Functions of CsGPA1 on the hypocotyl elongation and root growth of cucumbers
Abstract G proteins regulate shoot, root, and epidermis development, as well as biotic stress tolerance in plants; however, most studies have examined model plants and less attention has been paid to the function of G proteins in horticultural plants. Here, we identified a G protein, CsGPA1, from cu...
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Nature Portfolio
2018-10-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-018-33782-4 |
| _version_ | 1819200323844046848 |
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| author | Yan Yan Wenna Zhang Yansu Li Chaoxing He Lihong Gao Xianchang Yu |
| author_facet | Yan Yan Wenna Zhang Yansu Li Chaoxing He Lihong Gao Xianchang Yu |
| author_sort | Yan Yan |
| collection | DOAJ |
| description | Abstract G proteins regulate shoot, root, and epidermis development, as well as biotic stress tolerance in plants; however, most studies have examined model plants and less attention has been paid to the function of G proteins in horticultural plants. Here, we identified a G protein, CsGPA1, from cucumber and studied its function in seedling development of cucumbers. CsGPA1 is a peptide of 392 amino acids with a predicted molecular mass of 44.6 kDa. Spatiotemporal expression analysis found that endogenous CsGPA1 was highly expressed in roots and young leaves. Immunohistochemical assay revealed that functional CsGPA1 was present in the plasma membranes of the epidermis and cortex, and in the cytosol of the endodermis, parenchyma, and vasculature of root meristematic cells. In comparison with wild-type seedlings, CsGPA1-overexpressing transgenic lines exhibited enhanced seed germination and earlier seedling development including hypocotyl elongation and root growth. In contrast, RNAi silencing of the CsGPA1 gene inhibited seedling growth and development. Further study showed that CsGPA1 controled hypocotyl elongation and root growth via promoting cell size and the meristem of hypocotyl and root tip cells of cucumber plants. Our study expands the roles of G proteins in plants and helps to elucidate the mechanism by which cucumber regulates early seedling development. |
| first_indexed | 2024-12-23T03:30:25Z |
| format | Article |
| id | doaj.art-f1003c40b37f418e881fa8205d97fdcb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T03:30:25Z |
| publishDate | 2018-10-01 |
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| spelling | doaj.art-f1003c40b37f418e881fa8205d97fdcb2022-12-21T18:01:44ZengNature PortfolioScientific Reports2045-23222018-10-018111210.1038/s41598-018-33782-4Functions of CsGPA1 on the hypocotyl elongation and root growth of cucumbersYan Yan0Wenna Zhang1Yansu Li2Chaoxing He3Lihong Gao4Xianchang Yu5Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural UniversityBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural UniversityThe Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesThe Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural UniversityThe Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesAbstract G proteins regulate shoot, root, and epidermis development, as well as biotic stress tolerance in plants; however, most studies have examined model plants and less attention has been paid to the function of G proteins in horticultural plants. Here, we identified a G protein, CsGPA1, from cucumber and studied its function in seedling development of cucumbers. CsGPA1 is a peptide of 392 amino acids with a predicted molecular mass of 44.6 kDa. Spatiotemporal expression analysis found that endogenous CsGPA1 was highly expressed in roots and young leaves. Immunohistochemical assay revealed that functional CsGPA1 was present in the plasma membranes of the epidermis and cortex, and in the cytosol of the endodermis, parenchyma, and vasculature of root meristematic cells. In comparison with wild-type seedlings, CsGPA1-overexpressing transgenic lines exhibited enhanced seed germination and earlier seedling development including hypocotyl elongation and root growth. In contrast, RNAi silencing of the CsGPA1 gene inhibited seedling growth and development. Further study showed that CsGPA1 controled hypocotyl elongation and root growth via promoting cell size and the meristem of hypocotyl and root tip cells of cucumber plants. Our study expands the roles of G proteins in plants and helps to elucidate the mechanism by which cucumber regulates early seedling development.https://doi.org/10.1038/s41598-018-33782-4Hypocotyl ElongationRNAi SilencingCucumber PlantsEarly SeedlingRoot Meristematic Cells |
| spellingShingle | Yan Yan Wenna Zhang Yansu Li Chaoxing He Lihong Gao Xianchang Yu Functions of CsGPA1 on the hypocotyl elongation and root growth of cucumbers Scientific Reports Hypocotyl Elongation RNAi Silencing Cucumber Plants Early Seedling Root Meristematic Cells |
| title | Functions of CsGPA1 on the hypocotyl elongation and root growth of cucumbers |
| title_full | Functions of CsGPA1 on the hypocotyl elongation and root growth of cucumbers |
| title_fullStr | Functions of CsGPA1 on the hypocotyl elongation and root growth of cucumbers |
| title_full_unstemmed | Functions of CsGPA1 on the hypocotyl elongation and root growth of cucumbers |
| title_short | Functions of CsGPA1 on the hypocotyl elongation and root growth of cucumbers |
| title_sort | functions of csgpa1 on the hypocotyl elongation and root growth of cucumbers |
| topic | Hypocotyl Elongation RNAi Silencing Cucumber Plants Early Seedling Root Meristematic Cells |
| url | https://doi.org/10.1038/s41598-018-33782-4 |
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