Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.
The phytohormone abscisic acid (ABA) regulates plant growth, development and responses to biotic and abiotic stresses. The core ABA signaling pathway consists of three major components: ABA receptor (PYR1/PYLs), type 2C Protein Phosphatase (PP2C) and SNF1-related protein kinase 2 (SnRK2). Neverthele...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2016-03-01
|
Series: | PLoS Genetics |
Online Access: | http://europepmc.org/articles/PMC4778861?pdf=render |
_version_ | 1818194334768431104 |
---|---|
author | Yueh-Ju Hou Yingfang Zhu Pengcheng Wang Yang Zhao Shaojun Xie Giorgia Batelli Bangshing Wang Cheng-Guo Duan Xingang Wang Lu Xing Mingguang Lei Jun Yan Xiaohong Zhu Jian-Kang Zhu |
author_facet | Yueh-Ju Hou Yingfang Zhu Pengcheng Wang Yang Zhao Shaojun Xie Giorgia Batelli Bangshing Wang Cheng-Guo Duan Xingang Wang Lu Xing Mingguang Lei Jun Yan Xiaohong Zhu Jian-Kang Zhu |
author_sort | Yueh-Ju Hou |
collection | DOAJ |
description | The phytohormone abscisic acid (ABA) regulates plant growth, development and responses to biotic and abiotic stresses. The core ABA signaling pathway consists of three major components: ABA receptor (PYR1/PYLs), type 2C Protein Phosphatase (PP2C) and SNF1-related protein kinase 2 (SnRK2). Nevertheless, the complexity of ABA signaling remains to be explored. To uncover new components of ABA signal transduction pathways, we performed a yeast two-hybrid screen for SnRK2-interacting proteins. We found that Type One Protein Phosphatase 1 (TOPP1) and its regulatory protein, At Inhibitor-2 (AtI-2), physically interact with SnRK2s and also with PYLs. TOPP1 inhibited the kinase activity of SnRK2.6, and this inhibition could be enhanced by AtI-2. Transactivation assays showed that TOPP1 and AtI-2 negatively regulated the SnRK2.2/3/6-mediated activation of the ABA responsive reporter gene RD29B, supporting a negative role of TOPP1 and AtI-2 in ABA signaling. Consistent with these findings, topp1 and ati-2 mutant plants displayed hypersensitivities to ABA and salt treatments, and transcriptome analysis of TOPP1 and AtI-2 knockout plants revealed an increased expression of multiple ABA-responsive genes in the mutants. Taken together, our results uncover TOPP1 and AtI-2 as negative regulators of ABA signaling. |
first_indexed | 2024-12-12T01:00:39Z |
format | Article |
id | doaj.art-f0e76491f921422a8a2d5d805633e270 |
institution | Directory Open Access Journal |
issn | 1553-7390 1553-7404 |
language | English |
last_indexed | 2024-12-12T01:00:39Z |
publishDate | 2016-03-01 |
publisher | Public Library of Science (PLoS) |
record_format | Article |
series | PLoS Genetics |
spelling | doaj.art-f0e76491f921422a8a2d5d805633e2702022-12-22T00:43:44ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042016-03-01123e100583510.1371/journal.pgen.1005835Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.Yueh-Ju HouYingfang ZhuPengcheng WangYang ZhaoShaojun XieGiorgia BatelliBangshing WangCheng-Guo DuanXingang WangLu XingMingguang LeiJun YanXiaohong ZhuJian-Kang ZhuThe phytohormone abscisic acid (ABA) regulates plant growth, development and responses to biotic and abiotic stresses. The core ABA signaling pathway consists of three major components: ABA receptor (PYR1/PYLs), type 2C Protein Phosphatase (PP2C) and SNF1-related protein kinase 2 (SnRK2). Nevertheless, the complexity of ABA signaling remains to be explored. To uncover new components of ABA signal transduction pathways, we performed a yeast two-hybrid screen for SnRK2-interacting proteins. We found that Type One Protein Phosphatase 1 (TOPP1) and its regulatory protein, At Inhibitor-2 (AtI-2), physically interact with SnRK2s and also with PYLs. TOPP1 inhibited the kinase activity of SnRK2.6, and this inhibition could be enhanced by AtI-2. Transactivation assays showed that TOPP1 and AtI-2 negatively regulated the SnRK2.2/3/6-mediated activation of the ABA responsive reporter gene RD29B, supporting a negative role of TOPP1 and AtI-2 in ABA signaling. Consistent with these findings, topp1 and ati-2 mutant plants displayed hypersensitivities to ABA and salt treatments, and transcriptome analysis of TOPP1 and AtI-2 knockout plants revealed an increased expression of multiple ABA-responsive genes in the mutants. Taken together, our results uncover TOPP1 and AtI-2 as negative regulators of ABA signaling.http://europepmc.org/articles/PMC4778861?pdf=render |
spellingShingle | Yueh-Ju Hou Yingfang Zhu Pengcheng Wang Yang Zhao Shaojun Xie Giorgia Batelli Bangshing Wang Cheng-Guo Duan Xingang Wang Lu Xing Mingguang Lei Jun Yan Xiaohong Zhu Jian-Kang Zhu Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling. PLoS Genetics |
title | Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling. |
title_full | Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling. |
title_fullStr | Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling. |
title_full_unstemmed | Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling. |
title_short | Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling. |
title_sort | type one protein phosphatase 1 and its regulatory protein inhibitor 2 negatively regulate aba signaling |
url | http://europepmc.org/articles/PMC4778861?pdf=render |
work_keys_str_mv | AT yuehjuhou typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT yingfangzhu typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT pengchengwang typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT yangzhao typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT shaojunxie typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT giorgiabatelli typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT bangshingwang typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT chengguoduan typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT xingangwang typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT luxing typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT mingguanglei typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT junyan typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT xiaohongzhu typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling AT jiankangzhu typeoneproteinphosphatase1anditsregulatoryproteininhibitor2negativelyregulateabasignaling |