Transcriptional changes of biochemical pathways in Meloidogyne incognita in response to non-fumigant nematicides
Abstract Meloidogyne incognita is a destructive and economically important agricultural pest. Similar to other plant-parasitic nematodes, management of M. incognita relies heavily on chemical controls. As old, broad spectrum, and toxic nematicides leave the market, replacements have entered includin...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14091-3 |
| _version_ | 1811242180831870976 |
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| author | Catherine L. Wram Cedar N. Hesse Inga A. Zasada |
| author_facet | Catherine L. Wram Cedar N. Hesse Inga A. Zasada |
| author_sort | Catherine L. Wram |
| collection | DOAJ |
| description | Abstract Meloidogyne incognita is a destructive and economically important agricultural pest. Similar to other plant-parasitic nematodes, management of M. incognita relies heavily on chemical controls. As old, broad spectrum, and toxic nematicides leave the market, replacements have entered including fluensulfone, fluazaindolizine, and fluopyram that are plant-parasitic nematode specific in target and less toxic to applicators. However, there is limited research into their modes-of-action and other off-target cellular effects caused by these nematicides in plant-parasitic nematodes. This study aimed to broaden the knowledge about these new nematicides by examining the transcriptional changes in M. incognita second-stage juveniles (J2) after 24-h exposure to fluensulfone, fluazaindolizine, and fluopyram as well as oxamyl, an older non-fumigant nematicide. Total RNA was extracted and sequenced using Illumina HiSeq to investigate transcriptional changes in the citric acid cycle, the glyoxylate pathway, $$\upbeta$$ β -fatty acid oxidation pathway, oxidative phosphorylation, and acetylcholine neuron components. Observed transcriptional changes in M. incognita exposed to fluopyram and oxamyl corresponded to their respective modes-of-action. Potential targets for fluensulfone and fluazaindolizine were identified in the $$\upbeta$$ β -fatty acid oxidation pathway and 2-oxoglutarate dehydrogenase of the citric acid cycle, respectively. This study provides a foundation for understanding how potential nematicide resistance could develop, identifies cellular pathways as potential nematicide targets, and determines targets for confirming unknown modes-of-action. |
| first_indexed | 2024-04-12T13:48:01Z |
| format | Article |
| id | doaj.art-f0f338b316044ea9bc7e09603c4d0d71 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T13:48:01Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f0f338b316044ea9bc7e09603c4d0d712022-12-22T03:30:37ZengNature PortfolioScientific Reports2045-23222022-06-0112111010.1038/s41598-022-14091-3Transcriptional changes of biochemical pathways in Meloidogyne incognita in response to non-fumigant nematicidesCatherine L. Wram0Cedar N. Hesse1Inga A. Zasada2Department of Botany and Plant Pathology, Oregon State UniversityUSDA-ARS Horticultural Crops Research UnitUSDA-ARS Horticultural Crops Research UnitAbstract Meloidogyne incognita is a destructive and economically important agricultural pest. Similar to other plant-parasitic nematodes, management of M. incognita relies heavily on chemical controls. As old, broad spectrum, and toxic nematicides leave the market, replacements have entered including fluensulfone, fluazaindolizine, and fluopyram that are plant-parasitic nematode specific in target and less toxic to applicators. However, there is limited research into their modes-of-action and other off-target cellular effects caused by these nematicides in plant-parasitic nematodes. This study aimed to broaden the knowledge about these new nematicides by examining the transcriptional changes in M. incognita second-stage juveniles (J2) after 24-h exposure to fluensulfone, fluazaindolizine, and fluopyram as well as oxamyl, an older non-fumigant nematicide. Total RNA was extracted and sequenced using Illumina HiSeq to investigate transcriptional changes in the citric acid cycle, the glyoxylate pathway, $$\upbeta$$ β -fatty acid oxidation pathway, oxidative phosphorylation, and acetylcholine neuron components. Observed transcriptional changes in M. incognita exposed to fluopyram and oxamyl corresponded to their respective modes-of-action. Potential targets for fluensulfone and fluazaindolizine were identified in the $$\upbeta$$ β -fatty acid oxidation pathway and 2-oxoglutarate dehydrogenase of the citric acid cycle, respectively. This study provides a foundation for understanding how potential nematicide resistance could develop, identifies cellular pathways as potential nematicide targets, and determines targets for confirming unknown modes-of-action.https://doi.org/10.1038/s41598-022-14091-3 |
| spellingShingle | Catherine L. Wram Cedar N. Hesse Inga A. Zasada Transcriptional changes of biochemical pathways in Meloidogyne incognita in response to non-fumigant nematicides Scientific Reports |
| title | Transcriptional changes of biochemical pathways in Meloidogyne incognita in response to non-fumigant nematicides |
| title_full | Transcriptional changes of biochemical pathways in Meloidogyne incognita in response to non-fumigant nematicides |
| title_fullStr | Transcriptional changes of biochemical pathways in Meloidogyne incognita in response to non-fumigant nematicides |
| title_full_unstemmed | Transcriptional changes of biochemical pathways in Meloidogyne incognita in response to non-fumigant nematicides |
| title_short | Transcriptional changes of biochemical pathways in Meloidogyne incognita in response to non-fumigant nematicides |
| title_sort | transcriptional changes of biochemical pathways in meloidogyne incognita in response to non fumigant nematicides |
| url | https://doi.org/10.1038/s41598-022-14091-3 |
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