A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization
There is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of gold nanoparticle-based multilayer nanoconstructs...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/11/2775 |
| _version_ | 1797509123536846848 |
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| author | Anna V. Epanchintseva Julia E. Poletaeva Ilya S. Dovydenko Boris P. Chelobanov Dmitrii V. Pyshnyi Elena I. Ryabchikova Inna A. Pyshnaya |
| author_facet | Anna V. Epanchintseva Julia E. Poletaeva Ilya S. Dovydenko Boris P. Chelobanov Dmitrii V. Pyshnyi Elena I. Ryabchikova Inna A. Pyshnaya |
| author_sort | Anna V. Epanchintseva |
| collection | DOAJ |
| description | There is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of gold nanoparticle-based multilayer nanoconstructs (MLNCs) for suppressing reporter protein synthesis. The present work is aimed at improving the quality of preparations of desired MLNCs, and for this purpose, optimal conditions for their multistep fabrication were found. All steps of this process and MLNC purification were verified using dynamic light scattering, transmission electron microscopy, and UV-Vis spectroscopy. Factors influencing the efficiency of nanocomposite assembly, colloidal stability, and purification quality were identified. These data made it possible to optimize the fabrication of target MLNCs bearing small interfering RNA and to substantially improve end product quality via an increase in its homogeneity and a decrease in the amount of incomplete nanoconstructs. We believe that the proposed approaches and methods will be useful for researchers working with lipid nanoconstructs. |
| first_indexed | 2024-03-10T05:13:53Z |
| format | Article |
| id | doaj.art-f0e5ea8054c849ac95ad2f10fdc5d8b2 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T05:13:53Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-f0e5ea8054c849ac95ad2f10fdc5d8b22023-11-23T00:38:11ZengMDPI AGNanomaterials2079-49912021-10-011111277510.3390/nano11112775A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and CharacterizationAnna V. Epanchintseva0Julia E. Poletaeva1Ilya S. Dovydenko2Boris P. Chelobanov3Dmitrii V. Pyshnyi4Elena I. Ryabchikova5Inna A. Pyshnaya6Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaThere is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of gold nanoparticle-based multilayer nanoconstructs (MLNCs) for suppressing reporter protein synthesis. The present work is aimed at improving the quality of preparations of desired MLNCs, and for this purpose, optimal conditions for their multistep fabrication were found. All steps of this process and MLNC purification were verified using dynamic light scattering, transmission electron microscopy, and UV-Vis spectroscopy. Factors influencing the efficiency of nanocomposite assembly, colloidal stability, and purification quality were identified. These data made it possible to optimize the fabrication of target MLNCs bearing small interfering RNA and to substantially improve end product quality via an increase in its homogeneity and a decrease in the amount of incomplete nanoconstructs. We believe that the proposed approaches and methods will be useful for researchers working with lipid nanoconstructs.https://www.mdpi.com/2079-4991/11/11/2775gold nanoparticlessiRNAnoncovalent adsorptionlipid envelopingmultilayer nanoconstructs for siRNA preparation and purification |
| spellingShingle | Anna V. Epanchintseva Julia E. Poletaeva Ilya S. Dovydenko Boris P. Chelobanov Dmitrii V. Pyshnyi Elena I. Ryabchikova Inna A. Pyshnaya A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization Nanomaterials gold nanoparticles siRNA noncovalent adsorption lipid enveloping multilayer nanoconstructs for siRNA preparation and purification |
| title | A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization |
| title_full | A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization |
| title_fullStr | A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization |
| title_full_unstemmed | A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization |
| title_short | A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization |
| title_sort | lipid coated nanoconstruct composed of gold nanoparticles noncovalently coated with small interfering rna preparation purification and characterization |
| topic | gold nanoparticles siRNA noncovalent adsorption lipid enveloping multilayer nanoconstructs for siRNA preparation and purification |
| url | https://www.mdpi.com/2079-4991/11/11/2775 |
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