Wang Wei Sun |
| author_facet | Mingliang He Xiaoyang Lv Xiukai Cao Zehu Yuan Kai Quan Tesfaye Getachew Joram M. Mwacharo Aynalem Haile Yutao Li Shanhe Wang Wei Sun |
| author_sort | Mingliang He |
| collection | DOAJ |
| description | In our previous study of Hu sheep hair follicles, we found that CRABP2 was highly expressed in DPCs, which suggested that CRABP2 may influence the number of DPCs. In the present study, we aimed to understand the effect of CRABP2 in Hu sheep dermal papilla cells (DPCs). First, we explored the influence of CRABP2 on the ability of Hu sheep DPCs’ proliferation. Based on the results obtained from some experiments, such as CCK-8, EDU, qPCR, and Western blot experiment, we found that the overexpression of CRABP2 facilitated the proliferation of DPCs compared to the negative control group. Then, we also detected the effect of CRABP2 on the Wnt/β-catenin pathway based on the important function of the Wnt/β-catenin pathway in hair follicles. The results showed that CRABP2 could activate the Wnt/β-catenin pathway in DPCs, and it rescues the proliferation of DPCs when the Wnt/β-catenin pathway was inhibited. In summary, our findings indicate that CRABP2 is a vital functional gene in the proliferation of Hu sheep DPCs. Our study will be of great use for revealing the roles of CRABP2 in the hair follicles of Hu sheep. |
| first_indexed | 2024-03-11T02:51:16Z |
| format | Article |
| id | doaj.art-f0da95b79a264bb0b75b01f582dbc2e3 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T02:51:16Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-f0da95b79a264bb0b75b01f582dbc2e32023-11-18T08:59:38ZengMDPI AGAnimals2076-26152023-06-011312203310.3390/ani13122033CRABP2 Promotes the Proliferation of Dermal Papilla Cells via the Wnt/β-Catenin PathwayMingliang He0Xiaoyang Lv1Xiukai Cao2Zehu Yuan3Kai Quan4Tesfaye Getachew5Joram M. Mwacharo6Aynalem Haile7Yutao Li8Shanhe Wang9Wei Sun10College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaInternational Centre for Agricultural Research in the Dry Areas, Addis Ababa 999047, EthiopiaInternational Centre for Agricultural Research in the Dry Areas, Addis Ababa 999047, EthiopiaInternational Centre for Agricultural Research in the Dry Areas, Addis Ababa 999047, EthiopiaCSIRO Agriculture and Food, 306 Carmody Rd., St. Lucia, QLD 4067, AustraliaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaIn our previous study of Hu sheep hair follicles, we found that CRABP2 was highly expressed in DPCs, which suggested that CRABP2 may influence the number of DPCs. In the present study, we aimed to understand the effect of CRABP2 in Hu sheep dermal papilla cells (DPCs). First, we explored the influence of CRABP2 on the ability of Hu sheep DPCs’ proliferation. Based on the results obtained from some experiments, such as CCK-8, EDU, qPCR, and Western blot experiment, we found that the overexpression of CRABP2 facilitated the proliferation of DPCs compared to the negative control group. Then, we also detected the effect of CRABP2 on the Wnt/β-catenin pathway based on the important function of the Wnt/β-catenin pathway in hair follicles. The results showed that CRABP2 could activate the Wnt/β-catenin pathway in DPCs, and it rescues the proliferation of DPCs when the Wnt/β-catenin pathway was inhibited. In summary, our findings indicate that CRABP2 is a vital functional gene in the proliferation of Hu sheep DPCs. Our study will be of great use for revealing the roles of CRABP2 in the hair follicles of Hu sheep.https://www.mdpi.com/2076-2615/13/12/2033CRABP2DPCsproliferationWnt/β-catenin |
| spellingShingle | Mingliang He Xiaoyang Lv Xiukai Cao Zehu Yuan Kai Quan Tesfaye Getachew Joram M. Mwacharo Aynalem Haile Yutao Li Shanhe Wang Wei Sun CRABP2 Promotes the Proliferation of Dermal Papilla Cells via the Wnt/β-Catenin Pathway Animals CRABP2 DPCs proliferation Wnt/β-catenin |
| title | CRABP2 Promotes the Proliferation of Dermal Papilla Cells via the Wnt/β-Catenin Pathway |
| title_full | CRABP2 Promotes the Proliferation of Dermal Papilla Cells via the Wnt/β-Catenin Pathway |
| title_fullStr | CRABP2 Promotes the Proliferation of Dermal Papilla Cells via the Wnt/β-Catenin Pathway |
| title_full_unstemmed | CRABP2 Promotes the Proliferation of Dermal Papilla Cells via the Wnt/β-Catenin Pathway |
| title_short | CRABP2 Promotes the Proliferation of Dermal Papilla Cells via the Wnt/β-Catenin Pathway |
| title_sort | crabp2 promotes the proliferation of dermal papilla cells via the wnt β catenin pathway |
| topic | CRABP2 DPCs proliferation Wnt/β-catenin |
| url | https://www.mdpi.com/2076-2615/13/12/2033 |
| work_keys_str_mv | AT minglianghe crabp2promotestheproliferationofdermalpapillacellsviathewntbcateninpathway AT xiaoyanglv crabp2promotestheproliferationofdermalpapillacellsviathewntbcateninpathway AT xiukaicao crabp2promotestheproliferationofdermalpapillacellsviathewntbcateninpathway AT zehuyuan crabp2promotestheproliferationofdermalpapillacellsviathewntbcateninpathway AT kaiquan crabp2promotestheproliferationofdermalpapillacellsviathewntbcateninpathway AT tesfayegetachew crabp2promotestheproliferationofdermalpapillacellsviathewntbcateninpathway AT jorammmwacharo crabp2promotestheproliferationofdermalpapillacellsviathewntbcateninpathway AT aynalemhaile crabp2promotestheproliferationofdermalpapillacellsviathewntbcateninpathway AT yutaoli crabp2promotestheproliferationofdermalpapillacellsviathewntbcateninpathway AT shanhewang crabp2promotestheproliferationofdermalpapillacellsviathewntbcateninpathway AT weisun crabp2promotestheproliferationofdermalpapillacellsviathewntbcateninpathway |