The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition
Abstract Background Understanding the composition of the microbial community and its functional capacity during weaning is important for pig production as bacteria play important roles in the pig’s health and growth performance. However, limited information is available regarding the composition and...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-07-01
|
| Series: | Journal of Animal Science and Biotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40104-018-0269-6 |
| _version_ | 1818614981652905984 |
|---|---|
| author | Robin B. Guevarra Sang Hyun Hong Jin Ho Cho Bo-Ra Kim Jiwon Shin Jun Hyung Lee Bit Na Kang Young Hwa Kim Suphot Wattanaphansak Richard E. Isaacson Minho Song Hyeun Bum Kim |
| author_facet | Robin B. Guevarra Sang Hyun Hong Jin Ho Cho Bo-Ra Kim Jiwon Shin Jun Hyung Lee Bit Na Kang Young Hwa Kim Suphot Wattanaphansak Richard E. Isaacson Minho Song Hyeun Bum Kim |
| author_sort | Robin B. Guevarra |
| collection | DOAJ |
| description | Abstract Background Understanding the composition of the microbial community and its functional capacity during weaning is important for pig production as bacteria play important roles in the pig’s health and growth performance. However, limited information is available regarding the composition and function of the gut microbiome of piglets in early-life. Therefore, we performed 16S rRNA gene and whole metagenome shotgun sequencing of DNA from fecal samples from healthy piglets during weaning to measure microbiome shifts, and to identify the potential contribution of the early-life microbiota in shaping piglet health with a focus on microbial stress responses, carbohydrate and amino acid metabolism. Results The analysis of 16S rRNA genes and whole metagenome shotgun sequencing revealed significant compositional and functional differences between the fecal microbiome in nursing and weaned piglets. The fecal microbiome of the nursing piglets showed higher relative abundance of bacteria in the genus Bacteroides with abundant gene families related to the utilization of lactose and galactose. Prevotella and Lactobacillus were enriched in weaned piglets with an enrichment for the gene families associated with carbohydrate and amino acid metabolism. In addition, an analysis of the functional capacity of the fecal microbiome showed higher abundances of genes associated with heat shock and oxidative stress in the metagenome of weaned piglets compared to nursing piglets. Conclusions Overall, our data show that microbial shifts and changes in functional capacities of the piglet fecal microbiome resulted in potential reductions in the effects of stress, including dietary changes that occur during weaning. These results provide us with new insights into the piglet gut microbiome that contributes to the growth of the animal. |
| first_indexed | 2024-12-16T16:26:39Z |
| format | Article |
| id | doaj.art-f0fea1a6c23243319a97d4f5f2d72245 |
| institution | Directory Open Access Journal |
| issn | 2049-1891 |
| language | English |
| last_indexed | 2024-12-16T16:26:39Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Animal Science and Biotechnology |
| spelling | doaj.art-f0fea1a6c23243319a97d4f5f2d722452022-12-21T22:24:45ZengBMCJournal of Animal Science and Biotechnology2049-18912018-07-01911910.1186/s40104-018-0269-6The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutritionRobin B. Guevarra0Sang Hyun Hong1Jin Ho Cho2Bo-Ra Kim3Jiwon Shin4Jun Hyung Lee5Bit Na Kang6Young Hwa Kim7Suphot Wattanaphansak8Richard E. Isaacson9Minho Song10Hyeun Bum Kim11Department of Animal Resources Science, Dankook UniversityDepartment of Animal Resources Science, Dankook UniversityDivision of Food and Animal Sciences, Chungbuk National UniversityDepartment of Animal Resources Science, Dankook UniversityDepartment of Animal Resources Science, Dankook UniversityDepartment of Animal Resources Science, Dankook UniversityAbbvie Bioresearch CenterNational Institute of Animal Science, Rural Development AdministrationDepartment of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary and Biomedical Sciences, University of MinnesotaDivision of Animal and Dairy Science, Chungnam National UniversityDepartment of Animal Resources Science, Dankook UniversityAbstract Background Understanding the composition of the microbial community and its functional capacity during weaning is important for pig production as bacteria play important roles in the pig’s health and growth performance. However, limited information is available regarding the composition and function of the gut microbiome of piglets in early-life. Therefore, we performed 16S rRNA gene and whole metagenome shotgun sequencing of DNA from fecal samples from healthy piglets during weaning to measure microbiome shifts, and to identify the potential contribution of the early-life microbiota in shaping piglet health with a focus on microbial stress responses, carbohydrate and amino acid metabolism. Results The analysis of 16S rRNA genes and whole metagenome shotgun sequencing revealed significant compositional and functional differences between the fecal microbiome in nursing and weaned piglets. The fecal microbiome of the nursing piglets showed higher relative abundance of bacteria in the genus Bacteroides with abundant gene families related to the utilization of lactose and galactose. Prevotella and Lactobacillus were enriched in weaned piglets with an enrichment for the gene families associated with carbohydrate and amino acid metabolism. In addition, an analysis of the functional capacity of the fecal microbiome showed higher abundances of genes associated with heat shock and oxidative stress in the metagenome of weaned piglets compared to nursing piglets. Conclusions Overall, our data show that microbial shifts and changes in functional capacities of the piglet fecal microbiome resulted in potential reductions in the effects of stress, including dietary changes that occur during weaning. These results provide us with new insights into the piglet gut microbiome that contributes to the growth of the animal.http://link.springer.com/article/10.1186/s40104-018-0269-6MetagenomicsMicrobiomePiglets16S rRNAWeaning |
| spellingShingle | Robin B. Guevarra Sang Hyun Hong Jin Ho Cho Bo-Ra Kim Jiwon Shin Jun Hyung Lee Bit Na Kang Young Hwa Kim Suphot Wattanaphansak Richard E. Isaacson Minho Song Hyeun Bum Kim The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition Journal of Animal Science and Biotechnology Metagenomics Microbiome Piglets 16S rRNA Weaning |
| title | The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition |
| title_full | The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition |
| title_fullStr | The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition |
| title_full_unstemmed | The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition |
| title_short | The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition |
| title_sort | dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition |
| topic | Metagenomics Microbiome Piglets 16S rRNA Weaning |
| url | http://link.springer.com/article/10.1186/s40104-018-0269-6 |
| work_keys_str_mv | AT robinbguevarra thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT sanghyunhong thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT jinhocho thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT borakim thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT jiwonshin thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT junhyunglee thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT bitnakang thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT younghwakim thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT suphotwattanaphansak thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT richardeisaacson thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT minhosong thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT hyeunbumkim thedynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT robinbguevarra dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT sanghyunhong dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT jinhocho dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT borakim dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT jiwonshin dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT junhyunglee dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT bitnakang dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT younghwakim dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT suphotwattanaphansak dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT richardeisaacson dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT minhosong dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition AT hyeunbumkim dynamicsofthepigletgutmicrobiomeduringtheweaningtransitioninassociationwithhealthandnutrition |