Assessment of Ultrasonic Stress on Survival and β-Glucosidase Activity of Encapsulated <i>Lactiplantibacillus plantarum</i> BCRC 10357 in Fermentation of Black Soymilk
The enhanced β-glucosidase activity of encapsulated <i>Lactiplantibacillus plantarum</i> BCRC 10357 within calcium alginate capsules was investigated by ultrasonic stimulation to induce the stress response of the bacteria for the biotransformation of isoflavones in black soymilk. The eff...
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MDPI AG
2022-04-01
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| Online Access: | https://www.mdpi.com/2304-8158/11/9/1234 |
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| author | Hung-Chih Tseng Chun-Yao Yang |
| author_facet | Hung-Chih Tseng Chun-Yao Yang |
| author_sort | Hung-Chih Tseng |
| collection | DOAJ |
| description | The enhanced β-glucosidase activity of encapsulated <i>Lactiplantibacillus plantarum</i> BCRC 10357 within calcium alginate capsules was investigated by ultrasonic stimulation to induce the stress response of the bacteria for the biotransformation of isoflavones in black soymilk. The effects of various ultrasound durations, sodium alginate concentrations (% ALG), and cell suspensions on the β-glucosidase activity of encapsulated bacteria were explored. The β-glucosidase activity of encapsulated <i>L. plantarum</i> BCRC 10357 with ultrasonic stimulation (40 kHz/300 W) was greater than that without ultrasound. With 20 min of ultrasonic treatment, the β-glucosidase activity of encapsulated <i>L. plantarum</i> BCRC 10357 from 2% ALG/0.85% NaCl cell suspension was 11.47 U/mL at 12 h, then increased to 27.43 U/mL at 36 h and to 26.25 U/mL at 48 h in black soymilk at 37 °C, showing the high adaptation of encapsulated <i>L. plantarum</i> BCRC 10357 encountering ultrasonic stress to release high β-glucosidase until 48 h, at which point the ratio of isoflavone aglycones (daidzein and genistein) in total isoflavones (daidzin, genistin, daidzein, and genistein) was 98.65%, reflecting the effective biotransformation of isoflavone glycosides into aglycones by β-glucosidase. In this study, the survivability and β-glucosidase activity of encapsulated <i>L. plantarum</i> BCRC 10357 were enhanced under ultrasonic stimulation, and were favorably used in the fermentation of black soymilk. |
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| spelling | doaj.art-f0fcb2e79cb345f9ab58346afd8386792023-11-23T08:12:37ZengMDPI AGFoods2304-81582022-04-01119123410.3390/foods11091234Assessment of Ultrasonic Stress on Survival and β-Glucosidase Activity of Encapsulated <i>Lactiplantibacillus plantarum</i> BCRC 10357 in Fermentation of Black SoymilkHung-Chih Tseng0Chun-Yao Yang1Department of Food Science, Fu Jen Catholic University, No. 510, Zhongzheng Rd., Xinzhuang District, New Taipei City 242062, TaiwanDepartment of Food Science, Fu Jen Catholic University, No. 510, Zhongzheng Rd., Xinzhuang District, New Taipei City 242062, TaiwanThe enhanced β-glucosidase activity of encapsulated <i>Lactiplantibacillus plantarum</i> BCRC 10357 within calcium alginate capsules was investigated by ultrasonic stimulation to induce the stress response of the bacteria for the biotransformation of isoflavones in black soymilk. The effects of various ultrasound durations, sodium alginate concentrations (% ALG), and cell suspensions on the β-glucosidase activity of encapsulated bacteria were explored. The β-glucosidase activity of encapsulated <i>L. plantarum</i> BCRC 10357 with ultrasonic stimulation (40 kHz/300 W) was greater than that without ultrasound. With 20 min of ultrasonic treatment, the β-glucosidase activity of encapsulated <i>L. plantarum</i> BCRC 10357 from 2% ALG/0.85% NaCl cell suspension was 11.47 U/mL at 12 h, then increased to 27.43 U/mL at 36 h and to 26.25 U/mL at 48 h in black soymilk at 37 °C, showing the high adaptation of encapsulated <i>L. plantarum</i> BCRC 10357 encountering ultrasonic stress to release high β-glucosidase until 48 h, at which point the ratio of isoflavone aglycones (daidzein and genistein) in total isoflavones (daidzin, genistin, daidzein, and genistein) was 98.65%, reflecting the effective biotransformation of isoflavone glycosides into aglycones by β-glucosidase. In this study, the survivability and β-glucosidase activity of encapsulated <i>L. plantarum</i> BCRC 10357 were enhanced under ultrasonic stimulation, and were favorably used in the fermentation of black soymilk.https://www.mdpi.com/2304-8158/11/9/1234stress responseultrasonic stimulation<i>Lactiplantibacillus plantarum</i>encapsulationfermentation |
| spellingShingle | Hung-Chih Tseng Chun-Yao Yang Assessment of Ultrasonic Stress on Survival and β-Glucosidase Activity of Encapsulated <i>Lactiplantibacillus plantarum</i> BCRC 10357 in Fermentation of Black Soymilk Foods stress response ultrasonic stimulation <i>Lactiplantibacillus plantarum</i> encapsulation fermentation |
| title | Assessment of Ultrasonic Stress on Survival and β-Glucosidase Activity of Encapsulated <i>Lactiplantibacillus plantarum</i> BCRC 10357 in Fermentation of Black Soymilk |
| title_full | Assessment of Ultrasonic Stress on Survival and β-Glucosidase Activity of Encapsulated <i>Lactiplantibacillus plantarum</i> BCRC 10357 in Fermentation of Black Soymilk |
| title_fullStr | Assessment of Ultrasonic Stress on Survival and β-Glucosidase Activity of Encapsulated <i>Lactiplantibacillus plantarum</i> BCRC 10357 in Fermentation of Black Soymilk |
| title_full_unstemmed | Assessment of Ultrasonic Stress on Survival and β-Glucosidase Activity of Encapsulated <i>Lactiplantibacillus plantarum</i> BCRC 10357 in Fermentation of Black Soymilk |
| title_short | Assessment of Ultrasonic Stress on Survival and β-Glucosidase Activity of Encapsulated <i>Lactiplantibacillus plantarum</i> BCRC 10357 in Fermentation of Black Soymilk |
| title_sort | assessment of ultrasonic stress on survival and β glucosidase activity of encapsulated i lactiplantibacillus plantarum i bcrc 10357 in fermentation of black soymilk |
| topic | stress response ultrasonic stimulation <i>Lactiplantibacillus plantarum</i> encapsulation fermentation |
| url | https://www.mdpi.com/2304-8158/11/9/1234 |
| work_keys_str_mv | AT hungchihtseng assessmentofultrasonicstressonsurvivalandbglucosidaseactivityofencapsulatedilactiplantibacillusplantarumibcrc10357infermentationofblacksoymilk AT chunyaoyang assessmentofultrasonicstressonsurvivalandbglucosidaseactivityofencapsulatedilactiplantibacillusplantarumibcrc10357infermentationofblacksoymilk |