KOLEKSI SEL TELUR DENGAN TEKNIK LAPAROSKOPI UNTUK PRODUKSI EMBRIO DAN TRANSFER EMBRIO PADA DOMBA
An experiment was carried out to analyze the application of laparoscopic technique for oocyte collection, in vitro embryo production and embryo transfer in sheep. The first experiment was conducted to observe effect of gonadotropin stimulation on follicle development and laparoscopic technique for o...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bogor Agricultural University
2007-08-01
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| Series: | Jurnal Ilmu Pertanian Indonesia |
| Subjects: | |
| Online Access: | http://journal.ipb.ac.id/index.php/JIPI/article/view/6546 |
| _version_ | 1828229064386674688 |
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| author | Mohamad Agus Setiadi Iman Supriatna Arief Boediono |
| author_facet | Mohamad Agus Setiadi Iman Supriatna Arief Boediono |
| author_sort | Mohamad Agus Setiadi |
| collection | DOAJ |
| description | An experiment was carried out to analyze the application of laparoscopic technique for oocyte collection, in vitro embryo production and embryo transfer in sheep. The first experiment was conducted to observe effect of gonadotropin stimulation on follicle development and laparoscopic technique for oocytes aspiration. In the second experiment, effect of culture system on the embryo development in vitro was assessed and in the third experiment, the application of laparoscopic for embryo transfer has been conducted. The result showed that single dose of gonadotrophin was sufficient to support follicle development significantly and it could help follicle visualization. It also showed that laparoscopic ovum-pick up could be conducted weekly without any restriction The second series experiment showed CR1aa culture system was better than TCM 199 (29.90°/o vs 8.00%) and the changing of media was required to ensure better metabolism process for embryos. The third experiment revealed that embryo transfer could be conducted with an aid from laparoscope. In conclusion, single dose PMSG stimulation is sufficient to support follicle development for /aparoscopic ovum-pick up, the culture media changing affects the successful rate of in vitro embryo production (8% vs 25.66%) and the laparoscopy technique can be used safely for embryo transfer on sheep |
| first_indexed | 2024-04-12T18:27:46Z |
| format | Article |
| id | doaj.art-f102c1ccce9045b7aa5606922901ea86 |
| institution | Directory Open Access Journal |
| issn | 0853-4217 2443-3462 |
| language | English |
| last_indexed | 2024-04-12T18:27:46Z |
| publishDate | 2007-08-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Jurnal Ilmu Pertanian Indonesia |
| spelling | doaj.art-f102c1ccce9045b7aa5606922901ea862022-12-22T03:21:10ZengBogor Agricultural UniversityJurnal Ilmu Pertanian Indonesia0853-42172443-34622007-08-01122116122KOLEKSI SEL TELUR DENGAN TEKNIK LAPAROSKOPI UNTUK PRODUKSI EMBRIO DAN TRANSFER EMBRIO PADA DOMBAMohamad Agus SetiadiIman SupriatnaArief BoedionoAn experiment was carried out to analyze the application of laparoscopic technique for oocyte collection, in vitro embryo production and embryo transfer in sheep. The first experiment was conducted to observe effect of gonadotropin stimulation on follicle development and laparoscopic technique for oocytes aspiration. In the second experiment, effect of culture system on the embryo development in vitro was assessed and in the third experiment, the application of laparoscopic for embryo transfer has been conducted. The result showed that single dose of gonadotrophin was sufficient to support follicle development significantly and it could help follicle visualization. It also showed that laparoscopic ovum-pick up could be conducted weekly without any restriction The second series experiment showed CR1aa culture system was better than TCM 199 (29.90°/o vs 8.00%) and the changing of media was required to ensure better metabolism process for embryos. The third experiment revealed that embryo transfer could be conducted with an aid from laparoscope. In conclusion, single dose PMSG stimulation is sufficient to support follicle development for /aparoscopic ovum-pick up, the culture media changing affects the successful rate of in vitro embryo production (8% vs 25.66%) and the laparoscopy technique can be used safely for embryo transfer on sheephttp://journal.ipb.ac.id/index.php/JIPI/article/view/6546laparoscopicoocyteembryo transfersheep |
| spellingShingle | Mohamad Agus Setiadi Iman Supriatna Arief Boediono KOLEKSI SEL TELUR DENGAN TEKNIK LAPAROSKOPI UNTUK PRODUKSI EMBRIO DAN TRANSFER EMBRIO PADA DOMBA Jurnal Ilmu Pertanian Indonesia laparoscopic oocyte embryo transfer sheep |
| title | KOLEKSI SEL TELUR DENGAN TEKNIK LAPAROSKOPI UNTUK PRODUKSI EMBRIO DAN TRANSFER EMBRIO PADA DOMBA |
| title_full | KOLEKSI SEL TELUR DENGAN TEKNIK LAPAROSKOPI UNTUK PRODUKSI EMBRIO DAN TRANSFER EMBRIO PADA DOMBA |
| title_fullStr | KOLEKSI SEL TELUR DENGAN TEKNIK LAPAROSKOPI UNTUK PRODUKSI EMBRIO DAN TRANSFER EMBRIO PADA DOMBA |
| title_full_unstemmed | KOLEKSI SEL TELUR DENGAN TEKNIK LAPAROSKOPI UNTUK PRODUKSI EMBRIO DAN TRANSFER EMBRIO PADA DOMBA |
| title_short | KOLEKSI SEL TELUR DENGAN TEKNIK LAPAROSKOPI UNTUK PRODUKSI EMBRIO DAN TRANSFER EMBRIO PADA DOMBA |
| title_sort | koleksi sel telur dengan teknik laparoskopi untuk produksi embrio dan transfer embrio pada domba |
| topic | laparoscopic oocyte embryo transfer sheep |
| url | http://journal.ipb.ac.id/index.php/JIPI/article/view/6546 |
| work_keys_str_mv | AT mohamadagussetiadi koleksiseltelurdengantekniklaparoskopiuntukproduksiembriodantransferembriopadadomba AT imansupriatna koleksiseltelurdengantekniklaparoskopiuntukproduksiembriodantransferembriopadadomba AT ariefboediono koleksiseltelurdengantekniklaparoskopiuntukproduksiembriodantransferembriopadadomba |