Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts
Polymeric membranes are employed in guided bone regeneration (GBR) as physical barriers to facilitate bone in-growth. A bioactive and biomimetic membrane with the ability to participate in the healing and regeneration of the bone is necessary. The aim of the present study was to analyze how novel si...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/7/1063 |
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| author | Manuel Toledano-Osorio Francisco J. Manzano-Moreno Manuel Toledano Antonio L. Medina-Castillo Victor J. Costela-Ruiz Concepción Ruiz Raquel Osorio |
| author_facet | Manuel Toledano-Osorio Francisco J. Manzano-Moreno Manuel Toledano Antonio L. Medina-Castillo Victor J. Costela-Ruiz Concepción Ruiz Raquel Osorio |
| author_sort | Manuel Toledano-Osorio |
| collection | DOAJ |
| description | Polymeric membranes are employed in guided bone regeneration (GBR) as physical barriers to facilitate bone in-growth. A bioactive and biomimetic membrane with the ability to participate in the healing and regeneration of the bone is necessary. The aim of the present study was to analyze how novel silicon dioxide composite membranes functionalized with zinc or doxycycline can modulate the osteoblasts’ proliferation, differentiation, and expression of selected antigenic markers related to immunomodulation. Nanostructured acrylate-based membranes were developed, blended with silica, and functionalized with zinc or doxycycline. They were subjected to MG63 osteoblast-like cells culturing. Proliferation was assessed by MTT-assay, differentiation by evaluating the alkaline phosphatase activity by a spectrophotometric method and antigenic phenotype was assessed by flow cytometry for selected markers. Mean comparisons were conducted by one-way ANOVA and Tukey tests (<i>p</i> < 0.05). The blending of silica nanoparticles in the tested non-resorbable polymeric scaffold improved the proliferation and differentiation of osteoblasts, but doxycycline doped scaffolds attained the best results. Osteoblasts cultured on doxycycline functionalized membranes presented higher expression of CD54, CD80, CD86, and HLA-DR, indicating a beneficial immunomodulation activity. Doxycycline doped membranes may be a potential candidate for use in GBR procedures in several challenging pathologies, including periodontal disease. |
| first_indexed | 2024-03-10T12:50:50Z |
| format | Article |
| id | doaj.art-f0feffde536146188c3a43b456b842f5 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T12:50:50Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-f0feffde536146188c3a43b456b842f52023-11-21T13:08:06ZengMDPI AGPolymers2073-43602021-03-01137106310.3390/polym13071063Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of OsteoblastsManuel Toledano-Osorio0Francisco J. Manzano-Moreno1Manuel Toledano2Antonio L. Medina-Castillo3Victor J. Costela-Ruiz4Concepción Ruiz5Raquel Osorio6Colegio Máximo de Cartuja s/n, Faculty of Dentistry, University of Granada, 18071 Granada, SpainBiomedical Group (BIO277), Department of Stomatology, School of Dentistry, University of Granada, 18071 Granada, SpainColegio Máximo de Cartuja s/n, Faculty of Dentistry, University of Granada, 18071 Granada, SpainNanoMyP<sup>®</sup>, Nanomateriales y Polimeros S.L., Spin-Off Company of the University of Granada, Edificio BIC-Granada, Av. Innovación 1, Armilla, 18016 Granada, SpainInstituto Investigación Biosanitaria, ibs.Granada, 18071 Granada, SpainInstituto Investigación Biosanitaria, ibs.Granada, 18071 Granada, SpainColegio Máximo de Cartuja s/n, Faculty of Dentistry, University of Granada, 18071 Granada, SpainPolymeric membranes are employed in guided bone regeneration (GBR) as physical barriers to facilitate bone in-growth. A bioactive and biomimetic membrane with the ability to participate in the healing and regeneration of the bone is necessary. The aim of the present study was to analyze how novel silicon dioxide composite membranes functionalized with zinc or doxycycline can modulate the osteoblasts’ proliferation, differentiation, and expression of selected antigenic markers related to immunomodulation. Nanostructured acrylate-based membranes were developed, blended with silica, and functionalized with zinc or doxycycline. They were subjected to MG63 osteoblast-like cells culturing. Proliferation was assessed by MTT-assay, differentiation by evaluating the alkaline phosphatase activity by a spectrophotometric method and antigenic phenotype was assessed by flow cytometry for selected markers. Mean comparisons were conducted by one-way ANOVA and Tukey tests (<i>p</i> < 0.05). The blending of silica nanoparticles in the tested non-resorbable polymeric scaffold improved the proliferation and differentiation of osteoblasts, but doxycycline doped scaffolds attained the best results. Osteoblasts cultured on doxycycline functionalized membranes presented higher expression of CD54, CD80, CD86, and HLA-DR, indicating a beneficial immunomodulation activity. Doxycycline doped membranes may be a potential candidate for use in GBR procedures in several challenging pathologies, including periodontal disease.https://www.mdpi.com/2073-4360/13/7/1063CD markersdoxycyclineantigenic phenotypeosteoblastsmembrane |
| spellingShingle | Manuel Toledano-Osorio Francisco J. Manzano-Moreno Manuel Toledano Antonio L. Medina-Castillo Victor J. Costela-Ruiz Concepción Ruiz Raquel Osorio Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts Polymers CD markers doxycycline antigenic phenotype osteoblasts membrane |
| title | Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts |
| title_full | Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts |
| title_fullStr | Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts |
| title_full_unstemmed | Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts |
| title_short | Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts |
| title_sort | doxycycline doped polymeric membranes induced growth differentiation and expression of antigenic phenotype markers of osteoblasts |
| topic | CD markers doxycycline antigenic phenotype osteoblasts membrane |
| url | https://www.mdpi.com/2073-4360/13/7/1063 |
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