Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro Study
Endo-periodontal lesions are challenging clinical situations where both the supporting tissues and the root canal of the same tooth are infected. In the present study, chlorhexidine (CHX)-loaded calcium hydroxide (CH) pastes were used as intracanal medications (ICMs). They were prepared and tested o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/9/1416 |
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| author | Kadiatou Sy Charlène Chevalier Mickaël Maton Ilham Mokbel Séverine Mahieux Isabelle Houcke Christel Neut Brigitte Grosgogeat Etienne Deveaux Kerstin Gritsch Kevimy Agossa |
| author_facet | Kadiatou Sy Charlène Chevalier Mickaël Maton Ilham Mokbel Séverine Mahieux Isabelle Houcke Christel Neut Brigitte Grosgogeat Etienne Deveaux Kerstin Gritsch Kevimy Agossa |
| author_sort | Kadiatou Sy |
| collection | DOAJ |
| description | Endo-periodontal lesions are challenging clinical situations where both the supporting tissues and the root canal of the same tooth are infected. In the present study, chlorhexidine (CHX)-loaded calcium hydroxide (CH) pastes were used as intracanal medications (ICMs). They were prepared and tested on pathogens found in both the root canal and the periodontal pocket. Exposure to 0.5% and 1% CHX-loaded ICMs decreased the growth of <i>Porphyromonas gingivalis</i> and was effective in eradicating or inhibiting an <i>Enterococcus faecalis</i> biofilm. CH was injected into the root canal of extracted human teeth immersed in deionized water. CHX-loaded ICMs resulted in the transradicular diffusion of active components outside the tooth through the apex and the lateral dentinal tubules, as shown by the release of CHX (from 3.99 µg/mL to 51.28 µg/mL) and changes in pH (from 6.63 to 8.18) and calcium concentrations (from 2.42 ppm to 14.67 ppm) after 7 days. The 0.5% CHX-loaded ICM was non-toxic and reduced the release of IL-6 by periodontal cells stimulated by <i>P. gingivalis</i> lipopolysaccharides. Results indicate that the root canal may serve as a reservoir for periodontal drug delivery and that CHX-based ICMs can be an adjuvant for the control of infections and inflammation in endo-periodontal lesions. |
| first_indexed | 2024-03-10T23:06:57Z |
| format | Article |
| id | doaj.art-f0fc7fce504e4502ae8447a4dc057ac1 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T23:06:57Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-f0fc7fce504e4502ae8447a4dc057ac12023-11-19T09:17:28ZengMDPI AGAntibiotics2079-63822023-09-01129141610.3390/antibiotics12091416Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro StudyKadiatou Sy0Charlène Chevalier1Mickaël Maton2Ilham Mokbel3Séverine Mahieux4Isabelle Houcke5Christel Neut6Brigitte Grosgogeat7Etienne Deveaux8Kerstin Gritsch9Kevimy Agossa10U1008, Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, Université de Lille, 59000 Lille, FranceUMR CNRS 5615 Laboratoire des Multimatériaux et Interfaces, Université Lyon 1, 69100 Villeurbanne, FranceU1008, Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, Université de Lille, 59000 Lille, FranceUMR CNRS 5615 Laboratoire des Multimatériaux et Interfaces, Université Lyon 1, 69100 Villeurbanne, FranceU1286 Infinite, Institute for Translational Research in Inflammation, Inserm, CHU Lille, Université de Lille, 59000 Lille, FranceU1286 Infinite, Institute for Translational Research in Inflammation, Inserm, CHU Lille, Université de Lille, 59000 Lille, FranceU1286 Infinite, Institute for Translational Research in Inflammation, Inserm, CHU Lille, Université de Lille, 59000 Lille, FranceFaculté d’Odontologie, Hospices Civils de Lyon, Pôle d′Odontologie, Université Lyon 1, Université de Lyon, 69372 Lyon Cedex 08, FranceU1008, Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, Université de Lille, 59000 Lille, FranceFaculté d’Odontologie, Hospices Civils de Lyon, Pôle d′Odontologie, Université Lyon 1, Université de Lyon, 69372 Lyon Cedex 08, FranceU1008, Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, Université de Lille, 59000 Lille, FranceEndo-periodontal lesions are challenging clinical situations where both the supporting tissues and the root canal of the same tooth are infected. In the present study, chlorhexidine (CHX)-loaded calcium hydroxide (CH) pastes were used as intracanal medications (ICMs). They were prepared and tested on pathogens found in both the root canal and the periodontal pocket. Exposure to 0.5% and 1% CHX-loaded ICMs decreased the growth of <i>Porphyromonas gingivalis</i> and was effective in eradicating or inhibiting an <i>Enterococcus faecalis</i> biofilm. CH was injected into the root canal of extracted human teeth immersed in deionized water. CHX-loaded ICMs resulted in the transradicular diffusion of active components outside the tooth through the apex and the lateral dentinal tubules, as shown by the release of CHX (from 3.99 µg/mL to 51.28 µg/mL) and changes in pH (from 6.63 to 8.18) and calcium concentrations (from 2.42 ppm to 14.67 ppm) after 7 days. The 0.5% CHX-loaded ICM was non-toxic and reduced the release of IL-6 by periodontal cells stimulated by <i>P. gingivalis</i> lipopolysaccharides. Results indicate that the root canal may serve as a reservoir for periodontal drug delivery and that CHX-based ICMs can be an adjuvant for the control of infections and inflammation in endo-periodontal lesions.https://www.mdpi.com/2079-6382/12/9/1416endo-periodontal lesionsintracanal medicationion releaselocal drug deliveryperiodontal cells |
| spellingShingle | Kadiatou Sy Charlène Chevalier Mickaël Maton Ilham Mokbel Séverine Mahieux Isabelle Houcke Christel Neut Brigitte Grosgogeat Etienne Deveaux Kerstin Gritsch Kevimy Agossa Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro Study Antibiotics endo-periodontal lesions intracanal medication ion release local drug delivery periodontal cells |
| title | Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro Study |
| title_full | Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro Study |
| title_fullStr | Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro Study |
| title_full_unstemmed | Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro Study |
| title_short | Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro Study |
| title_sort | therapeutic potential of chlorhexidine loaded calcium hydroxide based intracanal medications in endo periodontal lesions an ex vivo and in vitro study |
| topic | endo-periodontal lesions intracanal medication ion release local drug delivery periodontal cells |
| url | https://www.mdpi.com/2079-6382/12/9/1416 |
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