Characterization of Divergent Grapevine Badnavirus 1 Isolates Found on Different Fig Species (<i>Ficus</i> spp.)
Fig mosaic disease is spread worldwide and is believed to have a viral etiology. Divergent isolates of grapevine badnavirus 1 (GBV1), named fGBV1, were discovered on <i>Ficus carica</i>, <i>F. palmata</i>, <i>F. virgata</i>, and <i>F. afghanistanica</i>...
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MDPI AG
2022-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/19/2532 |
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| author | Sergei Chirkov Anna Sheveleva Svetlana Tsygankova Fedor Sharko Irina Mitrofanova |
| author_facet | Sergei Chirkov Anna Sheveleva Svetlana Tsygankova Fedor Sharko Irina Mitrofanova |
| author_sort | Sergei Chirkov |
| collection | DOAJ |
| description | Fig mosaic disease is spread worldwide and is believed to have a viral etiology. Divergent isolates of grapevine badnavirus 1 (GBV1), named fGBV1, were discovered on <i>Ficus carica</i>, <i>F. palmata</i>, <i>F. virgata</i>, and <i>F. afghanistanica</i> in the fig germplasm collection of the Nikita Botanical Gardens, Russia, expanding the list of viruses infecting this crop. The complete genomes of five fGBV1 isolates from <i>F. carica</i> and <i>F. palmata</i> trees were determined using high-throughput and Sanger sequencing. The genomes comprised 7283 base pairs, contained four overlapping open reading frames, were 99.7 to 99.9% identical to each other, and related to GBV1 (83.2% identity). The reverse transcriptase RNase H genome regions of fGBV1 and GBV1 share 84.6% identity, indicating that fGBV1 is a divergent isolate of GBV1, which was found on the new natural hosts from a different family (<i>Moraceae</i>). Further, fGBV1-specific primers were developed to detect the virus using RT-PCR. Survey of 47 trees, belonging to four fig species and 14 local and introduced <i>F. carica</i> cultivars, showed the high fGBV1 prevalence in the collection (93.6%), including trees with no obvious symptoms of fig mosaic disease. |
| first_indexed | 2024-03-09T21:18:23Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T21:18:23Z |
| publishDate | 2022-09-01 |
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| series | Plants |
| spelling | doaj.art-f1053b67b4454ea3bc2b1309a4db4ade2023-11-23T21:29:01ZengMDPI AGPlants2223-77472022-09-011119253210.3390/plants11192532Characterization of Divergent Grapevine Badnavirus 1 Isolates Found on Different Fig Species (<i>Ficus</i> spp.)Sergei Chirkov0Anna Sheveleva1Svetlana Tsygankova2Fedor Sharko3Irina Mitrofanova4Department of Virology, Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, RussiaDepartment of Virology, Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, RussiaNational Research Center “Kurchatov Institute”, 123182 Moscow, RussiaNational Research Center “Kurchatov Institute”, 123182 Moscow, RussiaTsitsin Main Botanical Garden of Russian Academy of Sciences, 127276 Moscow, RussiaFig mosaic disease is spread worldwide and is believed to have a viral etiology. Divergent isolates of grapevine badnavirus 1 (GBV1), named fGBV1, were discovered on <i>Ficus carica</i>, <i>F. palmata</i>, <i>F. virgata</i>, and <i>F. afghanistanica</i> in the fig germplasm collection of the Nikita Botanical Gardens, Russia, expanding the list of viruses infecting this crop. The complete genomes of five fGBV1 isolates from <i>F. carica</i> and <i>F. palmata</i> trees were determined using high-throughput and Sanger sequencing. The genomes comprised 7283 base pairs, contained four overlapping open reading frames, were 99.7 to 99.9% identical to each other, and related to GBV1 (83.2% identity). The reverse transcriptase RNase H genome regions of fGBV1 and GBV1 share 84.6% identity, indicating that fGBV1 is a divergent isolate of GBV1, which was found on the new natural hosts from a different family (<i>Moraceae</i>). Further, fGBV1-specific primers were developed to detect the virus using RT-PCR. Survey of 47 trees, belonging to four fig species and 14 local and introduced <i>F. carica</i> cultivars, showed the high fGBV1 prevalence in the collection (93.6%), including trees with no obvious symptoms of fig mosaic disease.https://www.mdpi.com/2223-7747/11/19/2532<i>Ficus</i> spp.badnavirusgrapevine badnavirus 1high-throughput sequencing |
| spellingShingle | Sergei Chirkov Anna Sheveleva Svetlana Tsygankova Fedor Sharko Irina Mitrofanova Characterization of Divergent Grapevine Badnavirus 1 Isolates Found on Different Fig Species (<i>Ficus</i> spp.) Plants <i>Ficus</i> spp. badnavirus grapevine badnavirus 1 high-throughput sequencing |
| title | Characterization of Divergent Grapevine Badnavirus 1 Isolates Found on Different Fig Species (<i>Ficus</i> spp.) |
| title_full | Characterization of Divergent Grapevine Badnavirus 1 Isolates Found on Different Fig Species (<i>Ficus</i> spp.) |
| title_fullStr | Characterization of Divergent Grapevine Badnavirus 1 Isolates Found on Different Fig Species (<i>Ficus</i> spp.) |
| title_full_unstemmed | Characterization of Divergent Grapevine Badnavirus 1 Isolates Found on Different Fig Species (<i>Ficus</i> spp.) |
| title_short | Characterization of Divergent Grapevine Badnavirus 1 Isolates Found on Different Fig Species (<i>Ficus</i> spp.) |
| title_sort | characterization of divergent grapevine badnavirus 1 isolates found on different fig species i ficus i spp |
| topic | <i>Ficus</i> spp. badnavirus grapevine badnavirus 1 high-throughput sequencing |
| url | https://www.mdpi.com/2223-7747/11/19/2532 |
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