Diurnal variations in canine hematological parameters after commercial feed feeding
Complete blood count (CBC) is the evaluation of blood cells, which provides resourceful information. Considering that inadequate fasting time is the most common pre-analytical error in laboratory diagnosis and is associated with lipemia in blood samples, which has not yet been adequately investigate...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2020-08-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307 |
| _version_ | 1797944314022592512 |
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| author | Letícia Ramos Costa Nathalia Lopes Tavares da Silva Paula Lima de Oliveira Natalia Camila Minucci Bonatto Guilherme Coutinho Vieira Beatriz Perez Floriano Luiz Daniel de Barros Maria Rachel Melo Bosculo Breno Fernando Martins de Almeida |
| author_facet | Letícia Ramos Costa Nathalia Lopes Tavares da Silva Paula Lima de Oliveira Natalia Camila Minucci Bonatto Guilherme Coutinho Vieira Beatriz Perez Floriano Luiz Daniel de Barros Maria Rachel Melo Bosculo Breno Fernando Martins de Almeida |
| author_sort | Letícia Ramos Costa |
| collection | DOAJ |
| description | Complete blood count (CBC) is the evaluation of blood cells, which provides resourceful information. Considering that inadequate fasting time is the most common pre-analytical error in laboratory diagnosis and is associated with lipemia in blood samples, which has not yet been adequately investigated in dogs, this study aimed to assess diurnal postprandial changes in the CBC of healthy dogs fed with industrialized feed. Eighteen clinically healthy dogs aged 2-6 years and weighing over 6 kg were enrolled in the study. All dogs received “Premium” industrialized feed every 12 hours. Blood was collected after a 12-hour fasting period at 6:00 am (baseline), followed by feeding and new blood samples collected hourly for the next 11 consecutive hours. Red blood cell (RBC) counts, red cell distribution width (RDW), white blood cell (WBC) counts, platelets, mean platelet volume (MPV) and hemoglobin were obtained using an automated veterinary cell counter. Hematocrit (HTC) was determined by Strumia's microcapillary method, differential leukocyte count was performed on hematological dye-stained blood smears and total plasma protein (TPP) was determined using refractometry. Variables were tested for normality and differences were considered significant when p < 0.05. A statistically significant decrease was observed in the erythrogram from 2 h for RBC and hemoglobin, from 3 h for HTC and from 4 h for MCV, persisting until the end of 11 hours. There was no change in MCHC and RDW. Regarding the leukogram, a significant increase in WBC was observed from 2 to 7 h, due to the increase in segmented neutrophils 2 to 8 h following feeding. Lymphocyte counts decreased significantly at 2 and 6 h following feeding. No alteration was observed in eosinophil, basophil, monocyte and platelet counts, as well as in MPV. From 5 h to 11 h after feeding, a significant decrease was seen on TPP. However, changes in hematological parameters did not exceed reference ranges for the canine species. Feeding dogs with industrialized feed caused statistically significant changes in erythrogram, leukogram and plasma protein content. While these changes do not seem to exceed reference values for the species in healthy animals, caution is warranted for sick animals with borderline values, in which these changes might be clinically important depending on the pathologic process. |
| first_indexed | 2024-04-10T20:37:43Z |
| format | Article |
| id | doaj.art-f0edd32fd56a436898003bea72f829bc |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-10T20:37:43Z |
| publishDate | 2020-08-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-f0edd32fd56a436898003bea72f829bc2023-01-24T20:30:18ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592020-08-01415supl110.5433/1679-0359.2020v41n5supl1p2219Diurnal variations in canine hematological parameters after commercial feed feedingLetícia Ramos Costa0Nathalia Lopes Tavares da Silva1Paula Lima de Oliveira2Natalia Camila Minucci Bonatto3Guilherme Coutinho Vieira4Beatriz Perez Floriano5Luiz Daniel de Barros6Maria Rachel Melo Bosculo7Breno Fernando Martins de Almeida8Centro Universitário das Faculdades Integradas de OurinhosFaculdade de Medicina Veterinária de AraçatubaCentro Universitário das Faculdades Integradas de OurinhosUniversidade Estadual de LondrinaCentro Universitário das Faculdades Integradas de OurinhosCentro Universitário das Faculdades Integradas de OurinhosUniversidade Estadual de LondrinaCentro Universitário das Faculdades Integradas de OurinhosCentro Universitário das Faculdades Integradas de OurinhosComplete blood count (CBC) is the evaluation of blood cells, which provides resourceful information. Considering that inadequate fasting time is the most common pre-analytical error in laboratory diagnosis and is associated with lipemia in blood samples, which has not yet been adequately investigated in dogs, this study aimed to assess diurnal postprandial changes in the CBC of healthy dogs fed with industrialized feed. Eighteen clinically healthy dogs aged 2-6 years and weighing over 6 kg were enrolled in the study. All dogs received “Premium” industrialized feed every 12 hours. Blood was collected after a 12-hour fasting period at 6:00 am (baseline), followed by feeding and new blood samples collected hourly for the next 11 consecutive hours. Red blood cell (RBC) counts, red cell distribution width (RDW), white blood cell (WBC) counts, platelets, mean platelet volume (MPV) and hemoglobin were obtained using an automated veterinary cell counter. Hematocrit (HTC) was determined by Strumia's microcapillary method, differential leukocyte count was performed on hematological dye-stained blood smears and total plasma protein (TPP) was determined using refractometry. Variables were tested for normality and differences were considered significant when p < 0.05. A statistically significant decrease was observed in the erythrogram from 2 h for RBC and hemoglobin, from 3 h for HTC and from 4 h for MCV, persisting until the end of 11 hours. There was no change in MCHC and RDW. Regarding the leukogram, a significant increase in WBC was observed from 2 to 7 h, due to the increase in segmented neutrophils 2 to 8 h following feeding. Lymphocyte counts decreased significantly at 2 and 6 h following feeding. No alteration was observed in eosinophil, basophil, monocyte and platelet counts, as well as in MPV. From 5 h to 11 h after feeding, a significant decrease was seen on TPP. However, changes in hematological parameters did not exceed reference ranges for the canine species. Feeding dogs with industrialized feed caused statistically significant changes in erythrogram, leukogram and plasma protein content. While these changes do not seem to exceed reference values for the species in healthy animals, caution is warranted for sick animals with borderline values, in which these changes might be clinically important depending on the pathologic process.https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307Complete blood countDogFood. |
| spellingShingle | Letícia Ramos Costa Nathalia Lopes Tavares da Silva Paula Lima de Oliveira Natalia Camila Minucci Bonatto Guilherme Coutinho Vieira Beatriz Perez Floriano Luiz Daniel de Barros Maria Rachel Melo Bosculo Breno Fernando Martins de Almeida Diurnal variations in canine hematological parameters after commercial feed feeding Semina: Ciências Agrárias Complete blood count Dog Food. |
| title | Diurnal variations in canine hematological parameters after commercial feed feeding |
| title_full | Diurnal variations in canine hematological parameters after commercial feed feeding |
| title_fullStr | Diurnal variations in canine hematological parameters after commercial feed feeding |
| title_full_unstemmed | Diurnal variations in canine hematological parameters after commercial feed feeding |
| title_short | Diurnal variations in canine hematological parameters after commercial feed feeding |
| title_sort | diurnal variations in canine hematological parameters after commercial feed feeding |
| topic | Complete blood count Dog Food. |
| url | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307 |
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