Distinct rhizosphere soil responses to nitrogen in relation to microbial biomass and community composition at initial flowering stages of alfalfa cultivars
In the long-term growth process, alfalfa rhizosphere forms specific microbiome to provide nutrition for its growth and development. However, the effects of different perennial alfalfa cultivars on changes in the rhizosphere soil characteristics and microbiome are not well understood. In this study,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.938865/full |
| _version_ | 1817997147714355200 |
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| author | Yunru An Haoyang Sun Wei Zhang Yunfu Sun Shuxia Li Zhouchang Yu Rongchen Yang Tianming Hu Peizhi Yang |
| author_facet | Yunru An Haoyang Sun Wei Zhang Yunfu Sun Shuxia Li Zhouchang Yu Rongchen Yang Tianming Hu Peizhi Yang |
| author_sort | Yunru An |
| collection | DOAJ |
| description | In the long-term growth process, alfalfa rhizosphere forms specific microbiome to provide nutrition for its growth and development. However, the effects of different perennial alfalfa cultivars on changes in the rhizosphere soil characteristics and microbiome are not well understood. In this study, 12 perennial alfalfa cultivars were grown continuously for eight years. Rhizosphere samples were tested using Illumina sequencing of the 16S rRNA gene coupled with co-occurrence network analysis to explore the relationship between alfalfa (biomass and crude protein content), soil properties, and the microbial composition and diversity. Redundancy analysis showed SOC and pH had the greatest impact on the composition of the rhizosphere microbial community. Moreover, microbial diversity also contributes to microbial composition. Soil properties (AP, EC, SOC and pH) exhibited a significant positive correlation with soil bacterial communities, which was attributed to the differences between plant cultivars. Partial least squares path modeling (PLS-PM) revealed that microbial biomass and community composition rather than diversity, are the dominant determinants in the rhizosphere soil nitrogen content of perennial alfalfa. Our findings demonstrate that the soil microbial biomass and composition of rhizosphere bacterial communities are strongly affected by cultivar, driving the changes in soil nitrogen content, and variances in the selective capacities of plants. |
| first_indexed | 2024-04-14T02:32:47Z |
| format | Article |
| id | doaj.art-f0ff01827d014f779b41d756c5f55694 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-14T02:32:47Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f0ff01827d014f779b41d756c5f556942022-12-22T02:17:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-08-011310.3389/fpls.2022.938865938865Distinct rhizosphere soil responses to nitrogen in relation to microbial biomass and community composition at initial flowering stages of alfalfa cultivarsYunru An0Haoyang Sun1Wei Zhang2Yunfu Sun3Shuxia Li4Zhouchang Yu5Rongchen Yang6Tianming Hu7Peizhi Yang8College of Grassland Agriculture, Northwest A&F University, Xianyang, ChinaCollege of Grassland Agriculture, Northwest A&F University, Xianyang, ChinaCollege of Grassland Agriculture, Northwest A&F University, Xianyang, ChinaCollege of Grassland Agriculture, Northwest A&F University, Xianyang, ChinaCollege of Agricultural, Ningxia University, Yinchuan, ChinaCollege of Grassland Agriculture, Northwest A&F University, Xianyang, ChinaCollege of Grassland Agriculture, Northwest A&F University, Xianyang, ChinaCollege of Grassland Agriculture, Northwest A&F University, Xianyang, ChinaCollege of Grassland Agriculture, Northwest A&F University, Xianyang, ChinaIn the long-term growth process, alfalfa rhizosphere forms specific microbiome to provide nutrition for its growth and development. However, the effects of different perennial alfalfa cultivars on changes in the rhizosphere soil characteristics and microbiome are not well understood. In this study, 12 perennial alfalfa cultivars were grown continuously for eight years. Rhizosphere samples were tested using Illumina sequencing of the 16S rRNA gene coupled with co-occurrence network analysis to explore the relationship between alfalfa (biomass and crude protein content), soil properties, and the microbial composition and diversity. Redundancy analysis showed SOC and pH had the greatest impact on the composition of the rhizosphere microbial community. Moreover, microbial diversity also contributes to microbial composition. Soil properties (AP, EC, SOC and pH) exhibited a significant positive correlation with soil bacterial communities, which was attributed to the differences between plant cultivars. Partial least squares path modeling (PLS-PM) revealed that microbial biomass and community composition rather than diversity, are the dominant determinants in the rhizosphere soil nitrogen content of perennial alfalfa. Our findings demonstrate that the soil microbial biomass and composition of rhizosphere bacterial communities are strongly affected by cultivar, driving the changes in soil nitrogen content, and variances in the selective capacities of plants.https://www.frontiersin.org/articles/10.3389/fpls.2022.938865/fullalfalfa cultivarssoil propertiesrhizosphere soil microbial communitymicrobial diversitysoil nitrogen |
| spellingShingle | Yunru An Haoyang Sun Wei Zhang Yunfu Sun Shuxia Li Zhouchang Yu Rongchen Yang Tianming Hu Peizhi Yang Distinct rhizosphere soil responses to nitrogen in relation to microbial biomass and community composition at initial flowering stages of alfalfa cultivars Frontiers in Plant Science alfalfa cultivars soil properties rhizosphere soil microbial community microbial diversity soil nitrogen |
| title | Distinct rhizosphere soil responses to nitrogen in relation to microbial biomass and community composition at initial flowering stages of alfalfa cultivars |
| title_full | Distinct rhizosphere soil responses to nitrogen in relation to microbial biomass and community composition at initial flowering stages of alfalfa cultivars |
| title_fullStr | Distinct rhizosphere soil responses to nitrogen in relation to microbial biomass and community composition at initial flowering stages of alfalfa cultivars |
| title_full_unstemmed | Distinct rhizosphere soil responses to nitrogen in relation to microbial biomass and community composition at initial flowering stages of alfalfa cultivars |
| title_short | Distinct rhizosphere soil responses to nitrogen in relation to microbial biomass and community composition at initial flowering stages of alfalfa cultivars |
| title_sort | distinct rhizosphere soil responses to nitrogen in relation to microbial biomass and community composition at initial flowering stages of alfalfa cultivars |
| topic | alfalfa cultivars soil properties rhizosphere soil microbial community microbial diversity soil nitrogen |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.938865/full |
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