Functional nucleic acids with synthetic sugar or nucleobase moieties
Functional nucleic acids including aptamers and DNAzymes are a class of valuable molecular tool for biotechnology. However, DNA and RNA aptamers and catalysts suffer from low biological stability and limited chemical diversity. Xeno-nucleic acids (XNAs) refer to nucleic acid analogues containing sug...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-09-01
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| Series: | Advanced Agrochem |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773237123000564 |
| _version_ | 1827302652691611648 |
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| author | Ze Zhang Siqi Chen Zhe Li Hanyang Yu |
| author_facet | Ze Zhang Siqi Chen Zhe Li Hanyang Yu |
| author_sort | Ze Zhang |
| collection | DOAJ |
| description | Functional nucleic acids including aptamers and DNAzymes are a class of valuable molecular tool for biotechnology. However, DNA and RNA aptamers and catalysts suffer from low biological stability and limited chemical diversity. Xeno-nucleic acids (XNAs) refer to nucleic acid analogues containing sugar moieties that are structurally distinct from DNA and RNA and possess advantageous properties. In this article, we first focus on two types of XNAs, threose nucleic acid (TNA) and 2’-fluoroarabinose nucleic acid (FANA), and summarize recent in vitro selections of TNA and FANA aptamers and catalysts. We then review three classes of unnatural base pairs (UBPs) and highlight examples of UBP-containing DNA aptamers and DNAzymes. Lastly, we briefly describe an XNA-modified DNAzyme 10–23 (X10-23) and its application in RNA knockdown and virus detection. Functional XNAs provide important chemical biology tools for biomedical research and future interdisciplinary collaboration will boost XNA basic research and clinical translation. |
| first_indexed | 2024-04-24T16:48:59Z |
| format | Article |
| id | doaj.art-f1056b8f65634f64b730bc4a7e0d78bd |
| institution | Directory Open Access Journal |
| issn | 2773-2371 |
| language | English |
| last_indexed | 2024-04-24T16:48:59Z |
| publishDate | 2023-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Advanced Agrochem |
| spelling | doaj.art-f1056b8f65634f64b730bc4a7e0d78bd2024-03-29T05:51:42ZengKeAi Communications Co., Ltd.Advanced Agrochem2773-23712023-09-0123236245Functional nucleic acids with synthetic sugar or nucleobase moietiesZe Zhang0Siqi Chen1Zhe Li2Hanyang Yu3State Key Laboratory of Coordination Chemistry, Department of Biomedical Engineering, College of Engineering and Applied Sciences, Chemistry and Biomedicine Innovation Center (ChemBIC), Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, Jiangsu, 210023, ChinaState Key Laboratory of Coordination Chemistry, Department of Biomedical Engineering, College of Engineering and Applied Sciences, Chemistry and Biomedicine Innovation Center (ChemBIC), Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, Jiangsu, 210023, ChinaState Key Laboratory of Analytical Chemistry for Life Science, Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing, 210023, ChinaState Key Laboratory of Coordination Chemistry, Department of Biomedical Engineering, College of Engineering and Applied Sciences, Chemistry and Biomedicine Innovation Center (ChemBIC), Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, Jiangsu, 210023, China; Corresponding author.Functional nucleic acids including aptamers and DNAzymes are a class of valuable molecular tool for biotechnology. However, DNA and RNA aptamers and catalysts suffer from low biological stability and limited chemical diversity. Xeno-nucleic acids (XNAs) refer to nucleic acid analogues containing sugar moieties that are structurally distinct from DNA and RNA and possess advantageous properties. In this article, we first focus on two types of XNAs, threose nucleic acid (TNA) and 2’-fluoroarabinose nucleic acid (FANA), and summarize recent in vitro selections of TNA and FANA aptamers and catalysts. We then review three classes of unnatural base pairs (UBPs) and highlight examples of UBP-containing DNA aptamers and DNAzymes. Lastly, we briefly describe an XNA-modified DNAzyme 10–23 (X10-23) and its application in RNA knockdown and virus detection. Functional XNAs provide important chemical biology tools for biomedical research and future interdisciplinary collaboration will boost XNA basic research and clinical translation.http://www.sciencedirect.com/science/article/pii/S2773237123000564 |
| spellingShingle | Ze Zhang Siqi Chen Zhe Li Hanyang Yu Functional nucleic acids with synthetic sugar or nucleobase moieties Advanced Agrochem |
| title | Functional nucleic acids with synthetic sugar or nucleobase moieties |
| title_full | Functional nucleic acids with synthetic sugar or nucleobase moieties |
| title_fullStr | Functional nucleic acids with synthetic sugar or nucleobase moieties |
| title_full_unstemmed | Functional nucleic acids with synthetic sugar or nucleobase moieties |
| title_short | Functional nucleic acids with synthetic sugar or nucleobase moieties |
| title_sort | functional nucleic acids with synthetic sugar or nucleobase moieties |
| url | http://www.sciencedirect.com/science/article/pii/S2773237123000564 |
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