Determination of apoptosis, proliferation status and O6-methylguanine DNA methyltransferase methylation profiles in different immunophenotypic profiles of diffuse large B-cell lymphoma
Objective: Our aim was to investigate the expression of apoptosis-associated proteins (bcl-2, bcl-xl, bax, bak, bid), apoptotic index (AI) and proliferation index (PI) in germinal center B-cell-like immunophenotypic profile (GCB) and non-GCB of diffuse large B-cell lymphoma (DLBCL). Materials and Me...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Galenos Publishing House
2011-03-01
|
| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | http://www.journalagent.com/z4/download_fulltext.asp?pdir=tjh&plng=eng&un=TJH-50480 |
| _version_ | 1797909148892921856 |
|---|---|
| author | Nilay Şen Türk Nazan Özsan Vildan Caner Nedim Karagenç Füsun Düzcan Ender Düzcan Mine Hekimgil |
| author_facet | Nilay Şen Türk Nazan Özsan Vildan Caner Nedim Karagenç Füsun Düzcan Ender Düzcan Mine Hekimgil |
| author_sort | Nilay Şen Türk |
| collection | DOAJ |
| description | Objective: Our aim was to investigate the expression of apoptosis-associated proteins (bcl-2, bcl-xl, bax, bak, bid), apoptotic index (AI) and proliferation index (PI) in germinal center B-cell-like immunophenotypic profile (GCB) and non-GCB of diffuse large B-cell lymphoma (DLBCL). Materials and Methods: The methylation status of the promoter region of O6-methylguanine-DNA yerine O6-methylguanine-DNA methyltransferase (MGMT) gene and its relation with immunophenotypic differentiation of DLBCLs were also investigated. 101 cases were classified as GCB (29 cases) or non-GCB (72 cases). Apoptosis-associated proteins and PI were determined by IHC, and TUNEL method was used to determine AI. MGMT methylation analysis was performed by real-time PCR. Results: The PI was significantly higher in GCB compared with non-GCB (p=0.011). Percentage of cells stained with bcl-6 was positively correlated with the percentage of cells expressing bcl-2 (p=0.023), AI (p=0.006) and PI (p<0.001), while a significant negative correlation was observed with the percentage of cells expressing bax (p=0.027). The percentage of cells stained with MUM1 showed a significantly positive correlation with the percentage of cells expressing bcl-xl (p=0.003), bid (p=0.002), AI (p<0.001), and PI (p=0.001). MGMT methylation analysis was performed in 95 samples, and methylated profile was found in 31 cases (32.6%). GCB was found in 6 cases (22.2%) and non-GCB was determined in 25 cases (36.8%) out of 31 with MGMT methylated samples. There was no significant association between MGMT methylation status and immunophenotypic profiles (p=0.173). Conclusion: These results suggest that bcl-6 protein expression may be responsible for the high PI in GCB. Additionally, we found that apoptosis-associated proteins were not significantly associated with immunophenotypic profiles. |
| first_indexed | 2024-04-10T11:03:49Z |
| format | Article |
| id | doaj.art-f0ede554571549b49ba39daae76e7412 |
| institution | Directory Open Access Journal |
| issn | 1300-7777 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T11:03:49Z |
| publishDate | 2011-03-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-f0ede554571549b49ba39daae76e74122023-02-15T16:19:32ZengGalenos Publishing HouseTurkish Journal of Hematology1300-77771308-52632011-03-0128011526Determination of apoptosis, proliferation status and O6-methylguanine DNA methyltransferase methylation profiles in different immunophenotypic profiles of diffuse large B-cell lymphomaNilay Şen TürkNazan ÖzsanVildan CanerNedim KaragençFüsun DüzcanEnder DüzcanMine HekimgilObjective: Our aim was to investigate the expression of apoptosis-associated proteins (bcl-2, bcl-xl, bax, bak, bid), apoptotic index (AI) and proliferation index (PI) in germinal center B-cell-like immunophenotypic profile (GCB) and non-GCB of diffuse large B-cell lymphoma (DLBCL). Materials and Methods: The methylation status of the promoter region of O6-methylguanine-DNA yerine O6-methylguanine-DNA methyltransferase (MGMT) gene and its relation with immunophenotypic differentiation of DLBCLs were also investigated. 