Prognostic implications of micoRNA miR-195 expression in human tongue squamous cell carcinoma.
BACKGROUND: miR-195 is aberrantly expressed in multiple types of disease. But little is known about the dysregulation of miR-195 in tongue squamous cell carcinoma (TSCC). In this study, we investigated the roles of miR-195 in the development and progression of TSCC. METHODS: Using quantitative rever...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3579853?pdf=render |
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| author | Ling-fei Jia Su-bi Wei Kai Gong Ye-hua Gan Guang-yan Yu |
| author_facet | Ling-fei Jia Su-bi Wei Kai Gong Ye-hua Gan Guang-yan Yu |
| author_sort | Ling-fei Jia |
| collection | DOAJ |
| description | BACKGROUND: miR-195 is aberrantly expressed in multiple types of disease. But little is known about the dysregulation of miR-195 in tongue squamous cell carcinoma (TSCC). In this study, we investigated the roles of miR-195 in the development and progression of TSCC. METHODS: Using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we evaluated miR-195 expression in TSCC samples from 81 patients. Overall survival of these patients was examined using Kaplan-Meier curves with log-rank tests and the Cox proportional hazards model. The expression of two known miR-195 target genes, Cyclin D1 and Bcl-2, was also examined in the TSCC samples by immunohistochemistry. The effects of miR-195 overexpression on cell cycle progression and apoptosis and its effects on the expression of Cyclin D1 and Bcl-2 were examined in transfected TSCC cell lines (SCC-15 and Cal27) using fluorescence-activated cell sorting assays, luciferase reporter assays, and Western blots. RESULTS: Reduced miR-195 expression was associated with tumor size and the clinical stage of TSCC tumors. Kaplan-Meier survival analysis indicated that the TSCC patients with reduced expression of miR-195 had poor overall survival and in multivariable analyses low levels of miR-195 emerged as an independent prognostic factor for this clinical outcome. Levels of miR-195 expression were inversely correlated with the expression of Cyclin D1 and Bcl-2. Overexpression of miR-195 inhibited cell cycle progression, promoted apoptosis, and reduced Cyclin D1 and Bcl-2 expression in two TSCC cell lines. CONCLUSIONS: miR-195 may have potential applications as a prognostic factor for TSCC patients. |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T01:01:17Z |
| publishDate | 2013-01-01 |
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| spelling | doaj.art-f0ee6bd5412d4f81af5db88bbdd96ca02022-12-21T23:23:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5663410.1371/journal.pone.0056634Prognostic implications of micoRNA miR-195 expression in human tongue squamous cell carcinoma.Ling-fei JiaSu-bi WeiKai GongYe-hua GanGuang-yan YuBACKGROUND: miR-195 is aberrantly expressed in multiple types of disease. But little is known about the dysregulation of miR-195 in tongue squamous cell carcinoma (TSCC). In this study, we investigated the roles of miR-195 in the development and progression of TSCC. METHODS: Using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we evaluated miR-195 expression in TSCC samples from 81 patients. Overall survival of these patients was examined using Kaplan-Meier curves with log-rank tests and the Cox proportional hazards model. The expression of two known miR-195 target genes, Cyclin D1 and Bcl-2, was also examined in the TSCC samples by immunohistochemistry. The effects of miR-195 overexpression on cell cycle progression and apoptosis and its effects on the expression of Cyclin D1 and Bcl-2 were examined in transfected TSCC cell lines (SCC-15 and Cal27) using fluorescence-activated cell sorting assays, luciferase reporter assays, and Western blots. RESULTS: Reduced miR-195 expression was associated with tumor size and the clinical stage of TSCC tumors. Kaplan-Meier survival analysis indicated that the TSCC patients with reduced expression of miR-195 had poor overall survival and in multivariable analyses low levels of miR-195 emerged as an independent prognostic factor for this clinical outcome. Levels of miR-195 expression were inversely correlated with the expression of Cyclin D1 and Bcl-2. Overexpression of miR-195 inhibited cell cycle progression, promoted apoptosis, and reduced Cyclin D1 and Bcl-2 expression in two TSCC cell lines. CONCLUSIONS: miR-195 may have potential applications as a prognostic factor for TSCC patients.http://europepmc.org/articles/PMC3579853?pdf=render |
| spellingShingle | Ling-fei Jia Su-bi Wei Kai Gong Ye-hua Gan Guang-yan Yu Prognostic implications of micoRNA miR-195 expression in human tongue squamous cell carcinoma. PLoS ONE |
| title | Prognostic implications of micoRNA miR-195 expression in human tongue squamous cell carcinoma. |
| title_full | Prognostic implications of micoRNA miR-195 expression in human tongue squamous cell carcinoma. |
| title_fullStr | Prognostic implications of micoRNA miR-195 expression in human tongue squamous cell carcinoma. |
| title_full_unstemmed | Prognostic implications of micoRNA miR-195 expression in human tongue squamous cell carcinoma. |
| title_short | Prognostic implications of micoRNA miR-195 expression in human tongue squamous cell carcinoma. |
| title_sort | prognostic implications of micorna mir 195 expression in human tongue squamous cell carcinoma |
| url | http://europepmc.org/articles/PMC3579853?pdf=render |
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