A method for intracerebroventricular cannulation of young broiler chicks
The site-specific administration of neuroactive substances or pharmacological agents is a routine practice in neurological studies. To facilitate chronic treatments intercranial cannulae are often implanted in the skull. These surgical procedures are widely described and well-documented for rodents,...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2022-01-01
|
Series: | MethodsX |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016122001054 |
_version_ | 1811187293425238016 |
---|---|
author | Chris Lamberigts Zhigang Song Johan Buyse |
author_facet | Chris Lamberigts Zhigang Song Johan Buyse |
author_sort | Chris Lamberigts |
collection | DOAJ |
description | The site-specific administration of neuroactive substances or pharmacological agents is a routine practice in neurological studies. To facilitate chronic treatments intercranial cannulae are often implanted in the skull. These surgical procedures are widely described and well-documented for rodents, as the most common animal model, and even refined over the years. However, precise descriptions of proper procedure for third ventricle cannulation in young broiler chicks is not readily available. This absence is surprising, as chicken models are often used in the investigation of physiological control of the metabolism and ingestion. Furthermore, as a commercial animal, there is also a particular interest in elucidating the central regulation of feed intake and metabolism to optimize feeding and living conditions for broilers. The brain nuclei that serve as the regulatory centers of feeding and metabolism are the arcuate nucleus, the ventromedial hypothalamic nucleus, and the lateral hypothalamus, which are all located near the third ventricle. Here, we provide a full protocol for intracerebroventricular (i.c.v.) cannulation of 7-day old broiler chicks, as well as a review of some of the advances made in rodent i.c.v. cannulations and whether these advances are applicable to cannulations in chickens. Using the surgical procedure described here, we were able to achieve a success rate of proper implantation of 88%, with a mortality rate of less than 1% (n = 224). • Detailed procedure for i.c.v. canula implantation in the third ventricle of 7d-old broiler chicks. • Cement cap with anchoring screws is necessary in broilers, glue does not provide sufficient stability. • Carboxylate cement and self-adhesive resin cement were tested as an alternative for methyl methacrylate cement. |
first_indexed | 2024-04-11T14:00:56Z |
format | Article |
id | doaj.art-f0ed96bb2e46473e927968158e71d76e |
institution | Directory Open Access Journal |
issn | 2215-0161 |
language | English |
last_indexed | 2024-04-11T14:00:56Z |
publishDate | 2022-01-01 |
publisher | Elsevier |
record_format | Article |
series | MethodsX |
spelling | doaj.art-f0ed96bb2e46473e927968158e71d76e2022-12-22T04:20:08ZengElsevierMethodsX2215-01612022-01-019101724A method for intracerebroventricular cannulation of young broiler chicksChris Lamberigts0Zhigang Song1Johan Buyse2Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, Leuven 3001, Belgium; Corresponding author.Department of Animal Science, Shandong Agricultural University, 61 Daizong Street, Taian, ChinaLaboratory of Livestock Physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, Leuven 3001, BelgiumThe site-specific administration of neuroactive substances or pharmacological agents is a routine practice in neurological studies. To facilitate chronic treatments intercranial cannulae are often implanted in the skull. These surgical procedures are widely described and well-documented for rodents, as the most common animal model, and even refined over the years. However, precise descriptions of proper procedure for third ventricle cannulation in young broiler chicks is not readily available. This absence is surprising, as chicken models are often used in the investigation of physiological control of the metabolism and ingestion. Furthermore, as a commercial animal, there is also a particular interest in elucidating the central regulation of feed intake and metabolism to optimize feeding and living conditions for broilers. The brain nuclei that serve as the regulatory centers of feeding and metabolism are the arcuate nucleus, the ventromedial hypothalamic nucleus, and the lateral hypothalamus, which are all located near the third ventricle. Here, we provide a full protocol for intracerebroventricular (i.c.v.) cannulation of 7-day old broiler chicks, as well as a review of some of the advances made in rodent i.c.v. cannulations and whether these advances are applicable to cannulations in chickens. Using the surgical procedure described here, we were able to achieve a success rate of proper implantation of 88%, with a mortality rate of less than 1% (n = 224). • Detailed procedure for i.c.v. canula implantation in the third ventricle of 7d-old broiler chicks. • Cement cap with anchoring screws is necessary in broilers, glue does not provide sufficient stability. • Carboxylate cement and self-adhesive resin cement were tested as an alternative for methyl methacrylate cement.http://www.sciencedirect.com/science/article/pii/S2215016122001054Stereotaxic Implantation of an Intracerebroventricular Cannula |
spellingShingle | Chris Lamberigts Zhigang Song Johan Buyse A method for intracerebroventricular cannulation of young broiler chicks MethodsX Stereotaxic Implantation of an Intracerebroventricular Cannula |
title | A method for intracerebroventricular cannulation of young broiler chicks |
title_full | A method for intracerebroventricular cannulation of young broiler chicks |
title_fullStr | A method for intracerebroventricular cannulation of young broiler chicks |
title_full_unstemmed | A method for intracerebroventricular cannulation of young broiler chicks |
title_short | A method for intracerebroventricular cannulation of young broiler chicks |
title_sort | method for intracerebroventricular cannulation of young broiler chicks |
topic | Stereotaxic Implantation of an Intracerebroventricular Cannula |
url | http://www.sciencedirect.com/science/article/pii/S2215016122001054 |
work_keys_str_mv | AT chrislamberigts amethodforintracerebroventricularcannulationofyoungbroilerchicks AT zhigangsong amethodforintracerebroventricularcannulationofyoungbroilerchicks AT johanbuyse amethodforintracerebroventricularcannulationofyoungbroilerchicks AT chrislamberigts methodforintracerebroventricularcannulationofyoungbroilerchicks AT zhigangsong methodforintracerebroventricularcannulationofyoungbroilerchicks AT johanbuyse methodforintracerebroventricularcannulationofyoungbroilerchicks |