Silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in Caralluma tuberculata
Elicited plant in vitro cultures are gaining more interest worldwide for their potential in the uniform production of industrially important secondary metabolites. In the present study, different ratios of silver nanoparticles (AgNPs) and plant growth regulators (PGRs) were supplemented to in vitro...
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| Format: | Article |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1577884 |
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| author | Amir Ali Sher Mohammad Mubarak Ali Khan Naveed Iqbal Raja Mohammad Arif Atif Kamil Zia-ur-Rehman Mashwani |
| author_facet | Amir Ali Sher Mohammad Mubarak Ali Khan Naveed Iqbal Raja Mohammad Arif Atif Kamil Zia-ur-Rehman Mashwani |
| author_sort | Amir Ali |
| collection | DOAJ |
| description | Elicited plant in vitro cultures are gaining more interest worldwide for their potential in the uniform production of industrially important secondary metabolites. In the present study, different ratios of silver nanoparticles (AgNPs) and plant growth regulators (PGRs) were supplemented to in vitro cultures for the sustainable production of biomass and antioxidant secondary metabolites through callus cultures of Caralluma tuberculata. Results indicated that various concentrations of AgNPs significantly affected the callus proliferation and substantially increased the callus biomass, when combined with PGRs in the MS (Murashige and Skoog) media. The highest fresh (0.78 g/l) and dry (0.051 g/l) biomass accumulation of callus was observed in the cultures raised in vitro at 60 µg/l AgNPs in combination with 0.5 mg/l 2,4-D plus 3.0 mg/l BA. Phytochemical analysis of the callus cultures showed higher production of phenolics (TPC:3.0 mg), flavonoids (TFC:1.8 mg), phenylalanine ammonialyase activity (PAL: 5.8 U/mg) and antioxidant activity (90%), respectively, in the callus cultures established on MS media in the presence of 90 ug/l AgNPs. Moreover, enhanced activities of antioxidant enzymes such as superoxide dismutase (SOD: 4.8 U/mg), peroxidase (POD: 3.3 U/mg), catalase (CAT: 2.5 U/mg) and ascorbate peroxidase (APX: 1.9 U/mg) were detected at higher level (90 ug/l) of AgNPs tested alone for callus proliferation in the MS media. It may be concluded that the AgNPs can be effectively utilized for the enhancement of bioactive antioxidants in the callus cultures of C. tuberculata, a highly medicinal and threatened plant. This protocol can be scaled up for the industrial production of plant biomass and pharmacologically potent metabolites in C. tuberculata. |
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| format | Article |
| id | doaj.art-f0e0a0e102924189af736f8c68099a26 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-13T23:05:40Z |
| publishDate | 2019-12-01 |
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| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-f0e0a0e102924189af736f8c68099a262022-12-22T02:25:41ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-0147171572410.1080/21691401.2019.1577884Silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in Caralluma tuberculataAmir Ali0Sher Mohammad1Mubarak Ali Khan2Naveed Iqbal Raja3Mohammad Arif4Atif Kamil5Zia-ur-Rehman Mashwani6Department of Botany, PMAS, Arid Agriculture University, Rawalpindi, PakistanBiotechnology Laboratory, Agricultural Research Institute (ARI) Tarnab, Peshawar, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Botany, PMAS, Arid Agriculture University, Rawalpindi, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, PakistanDepartment of Botany, PMAS, Arid Agriculture University, Rawalpindi, PakistanElicited plant in vitro cultures are gaining more interest worldwide for their potential in the uniform production of industrially important secondary metabolites. In the present study, different ratios of silver nanoparticles (AgNPs) and plant growth regulators (PGRs) were supplemented to in vitro cultures for the sustainable production of biomass and antioxidant secondary metabolites through callus cultures of Caralluma tuberculata. Results indicated that various concentrations of AgNPs significantly affected the callus proliferation and substantially increased the callus biomass, when combined with PGRs in the MS (Murashige and Skoog) media. The highest fresh (0.78 g/l) and dry (0.051 g/l) biomass accumulation of callus was observed in the cultures raised in vitro at 60 µg/l AgNPs in combination with 0.5 mg/l 2,4-D plus 3.0 mg/l BA. Phytochemical analysis of the callus cultures showed higher production of phenolics (TPC:3.0 mg), flavonoids (TFC:1.8 mg), phenylalanine ammonialyase activity (PAL: 5.8 U/mg) and antioxidant activity (90%), respectively, in the callus cultures established on MS media in the presence of 90 ug/l AgNPs. Moreover, enhanced activities of antioxidant enzymes such as superoxide dismutase (SOD: 4.8 U/mg), peroxidase (POD: 3.3 U/mg), catalase (CAT: 2.5 U/mg) and ascorbate peroxidase (APX: 1.9 U/mg) were detected at higher level (90 ug/l) of AgNPs tested alone for callus proliferation in the MS media. It may be concluded that the AgNPs can be effectively utilized for the enhancement of bioactive antioxidants in the callus cultures of C. tuberculata, a highly medicinal and threatened plant. This protocol can be scaled up for the industrial production of plant biomass and pharmacologically potent metabolites in C. tuberculata.https://www.tandfonline.com/doi/10.1080/21691401.2019.1577884Silver nanoparticlesplant growth regulatorsCarallumacallus culturesecondary metabolites |
| spellingShingle | Amir Ali Sher Mohammad Mubarak Ali Khan Naveed Iqbal Raja Mohammad Arif Atif Kamil Zia-ur-Rehman Mashwani Silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in Caralluma tuberculata Artificial Cells, Nanomedicine, and Biotechnology Silver nanoparticles plant growth regulators Caralluma callus culture secondary metabolites |
| title | Silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in Caralluma tuberculata |
| title_full | Silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in Caralluma tuberculata |
| title_fullStr | Silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in Caralluma tuberculata |
| title_full_unstemmed | Silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in Caralluma tuberculata |
| title_short | Silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in Caralluma tuberculata |
| title_sort | silver nanoparticles elicited in vitro callus cultures for accumulation of biomass and secondary metabolites in caralluma tuberculata |
| topic | Silver nanoparticles plant growth regulators Caralluma callus culture secondary metabolites |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1577884 |
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