Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region.
Vibrio parahaemolyticus is an important pathogen causing food-borne disease worldwide. An 80-kb pathogenicity island (Vp-PAI), which contains two tdh (thermostable direct hemolysin) genes and a set of genes for the type III secretion system (T3SS2), is closely related to the pathogenicity of this ba...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2800187?pdf=render |
| _version_ | 1819162752262864896 |
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| author | Toshio Kodama Kazuyoshi Gotoh Hirotaka Hiyoshi Mikiharu Morita Kaori Izutsu Yukihiro Akeda Kwon-Sam Park Vlademir V Cantarelli Rikard Dryselius Tetsuya Iida Takeshi Honda |
| author_facet | Toshio Kodama Kazuyoshi Gotoh Hirotaka Hiyoshi Mikiharu Morita Kaori Izutsu Yukihiro Akeda Kwon-Sam Park Vlademir V Cantarelli Rikard Dryselius Tetsuya Iida Takeshi Honda |
| author_sort | Toshio Kodama |
| collection | DOAJ |
| description | Vibrio parahaemolyticus is an important pathogen causing food-borne disease worldwide. An 80-kb pathogenicity island (Vp-PAI), which contains two tdh (thermostable direct hemolysin) genes and a set of genes for the type III secretion system (T3SS2), is closely related to the pathogenicity of this bacterium. However, the regulatory mechanisms of Vp-PAI's gene expression are poorly understood. Here we report that two novel ToxR-like transcriptional regulatory proteins (VtrA and VtrB) regulate the expression of the genes encoded within the Vp-PAI region, including those for TDH and T3SS2-related proteins. Expression of vtrB was under control of the VtrA, as vector-expressed vtrB was able to recover a functional protein secretory capacity for T3SS2, independent of VtrA. Moreover, these regulatory proteins were essential for T3SS2-dependent biological activities, such as in vitro cytotoxicity and in vivo enterotoxicity. Enterotoxic activities of vtrA and/or vtrB deletion strains derived from the wild-type strain were almost absent, showing fluid accumulation similar to non-infected control. Whole genome transcriptional profiling of vtrA or vtrB deletion strains revealed that the expression levels of over 60 genes were downregulated significantly in these deletion mutant strains and that such genes were almost exclusively located in the Vp-PAI region. These results strongly suggest that VtrA and VtrB are master regulators for virulence gene expression in the Vp-PAI and play critical roles in the pathogenicity of this bacterium. |
| first_indexed | 2024-12-22T17:33:14Z |
| format | Article |
| id | doaj.art-f0de0104afdf4a7eac542bfedc25aa60 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T17:33:14Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f0de0104afdf4a7eac542bfedc25aa602022-12-21T18:18:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-0151e867810.1371/journal.pone.0008678Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region.Toshio KodamaKazuyoshi GotohHirotaka HiyoshiMikiharu MoritaKaori IzutsuYukihiro AkedaKwon-Sam ParkVlademir V CantarelliRikard DryseliusTetsuya IidaTakeshi HondaVibrio parahaemolyticus is an important pathogen causing food-borne disease worldwide. An 80-kb pathogenicity island (Vp-PAI), which contains two tdh (thermostable direct hemolysin) genes and a set of genes for the type III secretion system (T3SS2), is closely related to the pathogenicity of this bacterium. However, the regulatory mechanisms of Vp-PAI's gene expression are poorly understood. Here we report that two novel ToxR-like transcriptional regulatory proteins (VtrA and VtrB) regulate the expression of the genes encoded within the Vp-PAI region, including those for TDH and T3SS2-related proteins. Expression of vtrB was under control of the VtrA, as vector-expressed vtrB was able to recover a functional protein secretory capacity for T3SS2, independent of VtrA. Moreover, these regulatory proteins were essential for T3SS2-dependent biological activities, such as in vitro cytotoxicity and in vivo enterotoxicity. Enterotoxic activities of vtrA and/or vtrB deletion strains derived from the wild-type strain were almost absent, showing fluid accumulation similar to non-infected control. Whole genome transcriptional profiling of vtrA or vtrB deletion strains revealed that the expression levels of over 60 genes were downregulated significantly in these deletion mutant strains and that such genes were almost exclusively located in the Vp-PAI region. These results strongly suggest that VtrA and VtrB are master regulators for virulence gene expression in the Vp-PAI and play critical roles in the pathogenicity of this bacterium.http://europepmc.org/articles/PMC2800187?pdf=render |
| spellingShingle | Toshio Kodama Kazuyoshi Gotoh Hirotaka Hiyoshi Mikiharu Morita Kaori Izutsu Yukihiro Akeda Kwon-Sam Park Vlademir V Cantarelli Rikard Dryselius Tetsuya Iida Takeshi Honda Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region. PLoS ONE |
| title | Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region. |
| title_full | Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region. |
| title_fullStr | Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region. |
| title_full_unstemmed | Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region. |
| title_short | Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region. |
| title_sort | two regulators of vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the vp pai region |
| url | http://europepmc.org/articles/PMC2800187?pdf=render |
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