Immunohistochemical (IHC) Analysis of Non-Hodgkin’s Lymphoma (NHL) Spectrum According to WHO/REAL Classification: A Single Centre Experience from Punjab, India
Introduction: The distribution of the major subtypes of non-Hodgkin’s lymphoma (NHL) differs across geographic regions. This study, from the north Indian state of Punjab, has incorporated immunophenotypic findings while investigating the distribution of NHL subtypes based on World Health Organiz...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2014-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/3988/14-8173_F(DK)_PF1(RKH)_PFA(H).pdf |
| Summary: | Introduction: The distribution of the major subtypes of
non-Hodgkin’s lymphoma (NHL) differs across geographic
regions. This study, from the north Indian state of Punjab, has
incorporated immunophenotypic findings while investigating the
distribution of NHL subtypes based on World Health Organization
(WHO)/ Revised European-American Classification of Lymphoid
Neoplasms (REAL) system of classification.
Patients and methods: Over all seventy seven cases of
lymphoma over a period of one year (between April 2012 and April
2013) were diagnosed in the Department of Pathology, Sri Guru
Ram Das Institute of Medical Sciences and Research, Amritsar
(Punjab). Of these 30 cases (39%) were of Hodgkin’s Lymphoma
(HL) and 47 cases (61%) were of Non Hodgkins lymphoma NHL.
Of the total of cases of lympho-proliferative disorders, the
diagnosis of NHL was done by light microscopy alone. All
the cases diagnosed provisionally as NHL were taken up for
immunophenotyping with Immunohistochemical (IHC) studies.
There was 100 % concordance between the light microscopy
and IHC studies.
The individual NHL cases were classified according to the WHO/
REAL classification according to the positive or relevant negative
immonophenotypic expression and tabulated to ascertain the
morphological spectrum of NHL in this part of the country.
Results: B-cell lymphomas formed 89.3%, whereas T-cell
lymphomas formed 10.7% of the NHLs. Diffuse Large B-Cell
Lymphoma (DLBCL) was the most common subtype (46.8%
of all NHLs). B-cell small lymphocytic lymphoma, Mantle-Cell
Lymphoma (MCL), marginal zone B-cell lymphomas (including
MALT lymphomas), Diffuse, mixed small cleaved cell and largecell type and Follicular centre-cell lymphomas amounted to 17%,
12.8%, 2.1%, 2.1% and 4.3%, respectively. Among the T-cell
lymphomas, T-cell lymphoblastic lymphoma, anaplastic largecell lymphomas of T/null-cell type, and Angioimmunoblastic
T-cell lymphoma (AITL) accounted for 6.4%, 2.1%, and 2.1% of
all NHL cases, respectively.
Conclusions: The distribution of NHL subtypes in India shows
disparity with those from the rest of the world. Follicular Lymphoma
(FL) and MCL are less common in India compared to Europe and
the USA. Peripheral T-cell lymphomas and T/NK-cell lymphomas
of nasal and nasal types, which are common in many other Asian
countries, are also less prevalent. T-cell lymphoblastic lymphoma
and anaplastic large T/null cell lymphoma are more prevalent in
India. |
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| ISSN: | 2249-782X 0973-709X |