Study of Enzymatic Hydrolysis of Dilute Acid Pretreated Coconut Husk
Coconut husk is classified as complex lignocellulosic material that contains cellulose, hemicellulose, lignin, and some other extractive compounds. Cellulose from coconut husk can be used as fermentation substrate after enzymatic hydrolysis. In contrary, lignin content from the coconut husk will act...
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| Format: | Article |
| Language: | English |
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Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)
2012-12-01
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| Series: | Bulletin of Chemical Reaction Engineering & Catalysis |
| Subjects: | |
| Online Access: | https://journal.bcrec.id/index.php/bcrec/article/view/19577 |
| _version_ | 1797677788751200256 |
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| author | Rudy Agustriyanto Akbarningrum Fatmawati Yusnita Liasari |
| author_facet | Rudy Agustriyanto Akbarningrum Fatmawati Yusnita Liasari |
| author_sort | Rudy Agustriyanto |
| collection | DOAJ |
| description | Coconut husk is classified as complex lignocellulosic material that contains cellulose, hemicellulose, lignin, and some other extractive compounds. Cellulose from coconut husk can be used as fermentation substrate after enzymatic hydrolysis. In contrary, lignin content from the coconut husk will act as an inhibitor in this hydrolysis process. Therefore, a pretreatment process is needed to enhance the hydrolysis of cellulose. The objective of this research is to investigate the production of the glucose through dilute acid pretreatment and to obtain its optimum operating conditions. In this study, the pretreatment was done using dilute sulfuric acid in an autoclave reactor. The pretreatment condition were varied at 80°C, 100°C, 120°C and 0.9%, 1.2%, 1.5% for temperature and acid concentration respectively. The acid pretreated coconut husk was then hydrolyzed using commercial cellulase (celluclast) and β-glucosidase (Novozyme 188). The hydrolysis time was 72 hours and the operating conditions were varied at several temperature and pH. From the experimental results it can be concluded that the delignification temperature variation has greater influence than the acid concentration. The optimum operating condition was obtained at pH 4 and 50°C which was pretreated at 100°C using 1.5% acid concentration. © 2012 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0) |
| first_indexed | 2024-03-11T22:50:15Z |
| format | Article |
| id | doaj.art-f103e54dcd9447d6a4320b4c7ec270ea |
| institution | Directory Open Access Journal |
| issn | 1978-2993 |
| language | English |
| last_indexed | 2024-03-11T22:50:15Z |
| publishDate | 2012-12-01 |
| publisher | Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS) |
| record_format | Article |
| series | Bulletin of Chemical Reaction Engineering & Catalysis |
| spelling | doaj.art-f103e54dcd9447d6a4320b4c7ec270ea2023-09-22T04:01:03ZengMasyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)Bulletin of Chemical Reaction Engineering & Catalysis1978-29932012-12-017213714110.9767/bcrec.7.2.4046.137-1418221Study of Enzymatic Hydrolysis of Dilute Acid Pretreated Coconut HuskRudy Agustriyanto0Akbarningrum Fatmawati1Yusnita Liasari2Dept. of Chemical Engineering, Surabaya University, Jl. Raya Kalirungkut, Surabaya 60292, IndonesiaDept. of Chemical Engineering, Surabaya University, Jl. Raya Kalirungkut, Surabaya 60292, IndonesiaFaculty of Technobiology, Surabaya University, Jl. Raya Kalirungkut, Surabaya 60292, IndonesiaCoconut husk is classified as complex lignocellulosic material that contains cellulose, hemicellulose, lignin, and some other extractive compounds. Cellulose from coconut husk can be used as fermentation substrate after enzymatic hydrolysis. In contrary, lignin content from the coconut husk will act as an inhibitor in this hydrolysis process. Therefore, a pretreatment process is needed to enhance the hydrolysis of cellulose. The objective of this research is to investigate the production of the glucose through dilute acid pretreatment and to obtain its optimum operating conditions. In this study, the pretreatment was done using dilute sulfuric acid in an autoclave reactor. The pretreatment condition were varied at 80°C, 100°C, 120°C and 0.9%, 1.2%, 1.5% for temperature and acid concentration respectively. The acid pretreated coconut husk was then hydrolyzed using commercial cellulase (celluclast) and β-glucosidase (Novozyme 188). The hydrolysis time was 72 hours and the operating conditions were varied at several temperature and pH. From the experimental results it can be concluded that the delignification temperature variation has greater influence than the acid concentration. The optimum operating condition was obtained at pH 4 and 50°C which was pretreated at 100°C using 1.5% acid concentration. © 2012 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0)https://journal.bcrec.id/index.php/bcrec/article/view/19577coconutenzymehydrolysislignocellulose |
| spellingShingle | Rudy Agustriyanto Akbarningrum Fatmawati Yusnita Liasari Study of Enzymatic Hydrolysis of Dilute Acid Pretreated Coconut Husk Bulletin of Chemical Reaction Engineering & Catalysis coconut enzyme hydrolysis lignocellulose |
| title | Study of Enzymatic Hydrolysis of Dilute Acid Pretreated Coconut Husk |
| title_full | Study of Enzymatic Hydrolysis of Dilute Acid Pretreated Coconut Husk |
| title_fullStr | Study of Enzymatic Hydrolysis of Dilute Acid Pretreated Coconut Husk |
| title_full_unstemmed | Study of Enzymatic Hydrolysis of Dilute Acid Pretreated Coconut Husk |
| title_short | Study of Enzymatic Hydrolysis of Dilute Acid Pretreated Coconut Husk |
| title_sort | study of enzymatic hydrolysis of dilute acid pretreated coconut husk |
| topic | coconut enzyme hydrolysis lignocellulose |
| url | https://journal.bcrec.id/index.php/bcrec/article/view/19577 |
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