Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells
Ahmad Abolhasani,1 Davoud Biria,1 Hoda Abolhasani,2 Ali Zarrabi,1 Tahereh Komeili2 1Department of Biotechnology, University of Isfahan, Isfahan, Iran; 2Department of Physiology and Pharmacology, Qom University of Medical Sciences, Qom, IranCorrespondence: Davoud BiriaDepartment of Biotechnology, Uni...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-12-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/investigation-of-the-role-of-glucose-decorated-chitosan-and-plga-nanop-peer-reviewed-article-IJN |
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| author | Abolhasani A Biria D Abolhasani H Zarrabi A Komeili T |
| author_facet | Abolhasani A Biria D Abolhasani H Zarrabi A Komeili T |
| author_sort | Abolhasani A |
| collection | DOAJ |
| description | Ahmad Abolhasani,1 Davoud Biria,1 Hoda Abolhasani,2 Ali Zarrabi,1 Tahereh Komeili2 1Department of Biotechnology, University of Isfahan, Isfahan, Iran; 2Department of Physiology and Pharmacology, Qom University of Medical Sciences, Qom, IranCorrespondence: Davoud BiriaDepartment of Biotechnology, University of Isfahan, P.O. Box: 8174673441, Isfahan, IranTel +983137934373Email d.biria@ast.ui.ac.irPurpose: Glucose decorated PLGA and chitosan nanoparticles (GPNPs and GCNPs) have been developed to examine the possibility of preventing the facilitated glucose transport to the cells through blocking the glucose transporters (Gluts) overexpressed by tumor cells.Methods: The MTT assay was used to assess the cytotoxicity towards human colon tumor (HT-29) cells in 72 hrs. Fluorescence microscopy was employed to confirm the attachment of GPNPs to the cells. Moreover, the GPNPs effects on the apoptotic rate of HT-29 cells were analyzed. Finally, the expression levels of GLUT-1 and GLUT-4 by real-time polymerase chain reaction (RT-PCR) were assayed to investigate the response of HT-29 cells to blocking their Gluts by GPNPs.Results: The stability studies showed that the synthesized complexes were mostly stable (more than 80%) at various temperatures (4 to 40ºC) and pH (5.4 to 7.4) conditions. Results indicated that the survival rate of the cells was decreased to 43% and 46% after treatment with GCNPs and GPNPs, respectively. Also, the apoptosis assay results showed that the percentage of viable cells reduced to 47% after GPNPs treatment. These observations were justified by the specific interactions between the glucose terminals and the cells Gluts which resulted in blocking the entries of nutrients to the cells. It was revealed that the GLUT-1 mRNA expression after the first 24 h of treatment by GPNPs was upregulated to more than 145%, while the direction was reversed after 72 h (expression less than 45%), which coincided with the cells death. In the first 24 h, the glucose deprivation stimulated the expression of Glut-1 while the apoptotic enzymes expression was dominant at the end of 72 h treatment time.Conclusion: Finally, it can be concluded that the glucose-nanoparticle complexes could be considered as promising agents in cancer therapy.Keywords: glucose decorated nanoparticles, PLGA, chitosan, glut, tumor cells |
| first_indexed | 2024-12-14T13:48:36Z |
| format | Article |
| id | doaj.art-f0e51301b70743dda926145ca2cb27d4 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-14T13:48:36Z |
| publishDate | 2019-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-f0e51301b70743dda926145ca2cb27d42022-12-21T22:59:14ZengDove Medical PressInternational Journal of Nanomedicine1178-20132019-12-01Volume 149535954650221Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor CellsAbolhasani ABiria DAbolhasani HZarrabi AKomeili TAhmad Abolhasani,1 Davoud Biria,1 Hoda Abolhasani,2 Ali Zarrabi,1 Tahereh Komeili2 1Department of Biotechnology, University of Isfahan, Isfahan, Iran; 2Department of Physiology and Pharmacology, Qom University of Medical Sciences, Qom, IranCorrespondence: Davoud BiriaDepartment of Biotechnology, University of Isfahan, P.O. Box: 8174673441, Isfahan, IranTel +983137934373Email d.biria@ast.ui.ac.irPurpose: Glucose decorated PLGA and chitosan nanoparticles (GPNPs and GCNPs) have been developed to examine the possibility of preventing the facilitated glucose transport to the cells through blocking the glucose transporters (Gluts) overexpressed by tumor cells.Methods: The MTT assay was used to assess the cytotoxicity towards human colon tumor (HT-29) cells in 72 hrs. Fluorescence microscopy was employed to confirm the attachment of GPNPs to the cells. Moreover, the GPNPs effects on the apoptotic rate of HT-29 cells were analyzed. Finally, the expression levels of GLUT-1 and GLUT-4 by real-time polymerase chain reaction (RT-PCR) were assayed to investigate the response of HT-29 cells to blocking their Gluts by GPNPs.Results: The stability studies showed that the synthesized complexes were mostly stable (more than 80%) at various temperatures (4 to 40ºC) and pH (5.4 to 7.4) conditions. Results indicated that the survival rate of the cells was decreased to 43% and 46% after treatment with GCNPs and GPNPs, respectively. Also, the apoptosis assay results showed that the percentage of viable cells reduced to 47% after GPNPs treatment. These observations were justified by the specific interactions between the glucose terminals and the cells Gluts which resulted in blocking the entries of nutrients to the cells. It was revealed that the GLUT-1 mRNA expression after the first 24 h of treatment by GPNPs was upregulated to more than 145%, while the direction was reversed after 72 h (expression less than 45%), which coincided with the cells death. In the first 24 h, the glucose deprivation stimulated the expression of Glut-1 while the apoptotic enzymes expression was dominant at the end of 72 h treatment time.Conclusion: Finally, it can be concluded that the glucose-nanoparticle complexes could be considered as promising agents in cancer therapy.Keywords: glucose decorated nanoparticles, PLGA, chitosan, glut, tumor cellshttps://www.dovepress.com/investigation-of-the-role-of-glucose-decorated-chitosan-and-plga-nanop-peer-reviewed-article-IJNglucose decorated nanoparticlesplgachitosangluttumor cells. |
| spellingShingle | Abolhasani A Biria D Abolhasani H Zarrabi A Komeili T Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells International Journal of Nanomedicine glucose decorated nanoparticles plga chitosan glut tumor cells. |
| title | Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells |
| title_full | Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells |
| title_fullStr | Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells |
| title_full_unstemmed | Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells |
| title_short | Investigation of the Role of Glucose Decorated Chitosan and PLGA Nanoparticles as Blocking Agents to Glucose Transporters of Tumor Cells |
| title_sort | investigation of the role of glucose decorated chitosan and plga nanoparticles as blocking agents to glucose transporters of tumor cells |
| topic | glucose decorated nanoparticles plga chitosan glut tumor cells. |
| url | https://www.dovepress.com/investigation-of-the-role-of-glucose-decorated-chitosan-and-plga-nanop-peer-reviewed-article-IJN |
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