101 cases were classified as GCB (29 cases) or non-GCB (72 cases). Apoptosis-associated proteins and PI were determined by IHC, and TUNEL method was used to determine AI. MGMT methylation analysis was performed by real-time PCR. Results: The PI was significantly higher in GCB compared with non-GCB (p=0.011). Percentage of cells stained with bcl-6 was positively correlated with the percentage of cells expressing bcl-2 (p=0.023), AI (p=0.006) and PI (p<0.001), while a significant negative correlation was observed with the percentage of cells expressing bax (p=0.027). The percentage of cells stained with MUM1 showed a significantly positive correlation with the percentage of cells expressing bcl-xl (p=0.003), bid (p=0.002), AI (p<0.001), and PI (p=0.001). MGMT methylation analysis was performed in 95 samples, and methylated profile was found in 31 cases (32.6%). GCB was found in 6 cases (22.2%) and non-GCB was determined in 25 cases (36.8%) out of 31 with MGMT methylated samples. There was no significant association between MGMT methylation status and immunophenotypic profiles (p=0.173). Conclusion: These results suggest that bcl-6 protein expression may be responsible for the high PI in GCB. Additionally, we found that apoptosis-associated proteins were not significantly associated with immunophenotypic profiles.http://www.journalagent.com/z4/download_fulltext.asp?pdir=tjh&plng=eng&un=TJH-50480LymphomaB-cellapoptosisproliferationmethylation |
| spellingShingle | Nilay Şen Türk Nazan Özsan Vildan Caner Nedim Karagenç Füsun Düzcan Ender Düzcan Mine Hekimgil Determination of apoptosis, proliferation status and O6-methylguanine DNA methyltransferase methylation profiles in different immunophenotypic profiles of diffuse large B-cell lymphoma Turkish Journal of Hematology Lymphoma B-cell apoptosis proliferation methylation |
| title | Determination of apoptosis, proliferation status and O6-methylguanine DNA methyltransferase methylation profiles in different immunophenotypic profiles of diffuse large B-cell lymphoma |
| title_full | Determination of apoptosis, proliferation status and O6-methylguanine DNA methyltransferase methylation profiles in different immunophenotypic profiles of diffuse large B-cell lymphoma |
| title_fullStr | Determination of apoptosis, proliferation status and O6-methylguanine DNA methyltransferase methylation profiles in different immunophenotypic profiles of diffuse large B-cell lymphoma |
| title_full_unstemmed | Determination of apoptosis, proliferation status and O6-methylguanine DNA methyltransferase methylation profiles in different immunophenotypic profiles of diffuse large B-cell lymphoma |
| title_short | Determination of apoptosis, proliferation status and O6-methylguanine DNA methyltransferase methylation profiles in different immunophenotypic profiles of diffuse large B-cell lymphoma |
| title_sort | determination of apoptosis proliferation status and o6 methylguanine dna methyltransferase methylation profiles in different immunophenotypic profiles of diffuse large b cell lymphoma |
| topic | Lymphoma B-cell apoptosis proliferation methylation |
| url | http://www.journalagent.com/z4/download_fulltext.asp?pdir=tjh&plng=eng&un=TJH-50480 |
| work_keys_str_mv | AT nilaysenturk determinationofapoptosisproliferationstatusando6methylguaninednamethyltransferasemethylationprofilesindifferentimmunophenotypicprofilesofdiffuselargebcelllymphoma AT nazanozsan determinationofapoptosisproliferationstatusando6methylguaninednamethyltransferasemethylationprofilesindifferentimmunophenotypicprofilesofdiffuselargebcelllymphoma AT vildancaner determinationofapoptosisproliferationstatusando6methylguaninednamethyltransferasemethylationprofilesindifferentimmunophenotypicprofilesofdiffuselargebcelllymphoma AT nedimkaragenc determinationofapoptosisproliferationstatusando6methylguaninednamethyltransferasemethylationprofilesindifferentimmunophenotypicprofilesofdiffuselargebcelllymphoma AT fusunduzcan determinationofapoptosisproliferationstatusando6methylguaninednamethyltransferasemethylationprofilesindifferentimmunophenotypicprofilesofdiffuselargebcelllymphoma AT enderduzcan determinationofapoptosisproliferationstatusando6methylguaninednamethyltransferasemethylationprofilesindifferentimmunophenotypicprofilesofdiffuselargebcelllymphoma AT minehekimgil determinationofapoptosisproliferationstatusando6methylguaninednamethyltransferasemethylationprofilesindifferentimmunophenotypicprofilesofdiffuselargebcelllymphoma |