Molecular Analysis of Two Endemic <i>Squalius</i> Species: Evidence for Intergeneric Introgression among Cyprinids and Conservation Issues
Conservation of indigenous species, especially endemic ones, is of the utmost importance. Morphological determination of species is usually not sufficient; therefore, molecular phylogenetic analyses of the Illyrian chub, <i>Squalius illyricus,</i> and the Zrmanja chub, <i>Squalius...
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MDPI AG
2023-12-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/9/1/4 |
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| author | Damir Valić Matej Kristan Mirković Višnja Besendorfer Emin Teskeredžić |
| author_facet | Damir Valić Matej Kristan Mirković Višnja Besendorfer Emin Teskeredžić |
| author_sort | Damir Valić |
| collection | DOAJ |
| description | Conservation of indigenous species, especially endemic ones, is of the utmost importance. Morphological determination of species is usually not sufficient; therefore, molecular phylogenetic analyses of the Illyrian chub, <i>Squalius illyricus,</i> and the Zrmanja chub, <i>Squalius zrmanjae,</i> from the Krka River were performed. For the genetic characterization of the mitochondrial gene cytochrome <i>b</i> and the non-coding nuclear region <i>Cyfun P</i>, 15 specimens from each species were subjected to analysis. The obtained sequences were aligned with similar ones from GenBank to determine the taxonomic and phylogenetic position of these species. The obtained molecular results imply that <i>S. zrmanjae</i> from the Krka River has a nuclear region that resembles Dalmatian rudd, <i>Scardinius dergle</i>. This result implies an introgression event and the transfer of genetic information between the two genera. The investigated species are on the IUCN Red List of Threatened Species, their biological data are scarce, and further investigation and protection are needed. |
| first_indexed | 2024-03-08T10:57:45Z |
| format | Article |
| id | doaj.art-f0dc97a1e94e4166b723864b05e62e76 |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-08T10:57:45Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-f0dc97a1e94e4166b723864b05e62e762024-01-26T16:24:21ZengMDPI AGFishes2410-38882023-12-0191410.3390/fishes9010004Molecular Analysis of Two Endemic <i>Squalius</i> Species: Evidence for Intergeneric Introgression among Cyprinids and Conservation IssuesDamir Valić0Matej Kristan Mirković1Višnja Besendorfer2Emin Teskeredžić3Laboratory for Biological Effects of Metals, Division for Marine and Environmental Research, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, CroatiaDepartment of Biology, Faculty of Science, University of Zagreb, Ravnice 48, 10000 Zagreb, CroatiaDivision of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, CroatiaLaboratory for Aquaculture and Pathology of Aquatic Organisms, Division of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, CroatiaConservation of indigenous species, especially endemic ones, is of the utmost importance. Morphological determination of species is usually not sufficient; therefore, molecular phylogenetic analyses of the Illyrian chub, <i>Squalius illyricus,</i> and the Zrmanja chub, <i>Squalius zrmanjae,</i> from the Krka River were performed. For the genetic characterization of the mitochondrial gene cytochrome <i>b</i> and the non-coding nuclear region <i>Cyfun P</i>, 15 specimens from each species were subjected to analysis. The obtained sequences were aligned with similar ones from GenBank to determine the taxonomic and phylogenetic position of these species. The obtained molecular results imply that <i>S. zrmanjae</i> from the Krka River has a nuclear region that resembles Dalmatian rudd, <i>Scardinius dergle</i>. This result implies an introgression event and the transfer of genetic information between the two genera. The investigated species are on the IUCN Red List of Threatened Species, their biological data are scarce, and further investigation and protection are needed.https://www.mdpi.com/2410-3888/9/1/4Leuciscidaecyt <i>b</i>endemic species<i>Squalius</i> |
| spellingShingle | Damir Valić Matej Kristan Mirković Višnja Besendorfer Emin Teskeredžić Molecular Analysis of Two Endemic <i>Squalius</i> Species: Evidence for Intergeneric Introgression among Cyprinids and Conservation Issues Fishes Leuciscidae cyt <i>b</i> endemic species <i>Squalius</i> |
| title | Molecular Analysis of Two Endemic <i>Squalius</i> Species: Evidence for Intergeneric Introgression among Cyprinids and Conservation Issues |
| title_full | Molecular Analysis of Two Endemic <i>Squalius</i> Species: Evidence for Intergeneric Introgression among Cyprinids and Conservation Issues |
| title_fullStr | Molecular Analysis of Two Endemic <i>Squalius</i> Species: Evidence for Intergeneric Introgression among Cyprinids and Conservation Issues |
| title_full_unstemmed | Molecular Analysis of Two Endemic <i>Squalius</i> Species: Evidence for Intergeneric Introgression among Cyprinids and Conservation Issues |
| title_short | Molecular Analysis of Two Endemic <i>Squalius</i> Species: Evidence for Intergeneric Introgression among Cyprinids and Conservation Issues |
| title_sort | molecular analysis of two endemic i squalius i species evidence for intergeneric introgression among cyprinids and conservation issues |
| topic | Leuciscidae cyt <i>b</i> endemic species <i>Squalius</i> |
| url | https://www.mdpi.com/2410-3888/9/1/4 |
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