Autoantigen Microarray for High-throughput Autoantibody Profiling in Systemic Lupus Erythematosus
Systemic lupus erythematosus (SLE) is a complex autoimmune disease characterized by the production of autoantibodies to a broad range of self-antigens. Profiling the autoantibody repertoire using array-based technology has emerged as a powerful tool for the identification of biomarkers in SLE and ot...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2015-08-01
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| Series: | Genomics, Proteomics & Bioinformatics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672022915001096 |
| _version_ | 1827160790228008960 |
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| author | Honglin Zhu Hui Luo Mei Yan Xiaoxia Zuo Quan-Zhen Li |
| author_facet | Honglin Zhu Hui Luo Mei Yan Xiaoxia Zuo Quan-Zhen Li |
| author_sort | Honglin Zhu |
| collection | DOAJ |
| description | Systemic lupus erythematosus (SLE) is a complex autoimmune disease characterized by the production of autoantibodies to a broad range of self-antigens. Profiling the autoantibody repertoire using array-based technology has emerged as a powerful tool for the identification of biomarkers in SLE and other autoimmune diseases. Proteomic microarray has the capacity to hold large number of self-antigens on a solid surface and serve as a high-throughput screening method for the determination of autoantibody specificities. The autoantigen arrays carrying a wide variety of self-antigens, such as cell nuclear components (nucleic acids and associated proteins), cytoplasmic proteins, phospholipid proteins, cell matrix proteins, mucosal/secreted proteins, glomeruli, and other tissue-specific proteins, have been used for screening of autoantibody specificities associated with different manifestations of SLE. Arrays containing synthetic peptides and molecular modified proteins are also being utilized for identification of autoantibodies targeting to special antigenic epitopes. Different isotypes of autoantibodies, including IgG, IgM, IgA, and IgE, as well as other Ig subtypes, can be detected simultaneously with multi-color labeled secondary antibodies. Serum and plasma are the most common biologic materials for autoantibody detection, but other body fluids such as cerebrospinal fluid, synovial fluid, and saliva can also be a source of autoantibody detection. Proteomic microarray as a multiplexed high-throughput screening platform is playing an increasingly-important role in autoantibody diagnostics. In this article, we highlight the use of autoantigen microarrays for autoantibody exploration in SLE. |
| first_indexed | 2024-03-08T07:18:00Z |
| format | Article |
| id | doaj.art-f0dc7bca08e148edb91e3efbe7a8cdfe |
| institution | Directory Open Access Journal |
| issn | 1672-0229 |
| language | English |
| last_indexed | 2025-03-21T00:18:37Z |
| publishDate | 2015-08-01 |
| publisher | Oxford University Press |
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| series | Genomics, Proteomics & Bioinformatics |
| spelling | doaj.art-f0dc7bca08e148edb91e3efbe7a8cdfe2024-08-03T08:58:43ZengOxford University PressGenomics, Proteomics & Bioinformatics1672-02292015-08-0113421021810.1016/j.gpb.2015.09.001Autoantigen Microarray for High-throughput Autoantibody Profiling in Systemic Lupus ErythematosusHonglin Zhu0Hui Luo1Mei Yan2Xiaoxia Zuo3Quan-Zhen Li4Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Immunology and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75235, USADepartment of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Immunology and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75235, USASystemic lupus erythematosus (SLE) is a complex autoimmune disease characterized by the production of autoantibodies to a broad range of self-antigens. Profiling the autoantibody repertoire using array-based technology has emerged as a powerful tool for the identification of biomarkers in SLE and other autoimmune diseases. Proteomic microarray has the capacity to hold large number of self-antigens on a solid surface and serve as a high-throughput screening method for the determination of autoantibody specificities. The autoantigen arrays carrying a wide variety of self-antigens, such as cell nuclear components (nucleic acids and associated proteins), cytoplasmic proteins, phospholipid proteins, cell matrix proteins, mucosal/secreted proteins, glomeruli, and other tissue-specific proteins, have been used for screening of autoantibody specificities associated with different manifestations of SLE. Arrays containing synthetic peptides and molecular modified proteins are also being utilized for identification of autoantibodies targeting to special antigenic epitopes. Different isotypes of autoantibodies, including IgG, IgM, IgA, and IgE, as well as other Ig subtypes, can be detected simultaneously with multi-color labeled secondary antibodies. Serum and plasma are the most common biologic materials for autoantibody detection, but other body fluids such as cerebrospinal fluid, synovial fluid, and saliva can also be a source of autoantibody detection. Proteomic microarray as a multiplexed high-throughput screening platform is playing an increasingly-important role in autoantibody diagnostics. In this article, we highlight the use of autoantigen microarrays for autoantibody exploration in SLE.http://www.sciencedirect.com/science/article/pii/S1672022915001096Systemic lupus erythematosus (SLE)Autoantibody profilingProteomic microarrayBiomarkerHigh-throughput assay |
| spellingShingle | Honglin Zhu Hui Luo Mei Yan Xiaoxia Zuo Quan-Zhen Li Autoantigen Microarray for High-throughput Autoantibody Profiling in Systemic Lupus Erythematosus Genomics, Proteomics & Bioinformatics Systemic lupus erythematosus (SLE) Autoantibody profiling Proteomic microarray Biomarker High-throughput assay |
| title | Autoantigen Microarray for High-throughput Autoantibody Profiling in Systemic Lupus Erythematosus |
| title_full | Autoantigen Microarray for High-throughput Autoantibody Profiling in Systemic Lupus Erythematosus |
| title_fullStr | Autoantigen Microarray for High-throughput Autoantibody Profiling in Systemic Lupus Erythematosus |
| title_full_unstemmed | Autoantigen Microarray for High-throughput Autoantibody Profiling in Systemic Lupus Erythematosus |
| title_short | Autoantigen Microarray for High-throughput Autoantibody Profiling in Systemic Lupus Erythematosus |
| title_sort | autoantigen microarray for high throughput autoantibody profiling in systemic lupus erythematosus |
| topic | Systemic lupus erythematosus (SLE) Autoantibody profiling Proteomic microarray Biomarker High-throughput assay |
| url | http://www.sciencedirect.com/science/article/pii/S1672022915001096 |
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