Effects of Bacillus subtilis and Pseudomonas fluorescens as the soil amendment
The application of soil beneficial bacteria (SBB) in agriculture is steadily increasing as it provides a promising way to replace chemical fertilisers and other supplements. Although the role of SBB as a biofertiliser is well understood, little is known about the response of soil physiochemical prop...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-11-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022029620 |
| _version_ | 1811213803138842624 |
|---|---|
| author | Charles Wang Wai NG Wen Hui YAN Karl Wah Keung TSIM Pui San SO Yi Teng XIA Chun Ting TO |
| author_facet | Charles Wang Wai NG Wen Hui YAN Karl Wah Keung TSIM Pui San SO Yi Teng XIA Chun Ting TO |
| author_sort | Charles Wang Wai NG |
| collection | DOAJ |
| description | The application of soil beneficial bacteria (SBB) in agriculture is steadily increasing as it provides a promising way to replace chemical fertilisers and other supplements. Although the role of SBB as a biofertiliser is well understood, little is known about the response of soil physiochemical properties via the change in soil enzymatic activities with SBB growth. In this study, sterilised bulk soil was inoculated with Bacillus subtilis (BS) and Pseudomonas fluorescens (PF), which exhibit excellent characteristics in vitro for potentially improving soil quality. It is found that the contents of bioavailable nitrogen and ammonium in soil inoculated with SBB increased significantly, up to 34% and 57% relative to a control. This resulted from the enhancement of soil urease activity with BS and PF treatments by approximately 90% and 70%, respectively. The increased soil urease activity can be explained by the increased microorganism activity evident from the larger population size of BS (0.78–0.97 CFU mL−1/CFU mL−1) than PF (0.55–0.79 CFU mL−1/CFU mL−1) (p < 0.05). Results of principal component analysis also reinforce the interaction apparent in the significant relationship between soil urease activity and microbial biomass carbon (p < 0.05). Therefore, it can be concluded that the enhancement of soil enzymatic activities induced bulk soil fertility upregulation because of bacterial growth. These results demonstrate the application of SBB to be a promising strategy for bulk soil amendment, particularly nutrient restoration. |
| first_indexed | 2024-04-12T05:52:20Z |
| format | Article |
| id | doaj.art-f0e75f4a954944a3821a66088e6725fa |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-12T05:52:20Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-f0e75f4a954944a3821a66088e6725fa2022-12-22T03:45:17ZengElsevierHeliyon2405-84402022-11-01811e11674Effects of Bacillus subtilis and Pseudomonas fluorescens as the soil amendmentCharles Wang Wai NG0Wen Hui YAN1Karl Wah Keung TSIM2Pui San SO3Yi Teng XIA4Chun Ting TO5Department of Civil and Environmental Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, ChinaDepartment of Civil and Environmental Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, China; Corresponding author.Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, ChinaDepartment of Civil and Environmental Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, ChinaDivision of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, ChinaDepartment of Civil and Environmental Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, ChinaThe application of soil beneficial bacteria (SBB) in agriculture is steadily increasing as it provides a promising way to replace chemical fertilisers and other supplements. Although the role of SBB as a biofertiliser is well understood, little is known about the response of soil physiochemical properties via the change in soil enzymatic activities with SBB growth. In this study, sterilised bulk soil was inoculated with Bacillus subtilis (BS) and Pseudomonas fluorescens (PF), which exhibit excellent characteristics in vitro for potentially improving soil quality. It is found that the contents of bioavailable nitrogen and ammonium in soil inoculated with SBB increased significantly, up to 34% and 57% relative to a control. This resulted from the enhancement of soil urease activity with BS and PF treatments by approximately 90% and 70%, respectively. The increased soil urease activity can be explained by the increased microorganism activity evident from the larger population size of BS (0.78–0.97 CFU mL−1/CFU mL−1) than PF (0.55–0.79 CFU mL−1/CFU mL−1) (p < 0.05). Results of principal component analysis also reinforce the interaction apparent in the significant relationship between soil urease activity and microbial biomass carbon (p < 0.05). Therefore, it can be concluded that the enhancement of soil enzymatic activities induced bulk soil fertility upregulation because of bacterial growth. These results demonstrate the application of SBB to be a promising strategy for bulk soil amendment, particularly nutrient restoration.http://www.sciencedirect.com/science/article/pii/S2405844022029620Soil beneficial bacteriaBacillus subtilisPseudomonas fluorescensSoil fertilitySoil urease |
| spellingShingle | Charles Wang Wai NG Wen Hui YAN Karl Wah Keung TSIM Pui San SO Yi Teng XIA Chun Ting TO Effects of Bacillus subtilis and Pseudomonas fluorescens as the soil amendment Heliyon Soil beneficial bacteria Bacillus subtilis Pseudomonas fluorescens Soil fertility Soil urease |
| title | Effects of Bacillus subtilis and Pseudomonas fluorescens as the soil amendment |
| title_full | Effects of Bacillus subtilis and Pseudomonas fluorescens as the soil amendment |
| title_fullStr | Effects of Bacillus subtilis and Pseudomonas fluorescens as the soil amendment |
| title_full_unstemmed | Effects of Bacillus subtilis and Pseudomonas fluorescens as the soil amendment |
| title_short | Effects of Bacillus subtilis and Pseudomonas fluorescens as the soil amendment |
| title_sort | effects of bacillus subtilis and pseudomonas fluorescens as the soil amendment |
| topic | Soil beneficial bacteria Bacillus subtilis Pseudomonas fluorescens Soil fertility Soil urease |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022029620 |
| work_keys_str_mv | AT charleswangwaing effectsofbacillussubtilisandpseudomonasfluorescensasthesoilamendment AT wenhuiyan effectsofbacillussubtilisandpseudomonasfluorescensasthesoilamendment AT karlwahkeungtsim effectsofbacillussubtilisandpseudomonasfluorescensasthesoilamendment AT puisanso effectsofbacillussubtilisandpseudomonasfluorescensasthesoilamendment AT yitengxia effectsofbacillussubtilisandpseudomonasfluorescensasthesoilamendment AT chuntingto effectsofbacillussubtilisandpseudomonasfluorescensasthesoilamendment |