Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) have been successfully isolated from a broad range of adult, fetal, and other nonembryonic tissues. Fetal lung has been identified as a rich source of MSCs. However, the biological characteristics and differentiation potential of fetal lung MSCs remain to be explored. I...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2005-05-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000005783983070 |
| _version_ | 1818402278124552192 |
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| author | Cun Gang Fan Feng Wu Tang Qing Jun Zhang Shi Hong Lu Hai Ying Liu Zong Mao Zhao Bin Liu Zhi Bo Han Zhong Chao Han |
| author_facet | Cun Gang Fan Feng Wu Tang Qing Jun Zhang Shi Hong Lu Hai Ying Liu Zong Mao Zhao Bin Liu Zhi Bo Han Zhong Chao Han |
| author_sort | Cun Gang Fan |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) have been successfully isolated from a broad range of adult, fetal, and other nonembryonic tissues. Fetal lung has been identified as a rich source of MSCs. However, the biological characteristics and differentiation potential of fetal lung MSCs remain to be explored. In this study, we established a series of methods for isolation and expansion of fetal lung MSCs. These MSCs could withstand more than 40 passages without obvious decline in proliferation ability, significant changes in morphology, and expression of cell markers. Flow cytometric analysis showed that fetal lung MSCs expressed CD13, CD29, CD44, CD90, CD105, CD166, and HLA-ABC, but not CD14, CD31, CD34, CD38, CD41a, CD42b, CD45, CD49d, CD61, CD106, CD133, and HLA-DR. Cell cycle analysis revealed that when the MSCs reached their log phase of growth, more than 90% of the cells were in G 0 /G 1 phase while the proportion of cells in S phase and G 2 /M phase were about 5.56% and 2.08% cells, respectively. These MSCs could differentiate into neural cells in addition to their mesenchymal differentiation potential. Our data suggest that the fetal lung MSC population is an alternative source of stem cells for cell-based therapy of neurological defects or mesenchymal-originating diseases. |
| first_indexed | 2024-12-14T08:05:49Z |
| format | Article |
| id | doaj.art-f0f5a0c8856945a2a33819db19728cfe |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-14T08:05:49Z |
| publishDate | 2005-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-f0f5a0c8856945a2a33819db19728cfe2022-12-21T23:10:12ZengSAGE PublishingCell Transplantation0963-68971555-38922005-05-011410.3727/000000005783983070Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem CellsCun Gang Fan0Feng Wu Tang1Qing Jun Zhang2Shi Hong Lu3Hai Ying Liu4Zong Mao Zhao5Bin Liu6Zhi Bo Han7Zhong Chao Han8Department of Neurosurgery, the Second Hospital of Hebei Medical University, Shijiazhuang, People's Republic of ChinaNational Research Center for Stem Cell Engineering & Technology, State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People's Republic of ChinaDepartment of Neurosurgery, the Second Hospital of Hebei Medical University, Shijiazhuang, People's Republic of ChinaNational Research Center for Stem Cell Engineering & Technology, State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People's Republic of ChinaDepartment of Neurosurgery, the Second Hospital of Hebei Medical University, Shijiazhuang, People's Republic of ChinaDepartment of Neurosurgery, the Second Hospital of Hebei Medical University, Shijiazhuang, People's Republic of ChinaNational Research Center for Stem Cell Engineering & Technology, State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People's Republic of ChinaNational Research Center for Stem Cell Engineering & Technology, State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People's Republic of ChinaNational Research Center for Stem Cell Engineering & Technology, State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People's Republic of ChinaMesenchymal stem cells (MSCs) have been successfully isolated from a broad range of adult, fetal, and other nonembryonic tissues. Fetal lung has been identified as a rich source of MSCs. However, the biological characteristics and differentiation potential of fetal lung MSCs remain to be explored. In this study, we established a series of methods for isolation and expansion of fetal lung MSCs. These MSCs could withstand more than 40 passages without obvious decline in proliferation ability, significant changes in morphology, and expression of cell markers. Flow cytometric analysis showed that fetal lung MSCs expressed CD13, CD29, CD44, CD90, CD105, CD166, and HLA-ABC, but not CD14, CD31, CD34, CD38, CD41a, CD42b, CD45, CD49d, CD61, CD106, CD133, and HLA-DR. Cell cycle analysis revealed that when the MSCs reached their log phase of growth, more than 90% of the cells were in G 0 /G 1 phase while the proportion of cells in S phase and G 2 /M phase were about 5.56% and 2.08% cells, respectively. These MSCs could differentiate into neural cells in addition to their mesenchymal differentiation potential. Our data suggest that the fetal lung MSC population is an alternative source of stem cells for cell-based therapy of neurological defects or mesenchymal-originating diseases.https://doi.org/10.3727/000000005783983070 |
| spellingShingle | Cun Gang Fan Feng Wu Tang Qing Jun Zhang Shi Hong Lu Hai Ying Liu Zong Mao Zhao Bin Liu Zhi Bo Han Zhong Chao Han Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem Cells Cell Transplantation |
| title | Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem Cells |
| title_full | Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem Cells |
| title_fullStr | Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem Cells |
| title_full_unstemmed | Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem Cells |
| title_short | Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem Cells |
| title_sort | characterization and neural differentiation of fetal lung mesenchymal stem cells |
| url | https://doi.org/10.3727/000000005783983070 |
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