A method for intracerebroventricular cannulation of young broiler chicks

The site-specific administration of neuroactive substances or pharmacological agents is a routine practice in neurological studies. To facilitate chronic treatments intercranial cannulae are often implanted in the skull. These surgical procedures are widely described and well-documented for rodents,...

Full description

Bibliographic Details
Main Authors: Chris Lamberigts, Zhigang Song, Johan Buyse
Format: Article
Language:English
Published: Elsevier 2022-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016122001054
_version_ 1811187293425238016
author Chris Lamberigts
Zhigang Song
Johan Buyse
author_facet Chris Lamberigts
Zhigang Song
Johan Buyse
author_sort Chris Lamberigts
collection DOAJ
description The site-specific administration of neuroactive substances or pharmacological agents is a routine practice in neurological studies. To facilitate chronic treatments intercranial cannulae are often implanted in the skull. These surgical procedures are widely described and well-documented for rodents, as the most common animal model, and even refined over the years. However, precise descriptions of proper procedure for third ventricle cannulation in young broiler chicks is not readily available. This absence is surprising, as chicken models are often used in the investigation of physiological control of the metabolism and ingestion. Furthermore, as a commercial animal, there is also a particular interest in elucidating the central regulation of feed intake and metabolism to optimize feeding and living conditions for broilers. The brain nuclei that serve as the regulatory centers of feeding and metabolism are the arcuate nucleus, the ventromedial hypothalamic nucleus, and the lateral hypothalamus, which are all located near the third ventricle. Here, we provide a full protocol for intracerebroventricular (i.c.v.) cannulation of 7-day old broiler chicks, as well as a review of some of the advances made in rodent i.c.v. cannulations and whether these advances are applicable to cannulations in chickens. Using the surgical procedure described here, we were able to achieve a success rate of proper implantation of 88%, with a mortality rate of less than 1% (n = 224). • Detailed procedure for i.c.v. canula implantation in the third ventricle of 7d-old broiler chicks. • Cement cap with anchoring screws is necessary in broilers, glue does not provide sufficient stability. • Carboxylate cement and self-adhesive resin cement were tested as an alternative for methyl methacrylate cement.
first_indexed 2024-04-11T14:00:56Z
format Article
id doaj.art-f0ed96bb2e46473e927968158e71d76e
institution Directory Open Access Journal
issn 2215-0161
language English
last_indexed 2024-04-11T14:00:56Z
publishDate 2022-01-01
publisher Elsevier
record_format Article
series MethodsX
spelling doaj.art-f0ed96bb2e46473e927968158e71d76e2022-12-22T04:20:08ZengElsevierMethodsX2215-01612022-01-019101724A method for intracerebroventricular cannulation of young broiler chicksChris Lamberigts0Zhigang Song1Johan Buyse2Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, Leuven 3001, Belgium; Corresponding author.Department of Animal Science, Shandong Agricultural University, 61 Daizong Street, Taian, ChinaLaboratory of Livestock Physiology, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, Leuven 3001, BelgiumThe site-specific administration of neuroactive substances or pharmacological agents is a routine practice in neurological studies. To facilitate chronic treatments intercranial cannulae are often implanted in the skull. These surgical procedures are widely described and well-documented for rodents, as the most common animal model, and even refined over the years. However, precise descriptions of proper procedure for third ventricle cannulation in young broiler chicks is not readily available. This absence is surprising, as chicken models are often used in the investigation of physiological control of the metabolism and ingestion. Furthermore, as a commercial animal, there is also a particular interest in elucidating the central regulation of feed intake and metabolism to optimize feeding and living conditions for broilers. The brain nuclei that serve as the regulatory centers of feeding and metabolism are the arcuate nucleus, the ventromedial hypothalamic nucleus, and the lateral hypothalamus, which are all located near the third ventricle. Here, we provide a full protocol for intracerebroventricular (i.c.v.) cannulation of 7-day old broiler chicks, as well as a review of some of the advances made in rodent i.c.v. cannulations and whether these advances are applicable to cannulations in chickens. Using the surgical procedure described here, we were able to achieve a success rate of proper implantation of 88%, with a mortality rate of less than 1% (n = 224). • Detailed procedure for i.c.v. canula implantation in the third ventricle of 7d-old broiler chicks. • Cement cap with anchoring screws is necessary in broilers, glue does not provide sufficient stability. • Carboxylate cement and self-adhesive resin cement were tested as an alternative for methyl methacrylate cement.http://www.sciencedirect.com/science/article/pii/S2215016122001054Stereotaxic Implantation of an Intracerebroventricular Cannula
spellingShingle Chris Lamberigts
Zhigang Song
Johan Buyse
A method for intracerebroventricular cannulation of young broiler chicks
MethodsX
Stereotaxic Implantation of an Intracerebroventricular Cannula
title A method for intracerebroventricular cannulation of young broiler chicks
title_full A method for intracerebroventricular cannulation of young broiler chicks
title_fullStr A method for intracerebroventricular cannulation of young broiler chicks
title_full_unstemmed A method for intracerebroventricular cannulation of young broiler chicks
title_short A method for intracerebroventricular cannulation of young broiler chicks
title_sort method for intracerebroventricular cannulation of young broiler chicks
topic Stereotaxic Implantation of an Intracerebroventricular Cannula
url http://www.sciencedirect.com/science/article/pii/S2215016122001054
work_keys_str_mv AT chrislamberigts amethodforintracerebroventricularcannulationofyoungbroilerchicks
AT zhigangsong amethodforintracerebroventricularcannulationofyoungbroilerchicks
AT johanbuyse amethodforintracerebroventricularcannulationofyoungbroilerchicks
AT chrislamberigts methodforintracerebroventricularcannulationofyoungbroilerchicks
AT zhigangsong methodforintracerebroventricularcannulationofyoungbroilerchicks
AT johanbuyse methodforintracerebroventricularcannulationofyoungbroilerchicks