Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.

The phytohormone abscisic acid (ABA) regulates plant growth, development and responses to biotic and abiotic stresses. The core ABA signaling pathway consists of three major components: ABA receptor (PYR1/PYLs), type 2C Protein Phosphatase (PP2C) and SNF1-related protein kinase 2 (SnRK2). Neverthele...

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Main Authors: Yueh-Ju Hou, Yingfang Zhu, Pengcheng Wang, Yang Zhao, Shaojun Xie, Giorgia Batelli, Bangshing Wang, Cheng-Guo Duan, Xingang Wang, Lu Xing, Mingguang Lei, Jun Yan, Xiaohong Zhu, Jian-Kang Zhu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-03-01
Series:PLoS Genetics
Online Access:http://europepmc.org/articles/PMC4778861?pdf=render
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author Yueh-Ju Hou
Yingfang Zhu
Pengcheng Wang
Yang Zhao
Shaojun Xie
Giorgia Batelli
Bangshing Wang
Cheng-Guo Duan
Xingang Wang
Lu Xing
Mingguang Lei
Jun Yan
Xiaohong Zhu
Jian-Kang Zhu
author_facet Yueh-Ju Hou
Yingfang Zhu
Pengcheng Wang
Yang Zhao
Shaojun Xie
Giorgia Batelli
Bangshing Wang
Cheng-Guo Duan
Xingang Wang
Lu Xing
Mingguang Lei
Jun Yan
Xiaohong Zhu
Jian-Kang Zhu
author_sort Yueh-Ju Hou
collection DOAJ
description The phytohormone abscisic acid (ABA) regulates plant growth, development and responses to biotic and abiotic stresses. The core ABA signaling pathway consists of three major components: ABA receptor (PYR1/PYLs), type 2C Protein Phosphatase (PP2C) and SNF1-related protein kinase 2 (SnRK2). Nevertheless, the complexity of ABA signaling remains to be explored. To uncover new components of ABA signal transduction pathways, we performed a yeast two-hybrid screen for SnRK2-interacting proteins. We found that Type One Protein Phosphatase 1 (TOPP1) and its regulatory protein, At Inhibitor-2 (AtI-2), physically interact with SnRK2s and also with PYLs. TOPP1 inhibited the kinase activity of SnRK2.6, and this inhibition could be enhanced by AtI-2. Transactivation assays showed that TOPP1 and AtI-2 negatively regulated the SnRK2.2/3/6-mediated activation of the ABA responsive reporter gene RD29B, supporting a negative role of TOPP1 and AtI-2 in ABA signaling. Consistent with these findings, topp1 and ati-2 mutant plants displayed hypersensitivities to ABA and salt treatments, and transcriptome analysis of TOPP1 and AtI-2 knockout plants revealed an increased expression of multiple ABA-responsive genes in the mutants. Taken together, our results uncover TOPP1 and AtI-2 as negative regulators of ABA signaling.
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spelling doaj.art-f0e76491f921422a8a2d5d805633e2702022-12-22T00:43:44ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042016-03-01123e100583510.1371/journal.pgen.1005835Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.Yueh-Ju HouYingfang ZhuPengcheng WangYang ZhaoShaojun XieGiorgia BatelliBangshing WangCheng-Guo DuanXingang WangLu XingMingguang LeiJun YanXiaohong ZhuJian-Kang ZhuThe phytohormone abscisic acid (ABA) regulates plant growth, development and responses to biotic and abiotic stresses. The core ABA signaling pathway consists of three major components: ABA receptor (PYR1/PYLs), type 2C Protein Phosphatase (PP2C) and SNF1-related protein kinase 2 (SnRK2). Nevertheless, the complexity of ABA signaling remains to be explored. To uncover new components of ABA signal transduction pathways, we performed a yeast two-hybrid screen for SnRK2-interacting proteins. We found that Type One Protein Phosphatase 1 (TOPP1) and its regulatory protein, At Inhibitor-2 (AtI-2), physically interact with SnRK2s and also with PYLs. TOPP1 inhibited the kinase activity of SnRK2.6, and this inhibition could be enhanced by AtI-2. Transactivation assays showed that TOPP1 and AtI-2 negatively regulated the SnRK2.2/3/6-mediated activation of the ABA responsive reporter gene RD29B, supporting a negative role of TOPP1 and AtI-2 in ABA signaling. Consistent with these findings, topp1 and ati-2 mutant plants displayed hypersensitivities to ABA and salt treatments, and transcriptome analysis of TOPP1 and AtI-2 knockout plants revealed an increased expression of multiple ABA-responsive genes in the mutants. Taken together, our results uncover TOPP1 and AtI-2 as negative regulators of ABA signaling.http://europepmc.org/articles/PMC4778861?pdf=render
spellingShingle Yueh-Ju Hou
Yingfang Zhu
Pengcheng Wang
Yang Zhao
Shaojun Xie
Giorgia Batelli
Bangshing Wang
Cheng-Guo Duan
Xingang Wang
Lu Xing
Mingguang Lei
Jun Yan
Xiaohong Zhu
Jian-Kang Zhu
Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.
PLoS Genetics
title Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.
title_full Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.
title_fullStr Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.
title_full_unstemmed Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.
title_short Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.
title_sort type one protein phosphatase 1 and its regulatory protein inhibitor 2 negatively regulate aba signaling
url http://europepmc.org/articles/PMC4778861?pdf=render
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